Bio Apps Image Analysis Tools for Cell Biology and Cancer Research in Life Sciences

This app counts cells based on detecting single nuclei via nuclear markers or via phase contrast. It is suitable for end-point assays like proliferation assays, survival assays, trans-well migration assays or any other assay that determines cell numbers in a particular location.

This app determines the area of the field of view that is overgrown with cells, mainly via image pixel variance from phase contrast or fluorescent channels. The app was developed for scratch assays in particular but may also be used to determine cell growth in general.

This app is used to determine any protein expressions in or near a single cell's nucleus. The measurement area is determined by a flexibly adjustable distance and width from the nucleus' outer membrane, allowing precise analyses in arbitrary cellular compartments. Various gene expression or reporter signals are all covered, typically in transfection, reporter, apoptosis, or similar assays.

Spot detection allows quantification of any cellular signals that comes in the shape of speckles. Via a flexible measurement area adjustable in distance and width around the nuclear membrane, arbitrary sub-areas of the cell can be measured. Spot detection is suitable for counting organelles, nuclear speckles, nuclear sub compartments, and similar objects.