ZEISS Lattice Lightsheet 7

COS-7 cells transiently transfected with Calnexin-mEmerald and EB3-tdTomato. EB3 labels the growing ends of microtubules and is necessary for the regulation of microtubule dynamics. Calnexin is a protein of the ER where proteins are synthesized. One volume every 7 sec; continuous imaging for 24 mins. Imaged volume: 118 × 113 × 22 μm3. 240,600 images, 401 volume planes for 300 time points.

Time lapse movie showing dynamics of a U2OS cell stably expressing Actin-GFP (cytoskeleton, cyan). Cells were also labeled with MitoTracker™ Red CMXRos (Mitochondria, green) and Draq 5 (Nucleus, magenta).

U2OS cell expressing Lifeact-tdTomato undergoing mitosis during continuous imaging. Maximum intensity projection. The cell was imaged constantly for 2.5 hours; one volume (113 × 90 × 11 μm³) every 2.2 secs. A total of 1,404,000 images was recorded; 351 volume planes for 4,000 time points.

COS-7 cells transfected with ER-targeted StayGold fluorescence protein. Maximum intensity projection. Recorded with 1 ms exposure time for 40 min continuously. 802,000 images in total. One volume (105 × 56 × 14 µm³) per 1.1 seconds.  
Sample courtesy: Miyawaki Atsushi, RIKEN Institute, Japan

U2OS cells expressing Lifeact-tdTomato and stained with MitoTracker Green. Top row: single-camera configuration. Bottom row: dual-camera configuration. Crosstalk is minimized. In addition, twice as many images can be acquired, resulting in a doubling of temporal resolution.

Color-coded depth projection and maximum intensity projection side- by-side. The T cell was imaged constantly for over 1 hr; one volume every 2.5 secs. Sample: courtesy of M. Fritzsche, University of Oxford, UK.

Fixed mouse germinal vesicle oocytes stained for the nuclear envelope (anti-lamin, cyan), actin (phalloidin, magenta), and microtubules (anti-tubulin, yellow). The Sinc3 15 × 650 lattice light sheet was used for high-resolution imaging of microtubule and actin structures. Follow the 3D structure of the microtubules in the movie. Sample: courtesy of C. So, MPI Göttingen, Germany.

Fixed mouse germinal vesicle oocytes stained for the nuclear envelope (anti-lamin, cyan), actin (phalloidin, magenta), and microtubules (anti-tubulin, yellow). The Sinc3 100 × 1,800 lattice light sheet was used for imaging of the whole oocyte. Sample: courtesy of C. So, MPI Göttingen, Germany.

DeltaD-YFP transgenic zebrafish embryo (Liao et al. 2016, Nature Communications). Fusion protein driven by a transgene containing the endogenous regulatory regions, expression in the tailbud and pre-somitic mesoderm. Signal visible in the cell cortex, and in puncta corresponding to trafficking vesicles (green). Nuclei in magenta. The embryo was imaged for 5 minutes constantly; one volume (150 × 50 × 90 μm3) every 8 sec. Sample: courtesy of Prof. Andrew Oates, EPFL, Switzerland.

Volumetric imaging of trafficking mRNA molecules (green). Nuclei are shown in magenta. Data is displayed as maximum intensity projection. One volume (86 × 80 × 12 μm3) was recorded every 2.5 sec. Sample: courtesy of Prof. Andrew Oates, EPFL, Switzerland.

C. elegans embryo stained for nuclei. The movie shows a color-coded depth projection of the embryo. The embryo was imaged for 10+ minutes constantly; one volume every 700 msec. Imaged volume: 115 × 50 × 30 μm³. A total of 101.000 images was recorded; 101 volume planes for 1000 time points. Customer sample.

C. elegans embryo stained for nuclei. The movie shows a color-coded depth projection of the embryo. The embryo was imaged for 19+ hrs every 5 mins and can be observed going through its normal sleep-wake cycle. Imaged volume: 115 × 50 × 30 μm³. A total of 23,836 images was recorded; 101 volume planes for 236 time points. Customer sample.

C. elegans embryo at the late bean stage (~400 min post fertilization) with ~560 nuclei marked with HIS-58::mCherry (magenta) and centrioles marked by GFP::SAS-7 (green). Cells in mitosis show condensed signal of HIS-58::mCherry and centrioles at spindle poles. Sample: courtesy of N. Kalbfuss, Göncy Lab, EPFL, Switzerland.

Pollen tube stained for mitochondria (MitoTracker Green, green) and Lysosomes (Lysotracker Red, red). Watch the pollen tube extend from the crack in the pollen grain (visualized by its autofluorescence). Mitochondria don't quite advance to the very tip of the pollen tube but stop a few microns before the tip. Rendering of the data set was performed in arivis Vision4D^®. Sample: courtesy of R. Whan, UNSW, Sydney, Australia.

Watch mitochondrial dynamics inside the pollen tube. Mitochondria move towards the tip at the edges and back in the middle of the tube. While trafficking, mitochondria constantly fuse and divide for repair processes and to share and distribute biological molecules. Sample: courtesy of R. Whan, UNSW, Sydney, Australia.