Episode 3: Array Tomography - A Flexible and Accessible Volume-EM Technique

Intricate 3D ultrastructural information can be delivered by SEM technologies and methods, known collectively as Volume EM (vEM). vEM drives new discoveries in neurobiology, cancer research, developmental biology, plant science, and more. This six-part webinar series will explore the technological foundations of vEM imaging and highlight the growing number of application areas.

Array tomography is a volume EM technique where serial ultra-thin sections are cut and collected onto a substrate, and then imaged in an SEM to build up a 3D representation of that sample. Due to the non-destructive nature of the technique, and the stability of the sections on substrate, arrays are amenable to repeated sessions of automated image acquisition, providing unlimited new regions of interest according to the user’s needs and data handling capabilities.

Key Learnings:

• Learn about the Array Tomography (AT) workflow and why AT is a genuinely accessible vEM technique with standard equipment that many EM labs will already have
• Get to know its practical benefits to biomedical and clinical researchers, core facilities and community groups
• Get tips and tricks that could help you start accessing the benefits of Array Tomography for your research
• Get a researcher’s perspective on how to sample liver tissue for the tracing and reconstruction of hepatic nerve endings