Episode 5: Exploring Cellular Structures with FIB-SEM Isotropic Nanoscale Imaging

Intricate 3D ultrastructural information can be delivered by SEM technologies and methods, known collectively as Volume EM (vEM). vEM drives new discoveries in neurobiology, cancer research, developmental biology, plant science, and more. This six-part webinar series will explore the technological foundations of vEM imaging and highlight the growing number of application areas.

Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) stands out as a stellar technology that provides unprecedented insights into the three-dimensional morphology of cells with isotropic nanoscale resolution. In this webinar, Ilya Belevich will discuss the principles and applications of FIB-SEM within cell biology.

Key Learnings:

• Introduction to the fundamental concepts of FIB-SEM
• The necessary criteria for sample preparation
• Cell biology case studies: How FIB-SEM can be paired with other imaging techniques such as confocal light microscopy and serial block-face SEM to broaden our perspective through correlative approaches
• How to tackle the complexities of data processing: Strategies to convert qualitative insights into quantitatively robust, visually compelling findings