Webinar
3D SEM
Large Volume Electron Microscopy Enabling Statistical Analysis
10 February 2021
· 60 min watch
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/authors/joel_mancuso.jpg/_jcr_content/renditions/original.image_file.100.100.0,1,389,390.file/joel_mancuso.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/authors/joel_mancuso.jpg/_jcr_content/renditions/original.image_file.360.360.0,1,389,390.file/joel_mancuso.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/authors/joel_mancuso.jpg/_jcr_content/renditions/original.image_file.389.389.0,1,389,390.file/joel_mancuso.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/authors/joel_mancuso.jpg/_jcr_content/renditions/original.image_file.389.389.0,1,389,390.file/joel_mancuso.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/authors/joel_mancuso.jpg/_jcr_content/renditions/original.image_file.389.389.0,1,389,390.file/joel_mancuso.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/authors/joel_mancuso.jpg/_jcr_content/renditions/original.image_file.389.389.0,1,389,390.file/joel_mancuso.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/authors/joel_mancuso.jpg/_jcr_content/renditions/original.image_file.389.389.0,1,389,390.file/joel_mancuso.jpg"})
Author
Joel Mancuso
Life Science EM XRM Product Marketing Manager
ZEISS Microscopy
ZEISS Microscopy
Abstract
3DSEM refers to a group of techniques and products that acquires serial 2d images to create a 3D volume. This talk explains the strength and weakness of each of the techniques to help guide you in choosing the best solution for your applications. It also introduces some recent advancements in automating sample prep and image segmentation to help generate meaningful statistics from 3DSEM data.
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/thumbnails/screensaver_3d-sem.jpg/_jcr_content/renditions/original.image_file.100.56.0,9,1755,996.file/screensaver_3d-sem.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/thumbnails/screensaver_3d-sem.jpg/_jcr_content/renditions/original.image_file.360.203.0,9,1755,996.file/screensaver_3d-sem.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/thumbnails/screensaver_3d-sem.jpg/_jcr_content/renditions/original.image_file.768.432.0,9,1755,996.file/screensaver_3d-sem.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/thumbnails/screensaver_3d-sem.jpg/_jcr_content/renditions/original.image_file.1024.576.0,9,1755,996.file/screensaver_3d-sem.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/thumbnails/screensaver_3d-sem.jpg/_jcr_content/renditions/original.image_file.1280.720.0,9,1755,996.file/screensaver_3d-sem.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/thumbnails/screensaver_3d-sem.jpg/_jcr_content/renditions/original.image_file.1440.810.0,9,1755,996.file/screensaver_3d-sem.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/thumbnails/screensaver_3d-sem.jpg/_jcr_content/renditions/original.image_file.1755.987.0,9,1755,996.file/screensaver_3d-sem.jpg"})
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/cacao-pollination-the-sweet-science-a-study-of-floral-morphology-using-3d-imaging/3d-pollination_versa-specimen-chamber-loading.jpg/_jcr_content/renditions/original.image_file.100.56.0,359,3030,2063.file/3d-pollination_versa-specimen-chamber-loading.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/cacao-pollination-the-sweet-science-a-study-of-floral-morphology-using-3d-imaging/3d-pollination_versa-specimen-chamber-loading.jpg/_jcr_content/renditions/original.image_file.360.203.0,359,3030,2063.file/3d-pollination_versa-specimen-chamber-loading.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/cacao-pollination-the-sweet-science-a-study-of-floral-morphology-using-3d-imaging/3d-pollination_versa-specimen-chamber-loading.jpg/_jcr_content/renditions/original.image_file.768.432.0,359,3030,2063.file/3d-pollination_versa-specimen-chamber-loading.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/cacao-pollination-the-sweet-science-a-study-of-floral-morphology-using-3d-imaging/3d-pollination_versa-specimen-chamber-loading.jpg/_jcr_content/renditions/original.image_file.1024.576.0,359,3030,2063.file/3d-pollination_versa-specimen-chamber-loading.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/cacao-pollination-the-sweet-science-a-study-of-floral-morphology-using-3d-imaging/3d-pollination_versa-specimen-chamber-loading.jpg/_jcr_content/renditions/original.image_file.1280.720.0,359,3030,2063.file/3d-pollination_versa-specimen-chamber-loading.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/cacao-pollination-the-sweet-science-a-study-of-floral-morphology-using-3d-imaging/3d-pollination_versa-specimen-chamber-loading.jpg/_jcr_content/renditions/original.image_file.1440.810.0,359,3030,2063.file/3d-pollination_versa-specimen-chamber-loading.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/cacao-pollination-the-sweet-science-a-study-of-floral-morphology-using-3d-imaging/3d-pollination_versa-specimen-chamber-loading.jpg/_jcr_content/renditions/original.image_file.1920.1080.0,359,3030,2063.file/3d-pollination_versa-specimen-chamber-loading.jpg"})
