How-to Video
How to Set up Koehler Illumination
On Your Upright Transmitted Light Microscope
8 April 2020
· 3 min watch
Abstract
How to Set up Koehler Illumination: On Your Upright Transmitted Light Microscope
You want to image your samples with optimized contrast and resolution?
Then you need to apply Köhler illumination, a process that provides homogeneous illumination without stray light. It still is the only method to obtain reproducible quantitative data in light microscopy.