ZEISS LSM Airyscan

The fundamental purpose of any microscope is to reveal the unknown by resolving the smallest structures. Super-resolution has become standard in florescent imaging and is routinely employed in many microscopy experiments. However, it is crucial to select a method that is safe for living samples and yields reliable results. You don't need to be a microscopy expert to use Airyscan for your super-resolution experiments. Sample preparation and workflows remain unchanged from established confocal imaging practices. With Airyscan, you capture more structural information and collect available fluorescence signal more efficiently, which makes this super-resolution method particularly gentle to your delicate samples. Choose from a variety of processing options and easily customize them to get reliable and quantifiable data. Lateral resolution down to 90 nm is made possible by Joint Deconvolution – utilizing the additional spatial information that only Airyscan can provide.

To understand dynamic processes in living systems, spatial information must not be compromised in favor of the necessary temporal resolution. The ability to combine fast imaging with super-resolution makes Airyscan a versatile tool to observe live dynamics at subcellular resolution and efficiently image large 3D samples, enabling efficient imaging of processes in cells, spheroids, organoids, or whole organisms. The multi-element Airyscan detector facilitates rapid acquisition of 2 to 8 image lines, speeding up the imaging process and improving the acquisition of structural information. Airyscan’s various parallelization options provide optimal flexibility to meet diverse experimental needs. By leveraging the unique information from the area detector, resolution can be further enhanced by Joint Deconvolution, a reliable processing method specifically optimized for Airyscan's high-speed imaging modes.

ZEISS Dynamics Profiler uncovers molecular concentration, diffusion, and flow dynamics of fluorescent proteins in your living samples in a single, easy measurement. By uniquely using the Airyscan detector elements, Dynamics Profiler collects more information than was previously possible with conventional Fluorescence Correlation Spectroscopy (FCS), enabling accurate and reproducible analyses, even with bright samples. On the other hand, delicate samples can also be explored, without excessive light exposure or prolonged experiment time. Develop an in-depth profile of the molecules in your current experiments, from cell cultures to organoids to whole organisms. For example, study the transition of cellular condensates formed by liquid-liquid phase separation as measured by Asymmetric Diffusion. Or, with Flow Analysis, measure speed and direction of fluorescent molecules moving in a bloodstream or in microfluidic systems, such as organs-on-a-chip. Adding molecular dynamics measurements to any of your live sample experiments has never been easier.