![Cerveau de souris coloré avec un colorant lipidique pour marquer les membranes vasculaires, transparisées avec iDISCO+ et capturées avec la microscopie à feuillet de lumière. Échantillon avec l'aimable autorisation de E. Diel, Université d'Harvard, États-Unis]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-vasculature.jpg/_jcr_content/renditions/original.image_file.100.100.164,185,1714,1735.file/brain-vasculature.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-vasculature.jpg/_jcr_content/renditions/original.image_file.360.360.164,185,1714,1735.file/brain-vasculature.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-vasculature.jpg/_jcr_content/renditions/original.image_file.768.768.164,185,1714,1735.file/brain-vasculature.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-vasculature.jpg/_jcr_content/renditions/original.image_file.1024.1024.164,185,1714,1735.file/brain-vasculature.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-vasculature.jpg/_jcr_content/renditions/original.image_file.1280.1280.164,185,1714,1735.file/brain-vasculature.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-vasculature.jpg/_jcr_content/renditions/original.image_file.1440.1440.164,185,1714,1735.file/brain-vasculature.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-vasculature.jpg/_jcr_content/renditions/original.image_file.1550.1550.164,185,1714,1735.file/brain-vasculature.jpg"} "Cerveau de souris coloré avec un colorant lipidique pour marquer les membranes vasculaires, transparisées avec iDISCO+ et capturées avec la microscopie à feuillet de lumière. Échantillon avec l'aimable autorisation de E. Diel, Université d'Harvard, États-Unis")
Cerveau et tissus épais transparisés par procédé chimique
Examinez en profondeur vos échantillons
Avec l'essor des techniques de compensation optique des tissus, des échantillons volumineux tels que des cerveaux entiers, des organes ou de grands organismes modèles peuvent être examinés à une résolution cellulaire ou subcellulaire sans pour autant recourir au sectionnement physique habituel. Ce développement est essentiel pour les chercheurs qui étudient l'anatomie, la vascularisation, les réseaux de neurones ou la connectomique.
Les méthodes de compensation optique, associées à la puissance informatique, à la capacité de stockage des données, aux algorithmes intelligents de rendu et d'analyse et à la technologie adéquate de microscopie, permettent une analyse efficace de ces grands échantillons.
![Rein de souris transparisé avec le protocole iDISCO, image capturée par microscopie à feuillet de lumière. Échantillon avec l'aimable autorisation d'U. Roostalu, Gubra, Danemark.]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.100.67.0,0,1917,1280.file/mouse-kidney-eci-gubra-panel.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.360.240.0,0,1917,1280.file/mouse-kidney-eci-gubra-panel.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.768.512.0,0,1917,1280.file/mouse-kidney-eci-gubra-panel.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.1024.683.0,0,1917,1280.file/mouse-kidney-eci-gubra-panel.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.1280.853.0,0,1917,1280.file/mouse-kidney-eci-gubra-panel.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.1440.960.0,0,1917,1280.file/mouse-kidney-eci-gubra-panel.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.1917.1278.0,0,1917,1280.file/mouse-kidney-eci-gubra-panel.jpg"} "Rein de souris transparisé avec le protocole iDISCO, image capturée par microscopie à feuillet de lumière. Échantillon avec l'aimable autorisation d'U. Roostalu, Gubra, Danemark.")
![Rein de souris transparisé avec le protocole iDISCO, image capturée par microscopie à feuillet de lumière. Échantillon avec l'aimable autorisation d'U. Roostalu, Gubra, Danemark.]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.100.67.file/mouse-kidney-eci-gubra-panel.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.360.240.file/mouse-kidney-eci-gubra-panel.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.768.512.file/mouse-kidney-eci-gubra-panel.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.1024.683.file/mouse-kidney-eci-gubra-panel.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.1280.853.file/mouse-kidney-eci-gubra-panel.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.1440.960.file/mouse-kidney-eci-gubra-panel.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original./mouse-kidney-eci-gubra-panel.jpg"} "Rein de souris transparisé avec le protocole iDISCO, image capturée par microscopie à feuillet de lumière. Échantillon avec l'aimable autorisation d'U. Roostalu, Gubra, Danemark.")
Rein de souris transparisé avec le protocole iDISCO, image capturée par microscopie à feuillet de lumière. Échantillon avec l'aimable autorisation d'U. Roostalu, Gubra, Danemark.
Rein de souris transparisé avec le protocole iDISCO, image capturée par microscopie à feuillet de lumière. Échantillon avec l'aimable autorisation d'U. Roostalu, Gubra, Danemark.