![Unlocking Scientific Discoveries at a Core Imaging Facility: Gaining Perspective from the Champalimaud Foundation in Lisbon]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/unlocking-scientific-discoveries-at-a-core-imaging-facility-gaining-perspective-from-the-champalimaud-foundation-in-lisbon/banner_platform_advanced-bioimaging-and-biooptics-experimental-platform.jpg/_jcr_content/renditions/original.image_file.100.56.3,0,1368,768.file/banner_platform_advanced-bioimaging-and-biooptics-experimental-platform.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/unlocking-scientific-discoveries-at-a-core-imaging-facility-gaining-perspective-from-the-champalimaud-foundation-in-lisbon/banner_platform_advanced-bioimaging-and-biooptics-experimental-platform.jpg/_jcr_content/renditions/original.image_file.360.203.3,0,1368,768.file/banner_platform_advanced-bioimaging-and-biooptics-experimental-platform.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/unlocking-scientific-discoveries-at-a-core-imaging-facility-gaining-perspective-from-the-champalimaud-foundation-in-lisbon/banner_platform_advanced-bioimaging-and-biooptics-experimental-platform.jpg/_jcr_content/renditions/original.image_file.768.432.3,0,1368,768.file/banner_platform_advanced-bioimaging-and-biooptics-experimental-platform.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/unlocking-scientific-discoveries-at-a-core-imaging-facility-gaining-perspective-from-the-champalimaud-foundation-in-lisbon/banner_platform_advanced-bioimaging-and-biooptics-experimental-platform.jpg/_jcr_content/renditions/original.image_file.1024.576.3,0,1368,768.file/banner_platform_advanced-bioimaging-and-biooptics-experimental-platform.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/unlocking-scientific-discoveries-at-a-core-imaging-facility-gaining-perspective-from-the-champalimaud-foundation-in-lisbon/banner_platform_advanced-bioimaging-and-biooptics-experimental-platform.jpg/_jcr_content/renditions/original.image_file.1280.720.3,0,1368,768.file/banner_platform_advanced-bioimaging-and-biooptics-experimental-platform.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/unlocking-scientific-discoveries-at-a-core-imaging-facility-gaining-perspective-from-the-champalimaud-foundation-in-lisbon/banner_platform_advanced-bioimaging-and-biooptics-experimental-platform.jpg/_jcr_content/renditions/original.image_file.1365.768.3,0,1368,768.file/banner_platform_advanced-bioimaging-and-biooptics-experimental-platform.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/unlocking-scientific-discoveries-at-a-core-imaging-facility-gaining-perspective-from-the-champalimaud-foundation-in-lisbon/banner_platform_advanced-bioimaging-and-biooptics-experimental-platform.jpg/_jcr_content/renditions/original.image_file.1365.768.3,0,1368,768.file/banner_platform_advanced-bioimaging-and-biooptics-experimental-platform.jpg"} "Unlocking Scientific Discoveries at a Core Imaging Facility: Gaining Perspective from the Champalimaud Foundation in Lisbon")
![A scientist wearing a lab coat uses a pipette in a laboratory filled with equipment and supplies.]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/unveiling-the-secrets-of-a-seawater-drop-exploring-europes-seas-and-coasts/trec-naples.jpg/_jcr_content/renditions/original.image_file.100.56.0,261,5000,3074.file/trec-naples.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/unveiling-the-secrets-of-a-seawater-drop-exploring-europes-seas-and-coasts/trec-naples.jpg/_jcr_content/renditions/original.image_file.360.203.0,261,5000,3074.file/trec-naples.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/unveiling-the-secrets-of-a-seawater-drop-exploring-europes-seas-and-coasts/trec-naples.jpg/_jcr_content/renditions/original.image_file.768.432.0,261,5000,3074.file/trec-naples.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/unveiling-the-secrets-of-a-seawater-drop-exploring-europes-seas-and-coasts/trec-naples.jpg/_jcr_content/renditions/original.image_file.1024.576.0,261,5000,3074.file/trec-naples.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/unveiling-the-secrets-of-a-seawater-drop-exploring-europes-seas-and-coasts/trec-naples.jpg/_jcr_content/renditions/original.image_file.1280.720.0,261,5000,3074.file/trec-naples.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/unveiling-the-secrets-of-a-seawater-drop-exploring-europes-seas-and-coasts/trec-naples.jpg/_jcr_content/renditions/original.image_file.1440.810.0,261,5000,3074.file/trec-naples.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/resource-center/insights-hub/user-story/unveiling-the-secrets-of-a-seawater-drop-exploring-europes-seas-and-coasts/trec-naples.jpg/_jcr_content/renditions/original.image_file.1920.1080.0,261,5000,3074.file/trec-naples.jpg"} "Unveiling the secrets of a seawater drop: exploring Europe's seas and coasts")
