Frequently Asked Questions About Imaging Cleared Tissues

This presentation addresses five frequently asked questions that typically arise while preparing and imaging optically cleared tissue samples with Lightsheet and other microscopes:

1. Why do I need to clear my sample?
2. What clearing method should I use?
3. How do I image my sample after clearing?
4. How can I improve my image quality?
5. How do I manage all the data?

The information presented here is intended to serve as an introductory overview for researchers that are just getting into tissue clearing, as well as a reference for more advanced individuals that want to optimize imaging of their already cleared samples.