![Microscopie électronique volumétrique avec MEB à faisceau d'ions focalisés Microscopie électronique volumétrique avec MEB à faisceau d'ions focalisés]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.100.56.file/fib-sem.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.360.203.file/fib-sem.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.768.432.file/fib-sem.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.1024.576.file/fib-sem.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.1280.720.file/fib-sem.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.1440.810.file/fib-sem.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original./fib-sem.jpg"})
Microscopes électroniques à balayage et à faisceau d'ions focalisé
Données volumétriques isotropes à haute résolution pour des reconstructions 3D précises
Représentation schématique d'un flux de tâches typique
![Broyage FIB-SEM Broyage FIB-SEM]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.100.100.0,1,1280,1281.file/fib-sem-milling.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.360.360.0,1,1280,1281.file/fib-sem-milling.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.768.768.0,1,1280,1281.file/fib-sem-milling.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.1024.1024.0,1,1280,1281.file/fib-sem-milling.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-milling.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-milling.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-milling.png"})
1
Une tranche est broyée dans un échantillon enrobé de résine à l'aide d'un faisceau d'ions focalisé jusqu'à ce que la structure d'intérêt devienne visible.
![Acquisition d'image FIN-SEM Acquisition d'image FIN-SEM]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.100.100.0,1,1280,1281.file/fib-sem-image-acquisition.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.360.360.0,1,1280,1281.file/fib-sem-image-acquisition.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.768.768.0,1,1280,1281.file/fib-sem-image-acquisition.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1024.1024.0,1,1280,1281.file/fib-sem-image-acquisition.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-image-acquisition.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-image-acquisition.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-image-acquisition.png"})
2
La surface de l'échantillon nouvellement exposée de la structure d'intérêt est imagée. Ce processus de broyage et d'imagerie est répété jusqu'à ce que la structure soit complètement imagée.
![Traitement de la segmentation Traitement de la segmentation]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-processing-segmentation.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-processing-segmentation.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-processing-segmentation.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-processing-segmentation.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png"})
3
Les images acquises au microscope électronique sont traitées et alignées numériquement dans un ensemble de données 3D. Les compartiments cellulaires peuvent être identifiés et segmentés.
![Analyse de la visualisation en 3D Analyse de la visualisation en 3D]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-3d-visualization-analysis.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-3d-visualization-analysis.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-3d-visualization-analysis.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-3d-visualization-analysis.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png"})
4
L'ensemble des données 3D segmentées peut être visualisé, étudié et analysé statistiquement.
Exemples d'application
Visualisation isotrope à haute résolution de l'ultrastructure cellulaire en 3D
Imagerie 3D des cellules HeLa
Imagerie de série 3D automatisée avec la technologie FIB-SEM de ZEISS
Le faisceau d'ions focalisé a été utilisé pour enlever séquentiellement des couches de 8 nm d'épaisseur de l'échantillon pendant que la block-face exposée est scannée avec un microscope électronique à balayage. Un volume d'images 3D de haute résolution est ainsi obtenu. La segmentation et la visualisation automatisées des composants cellulaires ont été effectuées à l'aide d'un modèle d'apprentissage profond entraîné par arivis Cloud dans arivis Pro, afin que les différents composants cellulaires puissent être visualisés et quantifiés.
![Reconstruction 3D du corps de Golgi d'une algue à partir de données brutes de broyage FIB Reconstruction 3D du corps de Golgi d'une algue à partir de données brutes de broyage FIB]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.100.75.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.360.270.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.768.576.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.1024.768.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.1280.960.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.1280.960.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.1280.960.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg"})
Image reproduite avec l'aimable autorisation du Dr Louise Hughes, Université d'Oxford Brookes, Royaume-Uni.
Caractérisation de l'appareil de Golgi
Mieux comprendre son rôle dans la modification et le transport des protéines
Cette image montre une reconstruction 3D du corps de Golgi d'une algue à partir d'un ensemble de données FIB-SEM. L'ensemble des données distingue les faces cis et trans du Golgi (jaune/rouge : cis-golgi, violet/bleu : trans-golgi). La segmentation des composants cellulaires à partir des ensembles de données à haute résolution acquis à l'aide de la technologie ZEISS Crossbeam FIB-SEM permet de caractériser et de quantifier avec précision les composants internes.