![ZEISS Crossbeam - FIB-SEM for High Throughput 3D Analysis and Sample Preparation]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.100.100.file/crossbeam-550-system.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.360.360.file/crossbeam-550-system.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.768.768.file/crossbeam-550-system.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/crossbeam-550-system.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/crossbeam-550-system.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/crossbeam-550-system.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original./crossbeam-550-system.jpg"} "ZEISS Crossbeam")
![ZEISS Crossbeam 550 Samplefab]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam-samplefab/crossbeam-550-samplefab_w-monitor_right-side.png/_jcr_content/renditions/original.image_file.100.100.file/crossbeam-550-samplefab_w-monitor_right-side.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam-samplefab/crossbeam-550-samplefab_w-monitor_right-side.png/_jcr_content/renditions/original.image_file.360.360.file/crossbeam-550-samplefab_w-monitor_right-side.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam-samplefab/crossbeam-550-samplefab_w-monitor_right-side.png/_jcr_content/renditions/original.image_file.768.768.file/crossbeam-550-samplefab_w-monitor_right-side.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam-samplefab/crossbeam-550-samplefab_w-monitor_right-side.png/_jcr_content/renditions/original.image_file.1024.1024.file/crossbeam-550-samplefab_w-monitor_right-side.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam-samplefab/crossbeam-550-samplefab_w-monitor_right-side.png/_jcr_content/renditions/original.image_file.1280.1280.file/crossbeam-550-samplefab_w-monitor_right-side.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam-samplefab/crossbeam-550-samplefab_w-monitor_right-side.png/_jcr_content/renditions/original.image_file.1440.1440.file/crossbeam-550-samplefab_w-monitor_right-side.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam-samplefab/crossbeam-550-samplefab_w-monitor_right-side.png/_jcr_content/renditions/original.image_file.1920.1920.file/crossbeam-550-samplefab_w-monitor_right-side.png"} "ZEISS Crossbeam 550 Samplefab")
![ZEISS GeminiSEM Family]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.100.100.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.360.360.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.768.768.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.1024.1024.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.1280.1280.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.1353.1353.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.1353.1353.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg"} "ZEISS GeminiSEM Family")
![ZEISS MultiSEM: The World’s Fastest Scanning Electron Microscope]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/multisem/multisem-stage.jpg/_jcr_content/renditions/original.image_file.100.100.420,0,1500,1080.file/multisem-stage.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/multisem/multisem-stage.jpg/_jcr_content/renditions/original.image_file.360.360.420,0,1500,1080.file/multisem-stage.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/multisem/multisem-stage.jpg/_jcr_content/renditions/original.image_file.768.768.420,0,1500,1080.file/multisem-stage.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/multisem/multisem-stage.jpg/_jcr_content/renditions/original.image_file.1024.1024.420,0,1500,1080.file/multisem-stage.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/multisem/multisem-stage.jpg/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/multisem-stage.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/multisem/multisem-stage.jpg/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/multisem-stage.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/multisem/multisem-stage.jpg/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/multisem-stage.jpg"} "ZEISS MultiSEM: The World’s Fastest Scanning Electron Microscope")
![ZEISS Sigma]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.100.100.420,0,1500,1080.file/sigma360-stage.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.360.360.420,0,1500,1080.file/sigma360-stage.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.768.768.420,0,1500,1080.file/sigma360-stage.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.1024.1024.420,0,1500,1080.file/sigma360-stage.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/sigma360-stage.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/sigma360-stage.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/sigma360-stage.png"} "ZEISS Sigma")