![Array Tomography]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography.jpg/_jcr_content/renditions/original.image_file.100.56.file/array-tomography.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography.jpg/_jcr_content/renditions/original.image_file.360.203.file/array-tomography.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography.jpg/_jcr_content/renditions/original.image_file.768.432.file/array-tomography.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography.jpg/_jcr_content/renditions/original.image_file.1024.576.file/array-tomography.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography.jpg/_jcr_content/renditions/original.image_file.1280.720.file/array-tomography.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography.jpg/_jcr_content/renditions/original.image_file.1440.810.file/array-tomography.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography.jpg/_jcr_content/renditions/original./array-tomography.jpg"} "Array Tomography")
Array Tomography
Imagerie de volume non destructive avec microscope électronique à balayage standard
Représentation schématique d'un flux de tâches typique
![Préparation des échantillons]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography-sample-preparation.png/_jcr_content/renditions/original.image_file.100.100.file/array-tomography-sample-preparation.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography-sample-preparation.png/_jcr_content/renditions/original.image_file.360.360.file/array-tomography-sample-preparation.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography-sample-preparation.png/_jcr_content/renditions/original.image_file.768.768.file/array-tomography-sample-preparation.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography-sample-preparation.png/_jcr_content/renditions/original.image_file.1024.1024.file/array-tomography-sample-preparation.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography-sample-preparation.png/_jcr_content/renditions/original./array-tomography-sample-preparation.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography-sample-preparation.png/_jcr_content/renditions/original./array-tomography-sample-preparation.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography-sample-preparation.png/_jcr_content/renditions/original./array-tomography-sample-preparation.png"} "Préparation des échantillons")
1
Un échantillon enrobé de résine est découpé en un ensemble de coupes sériées, chacune d'une épaisseur de 30 à 70 nm, et fixé à un porte-échantillon dans l'ordre où les coupes ont été réalisées.
![Acquisition d'image]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography-image-acquisition.png/_jcr_content/renditions/original.image_file.100.100.file/array-tomography-image-acquisition.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography-image-acquisition.png/_jcr_content/renditions/original.image_file.360.360.file/array-tomography-image-acquisition.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography-image-acquisition.png/_jcr_content/renditions/original.image_file.768.768.file/array-tomography-image-acquisition.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography-image-acquisition.png/_jcr_content/renditions/original.image_file.1024.1024.file/array-tomography-image-acquisition.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography-image-acquisition.png/_jcr_content/renditions/original./array-tomography-image-acquisition.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography-image-acquisition.png/_jcr_content/renditions/original./array-tomography-image-acquisition.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/array-tomography-image-acquisition.png/_jcr_content/renditions/original./array-tomography-image-acquisition.png"} "Acquisition d'image")
2
Chaque coupe sériée est imagée au microscope électronique à balayage (MEB).
![Traitement de la segmentation]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-processing-segmentation.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-processing-segmentation.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-processing-segmentation.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-processing-segmentation.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png"} "Traitement de la segmentation")
3
Les images acquises au microscope électronique sont traitées et alignées numériquement dans un ensemble de données 3D. Les compartiments cellulaires peuvent être identifiés et segmentés.
![Analyse de la visualisation en 3D]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-3d-visualization-analysis.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-3d-visualization-analysis.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-3d-visualization-analysis.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-3d-visualization-analysis.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png"} "Analyse de la visualisation en 3D")
4
L'ensemble des données 3D segmentées peut être visualisé, étudié et analysé statistiquement.
Exemple d'application
Reconstruction 3D de coupes sériées de nodules racinaires avec la distribution des plasmodesmes
![La relation symbiotique entre les plantes et les bactéries]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/root-nodules-correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.100.100.file/root-nodules-correlative-array-tomography.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/root-nodules-correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.360.360.file/root-nodules-correlative-array-tomography.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/root-nodules-correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.768.768.file/root-nodules-correlative-array-tomography.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/root-nodules-correlative-array-tomography.jpg/_jcr_content/renditions/original./root-nodules-correlative-array-tomography.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/root-nodules-correlative-array-tomography.jpg/_jcr_content/renditions/original./root-nodules-correlative-array-tomography.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/root-nodules-correlative-array-tomography.jpg/_jcr_content/renditions/original./root-nodules-correlative-array-tomography.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/root-nodules-correlative-array-tomography.jpg/_jcr_content/renditions/original./root-nodules-correlative-array-tomography.jpg"} "La relation symbiotique entre les plantes et les bactéries")
Avec l'aimable autorisation de D. Sherrier, J. Caplan et S. Modla, Université du Delaware, États-Unis.
