How-to Video
How-to Acquire a Multichannel Fluorescence Image
With Labscope and Smart Microscopy
22 February 2021
· 3 min watch
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/authors/michael-goegler.jpg/_jcr_content/renditions/original.image_file.100.100.file/michael-goegler.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/authors/michael-goegler.jpg/_jcr_content/renditions/original./michael-goegler.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/authors/michael-goegler.jpg/_jcr_content/renditions/original./michael-goegler.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/authors/michael-goegler.jpg/_jcr_content/renditions/original./michael-goegler.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/authors/michael-goegler.jpg/_jcr_content/renditions/original./michael-goegler.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/authors/michael-goegler.jpg/_jcr_content/renditions/original./michael-goegler.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/authors/michael-goegler.jpg/_jcr_content/renditions/original./michael-goegler.jpg"})
Author
Dr. Michael Gögler
Market Sector Manager for Routine Microscopy
ZEISS Microscopy
ZEISS Microscopy
Abstract
Smart and Streamlined Digital Microscopy for the Clinical Laboratory
If you work with fluorescently labeled cells or other samples that contain multiple fluorophores, easy image acquisition in independent channels and the overlay thereof is highly desired. Especially with manual microscopes this procedure can be cumbersome.
Key Learnings:
- Learn how to acquire up to 4 fluorescence channels and 1 transmitted light channel in an easy and effortless way.
- Learn how to use a smart fluorescence microscope such as ZEISS Axioscope 5 with Colibri 3 or Axiolab 5, in combination with the microscope camera Axiocam 202 mono and the imaging app Labscope.
- Learn how to adapt the histogram, compare separate channels, and even include them in a report.
![Deutsches Museum Munich - On the Trail of Plasticizers]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/on-the-trail-of-plasticizers/restoration-research_axio-scope-a1_1920px.jpg/_jcr_content/renditions/original.image_file.100.56.0,298,1920,1378.file/restoration-research_axio-scope-a1_1920px.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/on-the-trail-of-plasticizers/restoration-research_axio-scope-a1_1920px.jpg/_jcr_content/renditions/original.image_file.360.203.0,298,1920,1378.file/restoration-research_axio-scope-a1_1920px.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/on-the-trail-of-plasticizers/restoration-research_axio-scope-a1_1920px.jpg/_jcr_content/renditions/original.image_file.768.432.0,298,1920,1378.file/restoration-research_axio-scope-a1_1920px.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/on-the-trail-of-plasticizers/restoration-research_axio-scope-a1_1920px.jpg/_jcr_content/renditions/original.image_file.1024.576.0,298,1920,1378.file/restoration-research_axio-scope-a1_1920px.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/on-the-trail-of-plasticizers/restoration-research_axio-scope-a1_1920px.jpg/_jcr_content/renditions/original.image_file.1280.720.0,298,1920,1378.file/restoration-research_axio-scope-a1_1920px.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/on-the-trail-of-plasticizers/restoration-research_axio-scope-a1_1920px.jpg/_jcr_content/renditions/original.image_file.1440.810.0,298,1920,1378.file/restoration-research_axio-scope-a1_1920px.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/on-the-trail-of-plasticizers/restoration-research_axio-scope-a1_1920px.jpg/_jcr_content/renditions/original.image_file.1920.1080.0,298,1920,1378.file/restoration-research_axio-scope-a1_1920px.jpg"} "Deutsches Museum Munich - On the Trail of Plasticizers")
![Webinar Thumbnail]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/thumbnails/mastering-urine-microscopy-techniques.jpg/_jcr_content/renditions/original.image_file.100.56.0,0,1905,1074.file/mastering-urine-microscopy-techniques.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/thumbnails/mastering-urine-microscopy-techniques.jpg/_jcr_content/renditions/original.image_file.360.203.0,0,1905,1074.file/mastering-urine-microscopy-techniques.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/thumbnails/mastering-urine-microscopy-techniques.jpg/_jcr_content/renditions/original.image_file.768.432.0,0,1905,1074.file/mastering-urine-microscopy-techniques.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/thumbnails/mastering-urine-microscopy-techniques.jpg/_jcr_content/renditions/original.image_file.1024.576.0,0,1905,1074.file/mastering-urine-microscopy-techniques.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/thumbnails/mastering-urine-microscopy-techniques.jpg/_jcr_content/renditions/original.image_file.1280.720.0,0,1905,1074.file/mastering-urine-microscopy-techniques.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/thumbnails/mastering-urine-microscopy-techniques.jpg/_jcr_content/renditions/original.image_file.1440.810.0,0,1905,1074.file/mastering-urine-microscopy-techniques.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/thumbnails/mastering-urine-microscopy-techniques.jpg/_jcr_content/renditions/original.image_file.1905.1072.0,0,1905,1074.file/mastering-urine-microscopy-techniques.jpg"} "Webinar Thumbnail: Microscopy in Urinalysis: Observe Urine, Detect Abnormalities")
