Science/AAAS Ebook

From 3D Light to 3D Electron Microscopy

Shared experiences from the volume EM community

In this new 28-page Science/AAAS ebook, learn about volume electron microscopy (vEM) and volume correlative light and electron microscopy (vCLEM) for life science research.

Get an introduction to the community, read articles, watch videos about vEM and vCLEM workflows, and get inspired from a discussion among electron microscopy experts from both academia and industry.

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Introduction to vEM/vCLEM and its community
Selected articles & videos, describing vEM and vCLEM workflows
Panel discussion among electron microscopy experts from both academia and industry


Sharing the science behind 3D electron microscopy	Sean Sanders (Science/AAAS, Washington, DC, USA)
Community under the microscope	Alexandra Elli, Daniel Hartmann, and Bernhard Zimmermann (Carl Zeiss Microscopy GmbH, Jena, Germany)
Sample preparation for volume correlative light and electron microscopy	Jean Daraspe, Olivia Muriel, Sophie G. Martin, Christel Genoud (UNIL, EMF, Lausanne, Switzerland)
Breaking down the correlative workflow for array tomography: From section collection to image analysis	Ian J. White¹, Steve Furzeland² Jemima J. Burden¹(¹UCL, LMCB, London, UK, ²ZEISS RMS, Cambourne, UK))
X-ray targeting of Arabidopsis pollen tetrads for SBF-SEM	Anna Kremer¹, Kimberly Meechan², Marianne Beckwith², Saskia Lippens¹ (¹VIB, Ghent, Belgium, ²EMBL, Heidelberg, Germany)
Cryo-correlative light and electron microscopy for cellular tomography	Anna M. Steyer, Zhengyi Yang, Simone Mattei (EMBL, Heidelberg, Germany)
Fluorescence-based targeting of a plankton cell for FIB-SEM	Karel Mocaer, Giulia Mizzon, Manuel Gunkel, Aliaksandr Halavatyi, Yannick Schwab, Paolo Ronchi (EMBL, Heidelberg, Germany)
Introducing high-throughput serial section acquisition for ZEISS MultiSEM	Anna Lena Eberle, Tomasz Garbowski, Stephan Nickell (Carl Zeiss MultiSEM GmbH, Oberkochen, Germany)
Panel discussion: vEM/vCLEM community building	Johanna Bischof (Euro-BioImaging Bio-Hub, EMBL, Germany), Lucy Collinson (The Francis Crick Institute, UK), Kedar Narayan (NIH-NCI, USA), Yannick Schwab (EMBL, Germany), Paul Verkade (University of Bristol, UK), Alexandra Elli (Carl Zeiss Microscopy, Germany), Rosy Manser (Carl Zeiss Microscopy, UK)