![集束イオンビーム走査型電子顕微鏡法を用いたVolume EM]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.100.56.file/fib-sem.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.360.203.file/fib-sem.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.768.432.file/fib-sem.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.1024.576.file/fib-sem.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.1280.720.file/fib-sem.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.1440.810.file/fib-sem.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original./fib-sem.jpg"} "集束イオンビーム走査型電子顕微鏡法を用いたVolume EM")
Volume EM
集束イオンビーム走査電子顕微鏡
正確な3D再構成を可能にする高分解能、アイソトロピックボリュームデータ
ワークフローの略図
![FIB-SEMミリング]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.100.100.0,1,1280,1281.file/fib-sem-milling.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.360.360.0,1,1280,1281.file/fib-sem-milling.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.768.768.0,1,1280,1281.file/fib-sem-milling.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.1024.1024.0,1,1280,1281.file/fib-sem-milling.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-milling.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-milling.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-milling.png"} "FIB-SEMミリング")
1
対象の構造が見えるまで、樹脂包埋した試料に集束イオンビームで溝をミリングします。
![FIN-SEMによる画像取得]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.100.100.0,1,1280,1281.file/fib-sem-image-acquisition.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.360.360.0,1,1280,1281.file/fib-sem-image-acquisition.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.768.768.0,1,1280,1281.file/fib-sem-image-acquisition.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1024.1024.0,1,1280,1281.file/fib-sem-image-acquisition.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-image-acquisition.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-image-acquisition.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-image-acquisition.png"} "FIN-SEMによる画像取得")
2
対象の構造の新たに露出した試料表面をイメージングします。このミリングとイメージングのプロセスを、構造が完全にイメージングできるまで繰り返し行います。
![セグメンテーションの処理]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-processing-segmentation.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-processing-segmentation.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-processing-segmentation.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-processing-segmentation.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png"} "セグメンテーションの処理")
3
取得した電子顕微鏡画像が処理され、デジタルに調整されて3Dデータセットになります。細胞コンパートメントを特定し、セグメント化することができます。
![3Dビジュアライゼーション解析]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-3d-visualization-analysis.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-3d-visualization-analysis.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-3d-visualization-analysis.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-3d-visualization-analysis.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png"} "3Dビジュアライゼーション解析")
4
セグメント化された3Dデータセットは、視覚化および調査を行い、統計的に分析することができます。
アプリケーション例
細胞微細構造の高分解能、アイソトロピック3Dビジュアライゼーション
データご提供：Anna Steyer and Yannick Schwab, EMBL Heidelberg, Germany
HeLa細胞の3Dイメージング
ZEISS FIB-SEMテクノロジーを用いた自動3D連続イメージング
集束イオンビームを使用して、試料から8 nm厚の層を連続で除去し、走査電子顕微鏡で露出したブロックフェースをスキャンすることにより、高分解能3Dボリューム画像を取得します。arivis Pro内のarivis Cloudでトレーニングした深層学習モデルを用いて細胞内容物の自動セグメンテーション、自動ビジュアライゼーションを行うことで、異なる細胞内容物を視覚化・定量化できます。
![FIBミリングのローデータに基づいた、藻類におけるゴルジ体の3D再構成]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.100.75.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.360.270.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.768.576.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.1024.768.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.1280.960.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.1280.960.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.1280.960.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg"} "FIBミリングのローデータに基づいた、藻類におけるゴルジ体の3D再構成")
画像ご提供：Dr Louise Hughes, Oxford Brookes University, UK
ゴルジ体の描出
タンパク質修飾と輸送におけるゴルジ体の役割を深く理解するために
この画像は、FIB-SEMデータセットからの藻類におけるゴルジ体の3D再構成を示しています。データセットが、ゴルジ体のシス面とトランス面を識別します（黄/赤：シスゴルジ、紫/青：トランスゴルジ）。ZEISS Crossbeam FIB-SEMテクノロジーを用いて取得した高分解能データを使用して細胞内容物をセグメンテーションすることにより、内容物を正確に描出するとともに定量化できるようになります。
![ZEISS Crossbeam - 3D分析と試料作製を高いスループットで実現するFIB-SEM]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.100.100.file/crossbeam-550-system.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.360.360.file/crossbeam-550-system.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.768.768.file/crossbeam-550-system.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/crossbeam-550-system.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/crossbeam-550-system.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/crossbeam-550-system.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original./crossbeam-550-system.jpg"} "ZEISS Crossbeam")
![ZEISS arivis Cloudソフトウェアの画面を表示しているデスクトップモニター。内皮細胞のセグメンテーション予測が表示されている。各細胞は別々のオブジェクトとして検出されている。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original.image_file.100.100.file/zeiss-arivis-cloud-product-0724.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original.image_file.360.360.file/zeiss-arivis-cloud-product-0724.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original.image_file.768.768.file/zeiss-arivis-cloud-product-0724.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss-arivis-cloud-product-0724.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original./zeiss-arivis-cloud-product-0724.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original./zeiss-arivis-cloud-product-0724.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original./zeiss-arivis-cloud-product-0724.jpg"} "ZEISS arivis Cloudソフトウェア")
![FIB-SEM体積電子顕微鏡データの核膜孔複合体の解析結果を示すZEISS arivis Proソフトウェアの画面が表示されたデスクトップモニター。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.100.100.file/zeiss-arivis-pro-product-0724.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.360.360.file/zeiss-arivis-pro-product-0724.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.768.768.file/zeiss-arivis-pro-product-0724.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss-arivis-pro-product-0724.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original./zeiss-arivis-pro-product-0724.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original./zeiss-arivis-pro-product-0724.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original./zeiss-arivis-pro-product-0724.png"} "ZEISS arivis Proソフトウェア")