![Maria Cuacos | Crop Research at IPK Gatersleben]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/breeding-research-on-the-way-to-a-plant-based-bioeconomy/maria-cuacos_1920px.jpg/_jcr_content/renditions/original.image_file.100.56.0,234,1920,1314.file/maria-cuacos_1920px.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/breeding-research-on-the-way-to-a-plant-based-bioeconomy/maria-cuacos_1920px.jpg/_jcr_content/renditions/original.image_file.360.203.0,234,1920,1314.file/maria-cuacos_1920px.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/breeding-research-on-the-way-to-a-plant-based-bioeconomy/maria-cuacos_1920px.jpg/_jcr_content/renditions/original.image_file.768.432.0,234,1920,1314.file/maria-cuacos_1920px.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/breeding-research-on-the-way-to-a-plant-based-bioeconomy/maria-cuacos_1920px.jpg/_jcr_content/renditions/original.image_file.1024.576.0,234,1920,1314.file/maria-cuacos_1920px.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/breeding-research-on-the-way-to-a-plant-based-bioeconomy/maria-cuacos_1920px.jpg/_jcr_content/renditions/original.image_file.1280.720.0,234,1920,1314.file/maria-cuacos_1920px.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/breeding-research-on-the-way-to-a-plant-based-bioeconomy/maria-cuacos_1920px.jpg/_jcr_content/renditions/original.image_file.1440.810.0,234,1920,1314.file/maria-cuacos_1920px.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/breeding-research-on-the-way-to-a-plant-based-bioeconomy/maria-cuacos_1920px.jpg/_jcr_content/renditions/original.image_file.1920.1080.0,234,1920,1314.file/maria-cuacos_1920px.jpg"} "Maria Cuacos | Crop Research at IPK Gatersleben")
![ZEISS Labscope for Classroom - Create engaging lessons with your digital classroom.]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zeiss-labscope/teaching/labscope_teaching-group.jpg/_jcr_content/renditions/original.image_file.100.100.264,0,1571,1307.file/labscope_teaching-group.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zeiss-labscope/teaching/labscope_teaching-group.jpg/_jcr_content/renditions/original.image_file.360.360.264,0,1571,1307.file/labscope_teaching-group.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zeiss-labscope/teaching/labscope_teaching-group.jpg/_jcr_content/renditions/original.image_file.768.768.264,0,1571,1307.file/labscope_teaching-group.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zeiss-labscope/teaching/labscope_teaching-group.jpg/_jcr_content/renditions/original.image_file.1024.1024.264,0,1571,1307.file/labscope_teaching-group.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zeiss-labscope/teaching/labscope_teaching-group.jpg/_jcr_content/renditions/original.image_file.1280.1280.264,0,1571,1307.file/labscope_teaching-group.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zeiss-labscope/teaching/labscope_teaching-group.jpg/_jcr_content/renditions/original.image_file.1307.1307.264,0,1571,1307.file/labscope_teaching-group.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zeiss-labscope/teaching/labscope_teaching-group.jpg/_jcr_content/renditions/original.image_file.1307.1307.264,0,1571,1307.file/labscope_teaching-group.jpg"} "ZEISS Labscope for Classroom")
![ZEISS Labscope for Your Daily Laboratory Work]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zeiss-labscope/laboratory/labscope_ai-cell-count-confluency_result-table.jpg/_jcr_content/renditions/original.image_file.100.100.270,0,1650,1380.file/labscope_ai-cell-count-confluency_result-table.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zeiss-labscope/laboratory/labscope_ai-cell-count-confluency_result-table.jpg/_jcr_content/renditions/original.image_file.360.360.270,0,1650,1380.file/labscope_ai-cell-count-confluency_result-table.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zeiss-labscope/laboratory/labscope_ai-cell-count-confluency_result-table.jpg/_jcr_content/renditions/original.image_file.768.768.270,0,1650,1380.file/labscope_ai-cell-count-confluency_result-table.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zeiss-labscope/laboratory/labscope_ai-cell-count-confluency_result-table.jpg/_jcr_content/renditions/original.image_file.1024.1024.270,0,1650,1380.file/labscope_ai-cell-count-confluency_result-table.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zeiss-labscope/laboratory/labscope_ai-cell-count-confluency_result-table.jpg/_jcr_content/renditions/original.image_file.1280.1280.270,0,1650,1380.file/labscope_ai-cell-count-confluency_result-table.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zeiss-labscope/laboratory/labscope_ai-cell-count-confluency_result-table.jpg/_jcr_content/renditions/original.image_file.1380.1380.270,0,1650,1380.file/labscope_ai-cell-count-confluency_result-table.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zeiss-labscope/laboratory/labscope_ai-cell-count-confluency_result-table.jpg/_jcr_content/renditions/original.image_file.1380.1380.270,0,1650,1380.file/labscope_ai-cell-count-confluency_result-table.jpg"} "ZEISS Labscope for Your Daily Laboratory Work")