![ZEISS Apotome 3]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-3-stage.jpg/_jcr_content/renditions/original.image_file.100.100.240,0,1680,1440.file/zeiss_apotome-3-stage.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-3-stage.jpg/_jcr_content/renditions/original.image_file.360.360.240,0,1680,1440.file/zeiss_apotome-3-stage.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-3-stage.jpg/_jcr_content/renditions/original.image_file.768.768.240,0,1680,1440.file/zeiss_apotome-3-stage.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-3-stage.jpg/_jcr_content/renditions/original.image_file.1024.1024.240,0,1680,1440.file/zeiss_apotome-3-stage.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-3-stage.jpg/_jcr_content/renditions/original.image_file.1280.1280.240,0,1680,1440.file/zeiss_apotome-3-stage.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-3-stage.jpg/_jcr_content/renditions/original.image_file.1440.1440.240,0,1680,1440.file/zeiss_apotome-3-stage.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-3-stage.jpg/_jcr_content/renditions/original.image_file.1440.1440.240,0,1680,1440.file/zeiss_apotome-3-stage.jpg"} "ZEISS Apotome 3")
ZEISS Apotome 3 ワイドフィールド顕微鏡を使った蛍光イメージングにおける光学セクショニング
構造化照明を用いることで、焦点面外で発生した光を簡単に効率よく排除できます。ZEISS Apotome 3は、異なるグリッド位置で取得した複数の画像から光学断面を再構築します。厚みのある試料であっても、簡単な操作でコントラストの高い画像が取得できます。
![トランスジェニックゼブラフィッシュの幼生。ご提供：H. Reuter, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit1.jpg/_jcr_content/renditions/original.image_file.100.50.6,0,1920,957.file/zeiss_apotome-benefit1.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit1.jpg/_jcr_content/renditions/original.image_file.360.180.6,0,1920,957.file/zeiss_apotome-benefit1.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit1.jpg/_jcr_content/renditions/original.image_file.768.384.6,0,1920,957.file/zeiss_apotome-benefit1.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit1.jpg/_jcr_content/renditions/original.image_file.1024.512.6,0,1920,957.file/zeiss_apotome-benefit1.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit1.jpg/_jcr_content/renditions/original.image_file.1280.640.6,0,1920,957.file/zeiss_apotome-benefit1.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit1.jpg/_jcr_content/renditions/original.image_file.1440.720.6,0,1920,957.file/zeiss_apotome-benefit1.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit1.jpg/_jcr_content/renditions/original.image_file.1914.957.6,0,1920,957.file/zeiss_apotome-benefit1.jpg"} "トランスジェニックゼブラフィッシュの幼生。ご提供：H. Reuter, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany")
鮮明な光学断面
厚みのある試料にも対応
Apotome 3では、従来の蛍光顕微鏡と比較して、Z軸方向の分解能が大幅に向上します。そのため、厚みのある試料からも3D再構築が可能な光学断面を得ることができます。異なるサイズの3種類のグリッドによって、どの対物レンズを使った場合でも最適な分解能が得られます。最適なグリッドが自動的に選択され、常にコントラストの高い光学断面が得られるため、実験に集中することができます。
キャプション：トランスジェニックゼブラフィッシュの幼生。ご提供：H. Reuter, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany
![皮質ニューロン（左：ワイドフィールド、右：Apotome 3）。ご提供：L. Behrendt, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit2-left.jpg/_jcr_content/renditions/original.image_file.100.100.file/zeiss_apotome-benefit2-left.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit2-left.jpg/_jcr_content/renditions/original.image_file.360.360.file/zeiss_apotome-benefit2-left.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit2-left.jpg/_jcr_content/renditions/original.image_file.768.768.file/zeiss_apotome-benefit2-left.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit2-left.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss_apotome-benefit2-left.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit2-left.jpg/_jcr_content/renditions/original./zeiss_apotome-benefit2-left.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit2-left.jpg/_jcr_content/renditions/original./zeiss_apotome-benefit2-left.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit2-left.jpg/_jcr_content/renditions/original./zeiss_apotome-benefit2-left.jpg"} "皮質ニューロン（ワイドフィールド）")
