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Workshop

ZEISS@Center for Regenerative Therapies

Automated Microscopy
Charité - Universitätsmedizin Berlin, Campus Virchow-Klinikum, Föhrer Str. 15, 13353 Berlin
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Two Technologies in One System

ZEISS Celldiscoverer 7 for Reproducible Widefield and Confocal Imaging

Fluorescence imaging is used in a wide application range from immunohistochemical labeling of fixed samples up to tagging dynamic molecules in living cells using fluorescence proteins. Samples can vary strongly from thick organoids to thinnest cell extensions like lamellipodia and filopodia. Furthermore, specimen can be mounted on a large variety of sample carriers from standard slides to dedicated 96 wells.

To cover this huge variety of samples and applications a flexible imaging tool is needed that guarantees reliable results. ZEISS Celldiscoverer 7 with LSM 900 Airyscan 2 offers the necessary options for fast and gentle fluorescence imaging in widefield or confocal mode – mixed mode imaging is also possible. Sample carriers are automatically detected. The system calibrates automatically the optical system to the used sample bottom to guarantee highest image quality and reliability. The system is completely controlled by the ZEN software for daily easy-to-use routine microscopy and screening applications.

In this workshop we will present the technical features and possible applications of Celldiscoverer 7 followed by detailed hands-on sessions.

Key Learnings

  • How to combine the different imaging modes, including transmitted light, wide-field fluorescence, and confocal imaging.
  • The optimal confocal setup for automated live cell imaging and how to use it in screening applications.
  • The performance increase in resolution with LSM Airyscan.
  • Experience up to 90nm resolution with Airyscan Joint Deconvolution (jDCV).
  • The benefits of Connected Microscopy workflows to keep image data in context.

Agenda

  • 2 - 3 PM

    Automated Microscopy - Two Technologies in One System for Automatization
    ZEISS Celldiscoverer 7 for reproducible Widefield and Confocal Imaging
  • Book your demo

    10 AM - 5 PM

    We are happy to show you   ZEISS Celldiscoverer 7  and answer your questions in a 2 hour one-on-one demonstration with your own samples. We will contact you to coordinate a slot.
Location

1. Center for Regenerative Therapies - Lecture

Charité - Universitätsmedizin Berlin Föhrer Str. 15, Room 0.044/45 EG 13353 Berlin Germany
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+49 (0)30 450 539 500
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2. Center for Regenerative Therapies - Demo

Charité - Universitätsmedizin Berlin Föhrer Str. 15, Room 0.0095 EG 13353 Berlin Deutschland
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ZEISS Celldiscoverer 7 at Work

  • 384 microwell plate imaged with different magnifications in 3 channels.
  • HeLa Kyoto cell growth assay imaged every 15 minutes for 72 hours using Autoimmersion.
  • Lateral line primordium migration and deposition of immature neuromasts in a Zebrafish embryo. Overview image: Phase Gradient Contrast with fluorescence acquisition. High-resolution images: Airyscan 2 Multiplex.
  • Rat cortical primary neuron culture. Deconvolved images using GPU-based deconvolution.
  • Expansion microscopy in mouse brain. Left: whole brain; top right: axon bundles, bottom right: pyramidal cells.
  • Growth of cultured cells imaged over 72 hours using phase gradient contrast. To quantify proliferation, cell region was detected using machine learning. The growth curve shows relative cell coverage over time
  • Living cricket embryos mounted in low-melt agarose, expressing nuclear-localized GFP, multi-position experiment.

    Growth of cultured cells imaged over 72 hours using phase gradient contrast. To quantify proliferation, cell region was detected using machine learning. The growth curve shows relative cell coverage over time. Sample and assay courtesy of P. Denner, Core Research Facilities, German Center of Neurodegenerative Diseases (DZNE), Bonn, Germany.
  • Starlet sea anemone (Nematostella vectensis) imaged with Airyscan 2 high sensitivity mode.

    Starlet sea anemone (Nematostella vectensis) imaged with Airyscan 2 high sensitivity mode. Sample courtesy of A. Stokkermans, Ikmi Group, EMBL, Heidelberg, Germany.
  • 384 microwell plate imaged with different magnifications in 3 channels.

    384 microwell plate imaged with different magnifications in 3 channels. Sample courtesy of P. Denner, Core Research Facilities, German Center of Neurodegenerative Diseases (DZNE), Bonn, Germany.
  • HeLa Kyoto cell growth assay imaged every 15 minutes for 72 hours using Autoimmersion.

    HeLa Kyoto cell growth assay imaged every 15 minutes for 72 hours using Autoimmersion. Sample courtesy of I. Charapitsa, Chemical Biology Core Facility, EMBL, Heidelberg, Germany.
  • Lateral line primordium migration and deposition of immature neuromasts in a Zebrafish embryo. Overview image: Phase Gradient Contrast with fluorescence acquisition. High-resolution images: Airyscan 2 Multiplex.

    Lateral line primordium migration and deposition of immature neuromasts in a Zebrafish embryo. Overview image: Phase Gradient Contrast with fluorescence acquisition. High-resolution images: Airyscan 2 Multiplex. Sample courtesy of J. Hartmann and D. Gilmour, EMBL, Heidelberg, Germany.
  • Rat cortical primary neuron culture. Deconvolved images using GPU-based deconvolution.

    Rat cortical primary neuron culture. Deconvolved images using GPU-based deconvolution. Sample courtesy of H. Braun, LSM Bioanalytik GmbH, Magdeburg, Germany.
  • Expansion microscopy in mouse brain. Left: whole brain; top right: axon bundles, bottom right: pyramidal cells.

    Expansion microscopy in mouse brain. Left: whole brain; top right: axon bundles, bottom right: pyramidal cells. Sample courtesy of S. Asano, Boyden lab, MIT, Cambridge, USA.
  • Growth of cultured cells imaged over 72 hours using phase gradient contrast. To quantify proliferation, cell region was detected using machine learning. The growth curve shows relative cell coverage over time

    Growth of cultured cells imaged over 72 hours using phase gradient contrast. To quantify proliferation, cell region was detected using machine learning. The growth curve shows relative cell coverage over time. Sample and assay courtesy of P. Denner, Core Research Facilities, German Center of Neurodegenerative Diseases (DZNE), Bonn, Germany.
  • Living cricket embryos mounted in low-melt agarose, expressing nuclear-localized GFP, multi-position experiment.

    Living cricket embryos mounted in low-melt agarose, expressing nuclear-localized GFP, multi-position experiment. Sample courtesy of S. Donoughe, Biological Labs, Harvard University, Cambridge, USA.
  • Starlet sea anemone (Nematostella vectensis) imaged with Airyscan 2 high sensitivity mode.

    Starlet sea anemone (Nematostella vectensis) imaged with Airyscan 2 high sensitivity mode. Sample courtesy of A. Stokkermans, Ikmi Group, EMBL, Heidelberg, Germany.