La microscopie à feuillet de lumière offre un équilibre parfait entre une grande taille d'échantillon, une imagerie haute résolution et une vitesse d'acquisition élevée
La microscopie en fluorescence à feuillet de lumière (LSFM) utilise une méthode d'éclairage unique pour exciter et collecter des plans entiers de données en une fois. L'utilisation d'échantillons transparisés permet de collecter des données en haute résolution sur un échantillon de grande taille à des vitesses extrêmement élevées. ZEISS Lightsheet 7 est l'équipement idéal pour réaliser une imagerie rapide de cerveaux, d'organes ou de grandes sections de tissus transparisés par procédé chimique.
![Cerveau de souris transparisé à l'aide du protocole CLARITY]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.100.67.0,0,1591,1062.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.360.240.0,0,1591,1062.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.768.512.0,0,1591,1062.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.1024.683.0,0,1591,1062.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.1280.853.0,0,1591,1062.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.1440.960.0,0,1591,1062.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.1591.1061.0,0,1591,1062.file/clearing_mouse-neurons_axio-examiner_lsm.jpg"} "Cerveau de souris transparisé à l'aide du protocole CLARITY")
![Cerveau de souris transparisé à l'aide du protocole CLARITY]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.100.67.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.360.240.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.768.512.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.1024.683.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.1280.853.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.1440.960.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original./clearing_mouse-neurons_axio-examiner_lsm.jpg"} "Cerveau de souris transparisé à l'aide du protocole CLARITY")
Cerveau de souris transparisé à l'aide du protocole CLARITY, image capturée par microscopie confocale. Échantillon avec l'aimable autorisation de T. Ruff, Institut Max Planck de neurobiologie, Allemagne.
Cerveau de souris transparisé à l'aide du protocole CLARITY, image capturée par microscopie confocale. Échantillon avec l'aimable autorisation de T. Ruff, Institut Max Planck de neurobiologie, Allemagne.
La microscopie confocale : obtenez une résolution et des détails structurels incroyables dans des échantillons transparisés
La microscopie confocale révèle des détails subcellulaires infimes et des informations structurelles en haute résolution provenant des profondeurs des cerveaux, des organes ou d'autres tissus transparisés par procédé chimique. La gamme confocale ZEISS LSM 9 avec Airyscan est équipée de capacités de super résolution et d'un mode Multiplex pour des vitesses d'acquisition améliorées afin de capturer rapidement les structures les plus fines.
![Cerveau de souris transparisé. En haut à droite : Aperçu du cerveau entier.]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.100.67.0,0,945,631.file/brain-erturk.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.360.240.0,0,945,631.file/brain-erturk.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.768.512.0,0,945,631.file/brain-erturk.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.945.630.0,0,945,631.file/brain-erturk.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.945.630.0,0,945,631.file/brain-erturk.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.945.630.0,0,945,631.file/brain-erturk.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.945.630.0,0,945,631.file/brain-erturk.jpg"} "Cerveau de souris transparisé. En haut à droite : Aperçu du cerveau entier.")
![Cerveau de souris transparisé. En haut à droite : Aperçu du cerveau entier.]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.100.67.file/brain-erturk.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.360.240.file/brain-erturk.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.768.512.file/brain-erturk.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original./brain-erturk.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original./brain-erturk.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original./brain-erturk.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original./brain-erturk.jpg"} "Cerveau de souris transparisé. En haut à droite : Aperçu du cerveau entier.")
Cerveau de souris transparisé. En haut à droite : Aperçu du cerveau entier. À gauche : Projection à intensité maximale (PIM) d'une région cérébrale. En bas à droite : Détail d'un encadré PIM. Acquis par microscopie à zoom et éclairage structuré pour le sectionnement optique. Échantillon avec l'aimable autorisation de Erturk lab, Université Ludwig Maximilian de Munich, Allemagne
Cerveau de souris transparisé. En haut à droite : Aperçu du cerveau entier. À gauche : Projection à intensité maximale (PIM) d'une région cérébrale. En bas à droite : Détail d'un encadré PIM. Acquis par microscopie à zoom et éclairage structuré pour le sectionnement optique. Échantillon avec l'aimable autorisation de Erturk lab, Université Ludwig Maximilian de Munich, Allemagne
Imagerie à large champ d'observation avec une résolution cellulaire
La microscopie à zoom avec un cerveau transparisé par procédé chimique ou de grands échantillons de tissus permet une résolution cellulaire dans de très larges champs d'observation. Le microscope à zoom ZEISS Axio Zoom.V16 peut fournir une grande image d'ensemble, puis permet de zoomer et d'observer des cellules individuelles dans les tissus transparisés. Équipez Axio Zoom.V16 de ZEISS Apotome 3 pour ajouter une capacité de sectionnement optique et des informations structurelles améliorées.
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