![皮質ニューロン（左：ワイドフィールド、右：Apotome 3）。ご提供：L. Behrendt, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit2-right.jpg/_jcr_content/renditions/original.image_file.100.100.file/zeiss_apotome-benefit2-right.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit2-right.jpg/_jcr_content/renditions/original.image_file.360.360.file/zeiss_apotome-benefit2-right.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit2-right.jpg/_jcr_content/renditions/original.image_file.768.768.file/zeiss_apotome-benefit2-right.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit2-right.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss_apotome-benefit2-right.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit2-right.jpg/_jcr_content/renditions/original./zeiss_apotome-benefit2-right.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit2-right.jpg/_jcr_content/renditions/original./zeiss_apotome-benefit2-right.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit2-right.jpg/_jcr_content/renditions/original./zeiss_apotome-benefit2-right.jpg"} "皮質ニューロン（Apotome 3）")
学術的に評価されたアルゴリズム
プロセスの見える正確な光学セクショニング
昨今のソフトウェアベースのソリューションは、試料に関する予備知識（AIベースの手法）を必要とするか、あるいは検証されていない複雑なアルゴリズムに依存しています。ユーザーはそういった仕組みの見えない解析で得られた画像を信頼するほかありません。一方、ZEISS Apotome 3はプロセスの見える学術的に評価されたアルゴリズムを採用しており、正確で信頼できる光学断面を取得できます。
キャプション：皮質ニューロン（左：ワイドフィールド、右：Apotome 3）。ご提供：L. Behrendt, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany
![皮質ニューロン。画像1 - ワイドフィールド、画像2 - Apotome 3、画像3 - Apotome 3 + デコンボリューション]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/widefield-apotome-dcv-comparison.jpg/_jcr_content/renditions/original.image_file.100.100.file/widefield-apotome-dcv-comparison.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/widefield-apotome-dcv-comparison.jpg/_jcr_content/renditions/original.image_file.360.360.file/widefield-apotome-dcv-comparison.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/widefield-apotome-dcv-comparison.jpg/_jcr_content/renditions/original.image_file.768.768.file/widefield-apotome-dcv-comparison.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/widefield-apotome-dcv-comparison.jpg/_jcr_content/renditions/original./widefield-apotome-dcv-comparison.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/widefield-apotome-dcv-comparison.jpg/_jcr_content/renditions/original./widefield-apotome-dcv-comparison.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/widefield-apotome-dcv-comparison.jpg/_jcr_content/renditions/original./widefield-apotome-dcv-comparison.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/widefield-apotome-dcv-comparison.jpg/_jcr_content/renditions/original./widefield-apotome-dcv-comparison.jpg"} "皮質ニューロン")
皮質ニューロン
詳細な構造情報
ワイドフィールド、光学セクショニング、デコンボリューションによる画像の比較
構造化照明用のアルゴリズムを使用したデコンボリューションにより、得られた画像をさらに鮮明にすることができます。すべての生データを保持しながら、ワイドフィールド、光学断面、デコンボリューションによる画像を自由に切り替えて比較可能です。信頼できる、使いやすいデコンボリューションアルゴリズムにより、水平方向とZ軸方向の分解能が向上します。コントラストとノイズ抑制の向上により、試料の構造が明瞭になります。
キャプション：皮質ニューロン。ご提供：L. Behrendt, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany
![自由に選べる光源と蛍光色素]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit4.jpg/_jcr_content/renditions/original.image_file.100.50.file/zeiss_apotome-benefit4.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit4.jpg/_jcr_content/renditions/original.image_file.360.180.file/zeiss_apotome-benefit4.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit4.jpg/_jcr_content/renditions/original.image_file.768.384.file/zeiss_apotome-benefit4.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit4.jpg/_jcr_content/renditions/original.image_file.1024.512.file/zeiss_apotome-benefit4.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit4.jpg/_jcr_content/renditions/original.image_file.1280.640.file/zeiss_apotome-benefit4.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit4.jpg/_jcr_content/renditions/original.image_file.1440.720.file/zeiss_apotome-benefit4.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/zeiss_apotome-benefit4.jpg/_jcr_content/renditions/original./zeiss_apotome-benefit4.jpg"} "自由に選べる光源と蛍光色素")
自由に選べる光源と蛍光色素
決めるのはテクノロジーではなく、ユーザーです
実験の複雑さや要件は、時とともに変化するものです。だからこそ、変化に柔軟に対応できる装置が必要です。Apotome 3では、メタルハライドランプ、経済的な白色LED、試料ダメージを抑えたマルチカラーのLED光源Colibriをご使用いただけます。DAPI、Alexa488、Rhodamin、Cy5、またはGFPやmCherryなどの主要な色素を使用する場合でも、Apotome 3は蛍光色素と光源に適合し、期待通りのシャープで鮮やかな画像を作成します。
![顕微鏡]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_microscope-02.jpg/_jcr_content/renditions/original.image_file.100.56.file/apotome_components_microscope-02.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_microscope-02.jpg/_jcr_content/renditions/original.image_file.360.203.file/apotome_components_microscope-02.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_microscope-02.jpg/_jcr_content/renditions/original.image_file.768.432.file/apotome_components_microscope-02.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_microscope-02.jpg/_jcr_content/renditions/original.image_file.1024.576.file/apotome_components_microscope-02.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_microscope-02.jpg/_jcr_content/renditions/original.image_file.1280.720.file/apotome_components_microscope-02.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_microscope-02.jpg/_jcr_content/renditions/original.image_file.1440.810.file/apotome_components_microscope-02.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_microscope-02.jpg/_jcr_content/renditions/original./apotome_components_microscope-02.jpg"} "顕微鏡")
