The famous Cambridge moonflower before ultrastructural studies with scanning electron microscopy
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Rare Moonflower Captured with Scanning Electron Microscopy

Plant scientists explore the cellular ultrastructure in tepals, leaves, stem, and pollen of the rare and famous Cambridge moonflower.

Plant researchers and local enthusiasts in the United Kingdom and beyond were excited to discover rare blooms on their moonflower, Strophocactus wittii, at Cambridge University Botanic Garden. The cactus first made headlines during quarantine in 2021 during its famous first bloom over live stream. While common in the Amazon forests, these moonflowers are uncommon and difficult to nurture in gardens with plant blooms occurring less than once per year. Additionally, the flowers last less than twelve hours, which gives plant scientists very little time to collect data to learn more about these elusive structures.

Fortunately, microscopists at the imaging core facility, housed within the grounds of the garden at the Sainsbury Laboratory Cambridge University (SLCU), were ready for when the flowers bloomed. Dr. Raymond Wightman is the Core Facility Manager at SLCU and led the project to capture images of this delicate specimen using cryo scanning electron microscopy with the ZEISS EVO SEM. He worked with Kristina Buch, a PhD student in Plant Sciences at the University of Cambridge. Their work documents the first ultrastructural images of different moonflower tissues and identified an unusual tissue structure they look forward to studying with the next rare flowering.

Dr. Raymond Wightman with ZEISS EVO Scanning Electron Microscope

Given the moonflower opens very rarely, our aim was to do cryo scanning electron microscopy (cryoSEM) and collect a permanent record of plant tissue organization and cellular ultrastructure. 

Dr. Raymond Wightman Core Facility Manager, Sainsbury Laboratory Cambridge University, United Kingdom

First Cellular Ultrastructure Images of the Moonflower Plant

Captured with ZEISS EVO Scanning Electron Microscopy (SEM)

First cellular ultrastructure images of moonflower plant using scanning electron microscopy
First cellular ultrastructure images of moonflower plant using scanning electron microscopy

First images taken by cryoSEM of the moonflower plant. Left: pollen. Right upper and center: cryofracture of a tepal. Right lower: cryofracture of a stem. Images collected by Kristina Buch with the ZEISS EVO scanning electron microscope (SEM).

First images taken by cryoSEM of the moonflower plant. Left: pollen. Right upper and center: cryofracture of a tepal. Right lower: cryofracture of a stem. Images collected by Kristina Buch with the ZEISS EVO scanning electron microscope (SEM).

SEM Ultrastructural Data of a Transient Plant Bloom

As this was the first time working with the moonflower in the electron microscopy facility, the focus was basic characterization of cellular ultrastructure to understand how cells are organized within the tissue. They collected data from the pollen, leaf and stem area, as well as the tepals - these are the so-called "petals" as it is not entirely understood which structures represent sepals or petals in the normal moonflower anatomy.

While they are still early in analyzing all the data, they've already noticed one feature that is unusual. However, the team will need to take more images the next time a flower opens.

Kristina Buch with Dr. Raymond Wightman at their ZEISS EVO scanning electron microscope

These initial studies on plant tissue organization and cell ultrastructure help us determine which other routes of experimentation may be most promising to pursue with the next rare flowering.

Kristina Buch PhD Student in Plant Sciences, University of Cambridge, United Kingdom

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