ZEISS Elyra 7​

Due to the fine structures of cytoskeleton components, for example the actin network or microtubular filaments, imaging far below 100 nm is often performed with super-resolution techniques. Lattice SIM² allows you to gain much more structural information from your samples compared to conventional SIM techniques. It not only operates with a resolution of down to 60 nm but also provides markedly improved sectioning quality.

Due to its unique lattice structural illumination, Elyra 7 combines high speed imaging with incredible light efficiency, low photon dosage and sensitivity. You can observe cellular, subcellular, and even sub-organelle structures in living specimens in 2D and 3D over time. Whether you are interested in the dynamics of mitochondrial movement, fusion and fission or budding of the endoplasmic reticulum, Elyra 7 Lattice SIM² provides you with the necessary live cell compatibility at super-resolution.

SIM² Apotome is your flexible live cell imaging method for experiments that do not require the highest spatial resolution but rely instead on excellent sectioning quality. SIM² Apotome is superior to conventional confocal microscopy in terms of lateral and axial resolution as well as volume acquisition speed while it is also very gentle to your sample. The high NA (1.4) 40× magnification images almost reach the resolution and sectioning capabilities of a conventional SIM microscope, while multiplying acquisition speed.

The high-speed performance of SIM Apotome acquisition mode allows for fast tile scan imaging of very large areas at excellent sectioning quality. A mulberry section of 11.1 mm² × 11 µm size was imaged using Nyquist sampling in all three directions and in two colors in less than 2 minutes. Similar speeds also were achieved for a cross section of a leaf.

The need for higher imaging speeds and decreased light dosage is almost unlimited. The robustness of Elyra 7 structured illumination patterns plus the image reconstruction software allow a significant reduction to the number of phase images required for both Lattice SIM and SIM Apotome acquisition modes, while decreasing the resolution only slightly. Lattice SIM acquisition can be operated at 9 phase images per frame while 3 phase images per frame are sufficient for SIM Apotome, increasing the imaging speed by 44 % and 66 %, respectively.

Despite being a structured illumination-based microscope, Elyra 7 Lattice SIM² as well as SIM² Apotome also provide you with super-resolution and high-quality sectioning in thick or scattering samples. The combination of robust illumination patterns and excellent image reconstruction technology enabled us to image throughout an entire murine brain section of ~80 µm thickness expressing the neuronal marker Thy1-eGFP.

You can investigate living or fixed, small or large, thin or thick specimens using Elyra 7 Lattice SIM², SIM² Apotome or SMLM modes. Whether you study vesicle dynamics in cells or yeast or you want to unravel the architecture of plants, C. elegans, zebrafish, D. melanogaster or bacteria, with Elyra 7 you will experience easily accessible super-resolution imaging for your favorite model organism and many other specimens.

Biological samples often contain completely different types of information at different length scales. Being able to collect low to high resolution data in the same sample not only makes you more productive but also interconnects the findings and puts them in context to give you the whole picture.

SMLM encompasses techniques such as PALM, dSTORM, and PAINT. With high power lasers across the visible spectrum and dual camera detection, Elyra 7 allows researchers to gain access to a broad range of dyes, markers and fluorophores in almost any possible combination.

• Resolve Molecular Structures -  SMLM allows you to map precise locations of individual proteins.
  
• Determine the Relationships Between Molecules -  Detect two channels with molecular precision.
• Capture Information in Three Dimensions -  Untangle molecular relationships in z with confidence.