![推奨対物レンズ]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_objectives.jpg/_jcr_content/renditions/original.image_file.100.56.0,100,1920,1180.file/apotome_components_objectives.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_objectives.jpg/_jcr_content/renditions/original.image_file.360.203.0,100,1920,1180.file/apotome_components_objectives.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_objectives.jpg/_jcr_content/renditions/original.image_file.768.432.0,100,1920,1180.file/apotome_components_objectives.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_objectives.jpg/_jcr_content/renditions/original.image_file.1024.576.0,100,1920,1180.file/apotome_components_objectives.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_objectives.jpg/_jcr_content/renditions/original.image_file.1280.720.0,100,1920,1180.file/apotome_components_objectives.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_objectives.jpg/_jcr_content/renditions/original.image_file.1440.810.0,100,1920,1180.file/apotome_components_objectives.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_objectives.jpg/_jcr_content/renditions/original.image_file.1920.1080.0,100,1920,1180.file/apotome_components_objectives.jpg"} "推奨対物レンズ")
![光源Colibri 7]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_illumination-02.jpg/_jcr_content/renditions/original.image_file.100.56.file/apotome_components_illumination-02.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_illumination-02.jpg/_jcr_content/renditions/original.image_file.360.203.file/apotome_components_illumination-02.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_illumination-02.jpg/_jcr_content/renditions/original.image_file.768.432.file/apotome_components_illumination-02.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_illumination-02.jpg/_jcr_content/renditions/original.image_file.1024.576.file/apotome_components_illumination-02.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_illumination-02.jpg/_jcr_content/renditions/original.image_file.1280.720.file/apotome_components_illumination-02.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_illumination-02.jpg/_jcr_content/renditions/original.image_file.1440.810.file/apotome_components_illumination-02.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_illumination-02.jpg/_jcr_content/renditions/original./apotome_components_illumination-02.jpg"} "照明")
![カメラAxiocam 712 mono]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_cameras-01.jpg/_jcr_content/renditions/original.image_file.100.56.file/apotome_components_cameras-01.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_cameras-01.jpg/_jcr_content/renditions/original.image_file.360.203.file/apotome_components_cameras-01.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_cameras-01.jpg/_jcr_content/renditions/original.image_file.768.432.file/apotome_components_cameras-01.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_cameras-01.jpg/_jcr_content/renditions/original.image_file.1024.576.file/apotome_components_cameras-01.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_cameras-01.jpg/_jcr_content/renditions/original.image_file.1280.720.file/apotome_components_cameras-01.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_cameras-01.jpg/_jcr_content/renditions/original.image_file.1440.810.file/apotome_components_cameras-01.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome_components_cameras-01.jpg/_jcr_content/renditions/original./apotome_components_cameras-01.jpg"} "カメラ")