La relation symbiotique entre les plantes et les bactéries
Comprendre l'impact des bactéries présentes dans les nodules racinaires sur la santé et l'état des plantes
Grâce à son réseau racinaire, une plante peut accéder à toute l'eau et à tous les nutriments cruciaux pour sa croissance. Pour optimiser la santé et le rendement des plantes, il est donc important d'explorer l'ensemble du réseau racinaire et de comprendre l'influence des microbes externes. L'étude de la relation symbiotique entre les plantes et les bactéries dans les nodules racinaires impose de connaitre la distribution des nodules racinaires et des bactéries. De plus, il est essentiel d'associer la fluorescence et l'évaluation structurelle à haute résolution pour comprendre leurs interactions en détail.
La tomographie de réseau corrélative permet de superposer les données de fluorescence et les données structurelles afin de visualiser la distribution des nodules racinaires et des bactéries.
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography.jpg/_jcr_content/renditions/original.image_file.100.56.0,0,1449,815.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography.jpg/_jcr_content/renditions/original.image_file.360.203.0,0,1449,815.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography.jpg/_jcr_content/renditions/original.image_file.768.432.0,0,1449,815.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography.jpg/_jcr_content/renditions/original.image_file.1024.576.0,0,1449,815.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography.jpg/_jcr_content/renditions/original.image_file.1280.720.0,0,1449,815.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography.jpg/_jcr_content/renditions/original.image_file.1440.810.0,0,1449,815.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography.jpg/_jcr_content/renditions/original.image_file.1449.815.0,0,1449,815.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography.jpg"})
![Moniteur de bureau présentant le logiciel ZEISS arivis Pro qui affiche les résultats de l'analyse des complexes de pores nucléaires provenant des données obtenues par microscopie électronique volumétrique FIB-SEM.]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.100.100.file/zeiss-arivis-pro-product-0724.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.360.360.file/zeiss-arivis-pro-product-0724.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.768.768.file/zeiss-arivis-pro-product-0724.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss-arivis-pro-product-0724.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original./zeiss-arivis-pro-product-0724.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original./zeiss-arivis-pro-product-0724.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original./zeiss-arivis-pro-product-0724.png"} "Logiciel ZEISS arivis Pro")
![Série ZEISS GeminiSEM]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.100.100.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.360.360.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.768.768.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.1024.1024.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.1280.1280.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.1353.1353.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.1353.1353.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg"} "Série ZEISS GeminiSEM")
![ZEISS Sense BSD - Détecteur d'électrons rétrodiffusés pour l'imagerie ultrastructurale rapide et douce d'échantillons biologiques]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-metadata.jpg/_jcr_content/renditions/original.image_file.100.100.506,29,1522,1045.file/sensebsd_product-metadata.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-metadata.jpg/_jcr_content/renditions/original.image_file.360.360.506,29,1522,1045.file/sensebsd_product-metadata.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-metadata.jpg/_jcr_content/renditions/original.image_file.768.768.506,29,1522,1045.file/sensebsd_product-metadata.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-metadata.jpg/_jcr_content/renditions/original.image_file.1016.1016.506,29,1522,1045.file/sensebsd_product-metadata.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-metadata.jpg/_jcr_content/renditions/original.image_file.1016.1016.506,29,1522,1045.file/sensebsd_product-metadata.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-metadata.jpg/_jcr_content/renditions/original.image_file.1016.1016.506,29,1522,1045.file/sensebsd_product-metadata.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-metadata.jpg/_jcr_content/renditions/original.image_file.1016.1016.506,29,1522,1045.file/sensebsd_product-metadata.jpg"} "ZEISS Sense BSD")
![ZEISS Sigma]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.100.100.420,0,1500,1080.file/sigma360-stage.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.360.360.420,0,1500,1080.file/sigma360-stage.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.768.768.420,0,1500,1080.file/sigma360-stage.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.1024.1024.420,0,1500,1080.file/sigma360-stage.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/sigma360-stage.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/sigma360-stage.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/sigma360-stage.png"} "ZEISS Sigma")
![ZEN Correlative Array Tomography]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/ZEN%20Correlative%20Array%20Tomography/correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.100.100.file/correlative-array-tomography.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/ZEN%20Correlative%20Array%20Tomography/correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.360.360.file/correlative-array-tomography.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/ZEN%20Correlative%20Array%20Tomography/correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.768.768.file/correlative-array-tomography.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/ZEN%20Correlative%20Array%20Tomography/correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/correlative-array-tomography.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/ZEN%20Correlative%20Array%20Tomography/correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/correlative-array-tomography.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/ZEN%20Correlative%20Array%20Tomography/correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/correlative-array-tomography.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/ZEN%20Correlative%20Array%20Tomography/correlative-array-tomography.jpg/_jcr_content/renditions/original./correlative-array-tomography.jpg"} "ZEN Correlative Array Tomography")