Microscopy Solutions for Sample Preparation

Model organisms can be bacteria, fungi, plants or animals. Ideally, model organisms should exhibit certain characteristics:

• Share many genes with humans
  
• Small size and easy to grow in the lab
  
• Visible congenital traits
  
• Sequenced genome available
• Easy genetic manipulation
  
• Genetically tractable
  
• Short generation times and life cycle

There are two types of stereo microscopes – Greenough and Common Main Objective (CMO) – each with its own special characteristics. The CMO-type provides certain advantages over the Greenough-type, particularly when it comes to illumination, fluorescence capabilities, digital image documentation and ergonomics. Greenough stereo microscopes, however, are more compact, highly integrated and economical in price.

Scientists and technicians can be supported in their daily routines by a 3D stereoscopic view combined with a large free-working distance and ergonomic features. These microscopes are perfect for mechanical manipulation, sorting, and general sample preparation. Stereo or zoom microscopes are also used preferentially for fluorescence screening of modified genotypes (for example, through CRISPR/Cas9 mediated mutations) in transgenic studies using markers such as the green (GFP) or red (RFP) fluorescent protein. Combining a large field of view with high resolution will speed up this process significantly.

Dissecting microscopes are also widespread in neuroscience – say, when dealing with larger vertebrate animals such as rats, which often serve as a source of primary cell cultures.