![バックグラウンドテクノロジー：構造化照明を使用した光学セクショニングにより、焦点面外で発生する光を効率的に抑え、鮮明な画像や3Dレンダリングの作成が可能です。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome-3_beam-path.jpg/_jcr_content/renditions/original.image_file.100.75.0,288,1917,1727.file/apotome-3_beam-path.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome-3_beam-path.jpg/_jcr_content/renditions/original.image_file.360.270.0,288,1917,1727.file/apotome-3_beam-path.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome-3_beam-path.jpg/_jcr_content/renditions/original.image_file.768.576.0,288,1917,1727.file/apotome-3_beam-path.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome-3_beam-path.jpg/_jcr_content/renditions/original.image_file.1024.768.0,288,1917,1727.file/apotome-3_beam-path.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome-3_beam-path.jpg/_jcr_content/renditions/original.image_file.1280.960.0,288,1917,1727.file/apotome-3_beam-path.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome-3_beam-path.jpg/_jcr_content/renditions/original.image_file.1440.1080.0,288,1917,1727.file/apotome-3_beam-path.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome-3_beam-path.jpg/_jcr_content/renditions/original.image_file.1917.1438.0,288,1917,1727.file/apotome-3_beam-path.jpg"} "バックグラウンドテクノロジー")
![バックグラウンドテクノロジー構造化照明を使用した光学セクショニングにより、焦点面外で発生する光を効率的に抑え、鮮明な画像や3Dレンダリングの作成が可能です。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome-3_beam-path.jpg/_jcr_content/renditions/original.image_file.100.100.file/apotome-3_beam-path.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome-3_beam-path.jpg/_jcr_content/renditions/original.image_file.360.360.file/apotome-3_beam-path.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome-3_beam-path.jpg/_jcr_content/renditions/original.image_file.768.768.file/apotome-3_beam-path.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome-3_beam-path.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/apotome-3_beam-path.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome-3_beam-path.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/apotome-3_beam-path.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome-3_beam-path.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/apotome-3_beam-path.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome-3_beam-path.jpg/_jcr_content/renditions/original./apotome-3_beam-path.jpg"} "バックグラウンドテクノロジー")
![A：ワイドフィールド画像。B～D：異なるグリッド位置でのオリジナル画像。E：最終画像、構造化照明により焦点面外で発生した光が排除されています。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grid-sections.jpg/_jcr_content/renditions/original.image_file.100.56.0,0,1198,674.file/apotome-grid-sections.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grid-sections.jpg/_jcr_content/renditions/original.image_file.360.203.0,0,1198,674.file/apotome-grid-sections.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grid-sections.jpg/_jcr_content/renditions/original.image_file.768.432.0,0,1198,674.file/apotome-grid-sections.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grid-sections.jpg/_jcr_content/renditions/original.image_file.1024.576.0,0,1198,674.file/apotome-grid-sections.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grid-sections.jpg/_jcr_content/renditions/original.image_file.1198.674.0,0,1198,674.file/apotome-grid-sections.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grid-sections.jpg/_jcr_content/renditions/original.image_file.1198.674.0,0,1198,674.file/apotome-grid-sections.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grid-sections.jpg/_jcr_content/renditions/original.image_file.1198.674.0,0,1198,674.file/apotome-grid-sections.jpg"} "Apotome 3による画像構築の仕組み")
![A：ワイドフィールド画像。B～D：異なるグリッド位置でのオリジナル画像。E：最終画像、構造化照明により焦点面外で発生した光が排除されています。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grid-sections.jpg/_jcr_content/renditions/original.image_file.100.56.file/apotome-grid-sections.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grid-sections.jpg/_jcr_content/renditions/original.image_file.360.203.file/apotome-grid-sections.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grid-sections.jpg/_jcr_content/renditions/original.image_file.768.432.file/apotome-grid-sections.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grid-sections.jpg/_jcr_content/renditions/original.image_file.1024.576.file/apotome-grid-sections.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grid-sections.jpg/_jcr_content/renditions/original./apotome-grid-sections.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grid-sections.jpg/_jcr_content/renditions/original./apotome-grid-sections.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grid-sections.jpg/_jcr_content/renditions/original./apotome-grid-sections.jpg"} "Apotome 3による画像構築の仕組み")
