![クライオFIB-SEMを用いたボリュームEM]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem.jpg/_jcr_content/renditions/original.image_file.100.56.file/cryo-fib-sem.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem.jpg/_jcr_content/renditions/original.image_file.360.203.file/cryo-fib-sem.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem.jpg/_jcr_content/renditions/original.image_file.768.432.file/cryo-fib-sem.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem.jpg/_jcr_content/renditions/original.image_file.1024.576.file/cryo-fib-sem.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem.jpg/_jcr_content/renditions/original.image_file.1280.720.file/cryo-fib-sem.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem.jpg/_jcr_content/renditions/original.image_file.1440.810.file/cryo-fib-sem.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem.jpg/_jcr_content/renditions/original./cryo-fib-sem.jpg"} "クライオFIB-SEMを用いたボリュームEM")
Volume EM
クライオFIB-SEM
超高分解能で実際の状況に近い状態の細胞ダイナミクスのスナップショットを取得
ワークフローの略図
![クライオFIB-SEMミリング]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-milling.png/_jcr_content/renditions/original.image_file.100.100.0,1,1280,1281.file/cryo-fib-sem-milling.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-milling.png/_jcr_content/renditions/original.image_file.360.360.0,1,1280,1281.file/cryo-fib-sem-milling.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-milling.png/_jcr_content/renditions/original.image_file.768.768.0,1,1280,1281.file/cryo-fib-sem-milling.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-milling.png/_jcr_content/renditions/original.image_file.1024.1024.0,1,1280,1281.file/cryo-fib-sem-milling.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/cryo-fib-sem-milling.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/cryo-fib-sem-milling.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/cryo-fib-sem-milling.png"} "クライオFIB-SEMミリング")
1
対象の構造が見えるようになるまで、未染色のガラス化試料に集束イオンビームで溝をミリングします。
![クライオFIB-SEMによる画像取得]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.100.100.0,1,1280,1281.file/cryo-fib-sem-image-acquisition.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.360.360.0,1,1280,1281.file/cryo-fib-sem-image-acquisition.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.768.768.0,1,1280,1281.file/cryo-fib-sem-image-acquisition.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1024.1024.0,1,1280,1281.file/cryo-fib-sem-image-acquisition.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/cryo-fib-sem-image-acquisition.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/cryo-fib-sem-image-acquisition.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/cryo-fib-sem-image-acquisition.png"} "クライオFIB-SEMによる画像取得")
2
対象の構造の新たに露出した試料表面をイメージングします。このミリングとイメージングのプロセスを、構造が完全にイメージングできるまで繰り返し行います。試料は、プロセス全体を通してガラス化した状態を維持します。
![セグメンテーションの処理]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-processing-segmentation.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-processing-segmentation.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-processing-segmentation.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-processing-segmentation.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png"} "セグメンテーションの処理")
3
取得した電子顕微鏡画像が処理され、デジタルに調整されて3Dデータセットになります。細胞コンパートメントを特定し、セグメント化することができます。
![3Dビジュアライゼーション解析]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-3d-visualization-analysis.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-3d-visualization-analysis.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-3d-visualization-analysis.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-3d-visualization-analysis.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png"} "3Dビジュアライゼーション解析")
4
セグメント化された3Dデータセットは、視覚化および調査を行い、統計的に分析することができます。
アプリケーション例
円石藻における方解石結晶のバイオミネラリゼーションプロセスを理解する
試料ご提供：S. Sviben & A. Scheffel, Max Planck Insititute Plant Physiology, and L. Bertinetti, Max-Planck Institute of Colloids and Interfaces, Potsdam-Golm, Germany
円石藻エミリアニア・ハクスレイにおける円石の形成
方解石粒とその微細構造環境のビジュアライゼーション
従来の水ベースの試料作製プロトコルでは、水溶性非結晶フェーズのカルシウムである円石藻の微細構造環境のイメージングは困難です。FIB-SEMを極低温条件下で操作することで、実際の状況に近い状態でガラス化した海藻類のイメージングが可能となり、微細構造を3Dで明瞭に観察できます。
ここでは、E. huxleyi（円石藻）の細胞を高圧凍結しました。イメージングプロセス中、試料は凍結状態で維持され、ZEISS Crossbeamを使用して極低温条件下でFIB-SEMデータを取得しました。3D再構築像は、成熟した円石（黄）、発生時の状態（青）と脂肪体（赤）の円石を示しています。
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/application-examples/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg/_jcr_content/renditions/original.image_file.100.56.0,50,1600,950.file/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/application-examples/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg/_jcr_content/renditions/original.image_file.360.203.0,50,1600,950.file/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/application-examples/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg/_jcr_content/renditions/original.image_file.768.432.0,50,1600,950.file/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/application-examples/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg/_jcr_content/renditions/original.image_file.1024.576.0,50,1600,950.file/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/application-examples/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg/_jcr_content/renditions/original.image_file.1280.720.0,50,1600,950.file/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/application-examples/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg/_jcr_content/renditions/original.image_file.1440.810.0,50,1600,950.file/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/application-examples/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg/_jcr_content/renditions/original.image_file.1600.900.0,50,1600,950.file/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg"})
![ZEISS Correlative Cryo Workflow]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.100.100.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.360.360.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.768.768.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.1024.1024.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.1280.1280.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.1280.1280.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.1280.1280.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg"} "ZEISS Correlative Cryo Workflow")
![ZEISS Crossbeam - 3D分析と試料作製を高いスループットで実現するFIB-SEM]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.100.100.file/crossbeam-550-system.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.360.360.file/crossbeam-550-system.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.768.768.file/crossbeam-550-system.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/crossbeam-550-system.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/crossbeam-550-system.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/crossbeam-550-system.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original./crossbeam-550-system.jpg"} "ZEISS Crossbeam")
![ZEISS arivis Cloudソフトウェアの画面を表示しているデスクトップモニター。内皮細胞のセグメンテーション予測が表示されている。各細胞は別々のオブジェクトとして検出されている。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original.image_file.100.100.file/zeiss-arivis-cloud-product-0724.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original.image_file.360.360.file/zeiss-arivis-cloud-product-0724.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original.image_file.768.768.file/zeiss-arivis-cloud-product-0724.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss-arivis-cloud-product-0724.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original./zeiss-arivis-cloud-product-0724.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original./zeiss-arivis-cloud-product-0724.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original./zeiss-arivis-cloud-product-0724.jpg"} "ZEISS arivis Cloudソフトウェア")
![FIB-SEM体積電子顕微鏡データの核膜孔複合体の解析結果を示すZEISS arivis Proソフトウェアの画面が表示されたデスクトップモニター。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.100.100.file/zeiss-arivis-pro-product-0724.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.360.360.file/zeiss-arivis-pro-product-0724.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.768.768.file/zeiss-arivis-pro-product-0724.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss-arivis-pro-product-0724.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original./zeiss-arivis-pro-product-0724.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original./zeiss-arivis-pro-product-0724.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original./zeiss-arivis-pro-product-0724.png"} "ZEISS arivis Proソフトウェア")