A：ワイドフィールド画像。B～D：異なるグリッド位置でのオリジナル画像。E：最終画像、構造化照明により焦点面外で発生した光が排除されています。
A：ワイドフィールド画像。B～D：異なるグリッド位置でのオリジナル画像。E：最終画像、構造化照明により焦点面外で発生した光が排除されています。
Apotome 3による画像構築の仕組み
Apotome 3は、グリッドを使用して輝度差のパターンを生成します。試料のある領域に焦点面外の光が存在する場合、そこではグリッドパターンも見えなくなります。グリッド位置の蛍光を取得後、グリッドは次の位置に移動します。これにより、高コントラスト・高分解能の、真の光学断面が導き出されます。
![C. elegans、ホールマウント、緑：GFP、青：DAPI。対物レンズ：Plan-Apochromat 20x/0.8。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grids.jpg/_jcr_content/renditions/original.image_file.100.56.0,0,1198,674.file/apotome-grids.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grids.jpg/_jcr_content/renditions/original.image_file.360.203.0,0,1198,674.file/apotome-grids.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grids.jpg/_jcr_content/renditions/original.image_file.768.432.0,0,1198,674.file/apotome-grids.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grids.jpg/_jcr_content/renditions/original.image_file.1024.576.0,0,1198,674.file/apotome-grids.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grids.jpg/_jcr_content/renditions/original.image_file.1198.674.0,0,1198,674.file/apotome-grids.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grids.jpg/_jcr_content/renditions/original.image_file.1198.674.0,0,1198,674.file/apotome-grids.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grids.jpg/_jcr_content/renditions/original.image_file.1198.674.0,0,1198,674.file/apotome-grids.jpg"} "C. elegans、ホールマウント")
![C. elegans、ホールマウント、緑：GFP、青：DAPI。対物レンズ：Plan-Apochromat 20x/0.8。 ご提供：Prof. Schnabel, T.U. Braunschweig, Germany]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grids.jpg/_jcr_content/renditions/original.image_file.100.56.file/apotome-grids.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grids.jpg/_jcr_content/renditions/original.image_file.360.203.file/apotome-grids.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grids.jpg/_jcr_content/renditions/original.image_file.768.432.file/apotome-grids.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grids.jpg/_jcr_content/renditions/original.image_file.1024.576.file/apotome-grids.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grids.jpg/_jcr_content/renditions/original./apotome-grids.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grids.jpg/_jcr_content/renditions/original./apotome-grids.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/apotome-grids.jpg/_jcr_content/renditions/original./apotome-grids.jpg"} "C. elegans、ホールマウント ご提供：Prof. Schnabel, T.U. Braunschweig, Germany")
C. elegans、ホールマウント、緑：GFP、青：DAPI。対物レンズ：Plan-Apochromat 20x/0.8。
C. elegans、ホールマウント、緑：GFP、青：DAPI。対物レンズ：Plan-Apochromat 20x/0.8。ご提供：Prof. Schnabel, T.U. Braunschweig, Germany
試料に最適な光学断面厚を選択
Apotome 3は、どの倍率を使用しても、ビームパスに最適なグリッドを自動的に選択します。
A：焦点面外で発生した光が検出され、コントラストと分解能が低下しています。B：グリッド周波数の増加とともに、不要なバックグラウンド蛍光が低減し、光学断面は薄くなっていきます。C：焦点面の外側からの画像成分が抑制され、光学断面のコントラストおよび分解能が向上します。D：この例では、低倍率用グリッドを使用した場合に、最適な光学断面厚が得られました。この種の画像は、3D解析や画像再構築ソフトウェアを用いたデータ処理に最適です。
![DNAと微小管を染色した大脳皮質神経細胞の3D再構築。ご提供：L. Behrendt, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-right.jpg/_jcr_content/renditions/original.image_file.100.50.598,598,1601,1099.file/apotome_applications_drosophila-embrio-right.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-right.jpg/_jcr_content/renditions/original.image_file.360.180.598,598,1601,1099.file/apotome_applications_drosophila-embrio-right.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-right.jpg/_jcr_content/renditions/original.image_file.768.384.598,598,1601,1099.file/apotome_applications_drosophila-embrio-right.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-right.jpg/_jcr_content/renditions/original.image_file.1002.501.598,598,1601,1099.file/apotome_applications_drosophila-embrio-right.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-right.jpg/_jcr_content/renditions/original.image_file.1002.501.598,598,1601,1099.file/apotome_applications_drosophila-embrio-right.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-right.jpg/_jcr_content/renditions/original.image_file.1002.501.598,598,1601,1099.file/apotome_applications_drosophila-embrio-right.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-right.jpg/_jcr_content/renditions/original.image_file.1002.501.598,598,1601,1099.file/apotome_applications_drosophila-embrio-right.jpg"} "DNAと微小管を染色した大脳皮質神経細胞の3D再構築")
ZEISS Apotome 3のアプリケーション
アプリケーション例
![従来の蛍光イメージング - ショウジョウバエの神経細胞、青：DAPI、黄：GFP。対物レンズ：Plan-Apochromat 20x/0.8。ご提供：M. Koch, Molecular and Developmental Genetics, University of Leuven, Belgium]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-neurons.jpg/_jcr_content/renditions/original.image_file.100.75.0,0,959,722.file/apotome_applications_drosophila-neurons.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-neurons.jpg/_jcr_content/renditions/original.image_file.360.270.0,0,959,722.file/apotome_applications_drosophila-neurons.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-neurons.jpg/_jcr_content/renditions/original.image_file.768.576.0,0,959,722.file/apotome_applications_drosophila-neurons.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-neurons.jpg/_jcr_content/renditions/original.image_file.959.719.0,0,959,722.file/apotome_applications_drosophila-neurons.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-neurons.jpg/_jcr_content/renditions/original.image_file.959.719.0,0,959,722.file/apotome_applications_drosophila-neurons.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-neurons.jpg/_jcr_content/renditions/original.image_file.959.719.0,0,959,722.file/apotome_applications_drosophila-neurons.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-neurons.jpg/_jcr_content/renditions/original.image_file.959.719.0,0,959,722.file/apotome_applications_drosophila-neurons.jpg"} "従来の蛍光イメージング - ショウジョウバエの神経細胞")
![Apotome 3 - ショウジョウバエの神経細胞、青：DAPI、黄：GFP。対物レンズ：Plan-Apochromat 20x/0.8。ご提供：M. Koch, Molecular and Developmental Genetics, University of Leuven, Belgium]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-neurons.jpg/_jcr_content/renditions/original.image_file.100.75.962,0,1919,719.file/apotome_applications_drosophila-neurons.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-neurons.jpg/_jcr_content/renditions/original.image_file.360.270.962,0,1919,719.file/apotome_applications_drosophila-neurons.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-neurons.jpg/_jcr_content/renditions/original.image_file.768.576.962,0,1919,719.file/apotome_applications_drosophila-neurons.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-neurons.jpg/_jcr_content/renditions/original.image_file.957.718.962,0,1919,719.file/apotome_applications_drosophila-neurons.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-neurons.jpg/_jcr_content/renditions/original.image_file.957.718.962,0,1919,719.file/apotome_applications_drosophila-neurons.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-neurons.jpg/_jcr_content/renditions/original.image_file.957.718.962,0,1919,719.file/apotome_applications_drosophila-neurons.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-neurons.jpg/_jcr_content/renditions/original.image_file.957.718.962,0,1919,719.file/apotome_applications_drosophila-neurons.jpg"} "Apotome3 - ショウジョウバエの神経細胞")
ショウジョウバエの神経細胞
ショウジョウバエの神経細胞、青：DAPI、黄：GFP。対物レンズ：Plan-Apochromat 20x/0.8。ご提供：M. Koch, Molecular and Developmental Genetics, University of Leuven, Belgium
ショウジョウバエ胚
ショウジョウバエの胚、緑：HRP、赤：グリアマーカー、100 µm Zスタック。ご提供：C. Klämbt, Institute for Neurobiology, University of Münster, Germany
![DNAと微小管を染色した皮質ニューロンのワイドフィールド画像と3D再構築画像の比較。ご提供：L. Behrendt, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-left.jpg/_jcr_content/renditions/original.image_file.100.75.4,0,1596,1194.file/apotome_applications_drosophila-embrio-left.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-left.jpg/_jcr_content/renditions/original.image_file.360.270.4,0,1596,1194.file/apotome_applications_drosophila-embrio-left.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-left.jpg/_jcr_content/renditions/original.image_file.768.576.4,0,1596,1194.file/apotome_applications_drosophila-embrio-left.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-left.jpg/_jcr_content/renditions/original.image_file.1024.768.4,0,1596,1194.file/apotome_applications_drosophila-embrio-left.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-left.jpg/_jcr_content/renditions/original.image_file.1280.960.4,0,1596,1194.file/apotome_applications_drosophila-embrio-left.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-left.jpg/_jcr_content/renditions/original.image_file.1440.1080.4,0,1596,1194.file/apotome_applications_drosophila-embrio-left.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-left.jpg/_jcr_content/renditions/original.image_file.1592.1194.4,0,1596,1194.file/apotome_applications_drosophila-embrio-left.jpg"} "ワイドフィールド - DNAと微小管を染色した皮質ニューロン")
![DNAと微小管を染色した皮質ニューロンのワイドフィールド画像と3D再構築画像の比較。ご提供：L. Behrendt, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-right.jpg/_jcr_content/renditions/original.image_file.100.75.4,0,1596,1194.file/apotome_applications_drosophila-embrio-right.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-right.jpg/_jcr_content/renditions/original.image_file.360.270.4,0,1596,1194.file/apotome_applications_drosophila-embrio-right.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-right.jpg/_jcr_content/renditions/original.image_file.768.576.4,0,1596,1194.file/apotome_applications_drosophila-embrio-right.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-right.jpg/_jcr_content/renditions/original.image_file.1024.768.4,0,1596,1194.file/apotome_applications_drosophila-embrio-right.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-right.jpg/_jcr_content/renditions/original.image_file.1280.960.4,0,1596,1194.file/apotome_applications_drosophila-embrio-right.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-right.jpg/_jcr_content/renditions/original.image_file.1440.1080.4,0,1596,1194.file/apotome_applications_drosophila-embrio-right.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/apotome_applications_drosophila-embrio-right.jpg/_jcr_content/renditions/original.image_file.1592.1194.4,0,1596,1194.file/apotome_applications_drosophila-embrio-right.jpg"} "Apotome 3 - DNAと微小管を染色した大脳皮質神経細胞の3D再構築")
皮質ニューロン
DNAと微小管を染色した皮質ニューロンのワイドフィールド画像と3D再構築画像の比較。ご提供：L. Behrendt, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany
![左から順に：ワイドフィールド、Apotome 3、Apotome 3 + デコンボリューション]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/lotus-japonicus-root-apotome-3.jpg/_jcr_content/renditions/original.image_file.100.75.0,0,1917,1439.file/lotus-japonicus-root-apotome-3.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/lotus-japonicus-root-apotome-3.jpg/_jcr_content/renditions/original.image_file.360.270.0,0,1917,1439.file/lotus-japonicus-root-apotome-3.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/lotus-japonicus-root-apotome-3.jpg/_jcr_content/renditions/original.image_file.768.576.0,0,1917,1439.file/lotus-japonicus-root-apotome-3.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/lotus-japonicus-root-apotome-3.jpg/_jcr_content/renditions/original.image_file.1024.768.0,0,1917,1439.file/lotus-japonicus-root-apotome-3.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/lotus-japonicus-root-apotome-3.jpg/_jcr_content/renditions/original.image_file.1280.960.0,0,1917,1439.file/lotus-japonicus-root-apotome-3.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/lotus-japonicus-root-apotome-3.jpg/_jcr_content/renditions/original.image_file.1440.1080.0,0,1917,1439.file/lotus-japonicus-root-apotome-3.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/lotus-japonicus-root-apotome-3.jpg/_jcr_content/renditions/original.image_file.1917.1438.0,0,1917,1439.file/lotus-japonicus-root-apotome-3.jpg"} "ミヤコグサ（Lotus Japonicus）の根")
ミヤコグサ（Lotus Japonicus）の根
![左から順に：ワイドフィールド、Apotome 3、Apotome 3 + デコンボリューション]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/lotus-japonicus-root-apotome-3.jpg/_jcr_content/renditions/original.image_file.100.75.file/lotus-japonicus-root-apotome-3.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/lotus-japonicus-root-apotome-3.jpg/_jcr_content/renditions/original.image_file.360.270.file/lotus-japonicus-root-apotome-3.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/lotus-japonicus-root-apotome-3.jpg/_jcr_content/renditions/original.image_file.768.576.file/lotus-japonicus-root-apotome-3.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/lotus-japonicus-root-apotome-3.jpg/_jcr_content/renditions/original.image_file.1024.768.file/lotus-japonicus-root-apotome-3.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/lotus-japonicus-root-apotome-3.jpg/_jcr_content/renditions/original.image_file.1280.960.file/lotus-japonicus-root-apotome-3.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/lotus-japonicus-root-apotome-3.jpg/_jcr_content/renditions/original.image_file.1440.1080.file/lotus-japonicus-root-apotome-3.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/lotus-japonicus-root-apotome-3.jpg/_jcr_content/renditions/original./lotus-japonicus-root-apotome-3.jpg"} "ミヤコグサ（Lotus Japonicus）の根")
左から順に：ワイドフィールド、Apotome 3、Apotome 3 + デコンボリューション
左から順に：ワイドフィールド、Apotome 3、Apotome 3 + デコンボリューション
ミヤコグサ（Lotus Japonicus）の根
共生細菌（mCherry染色）が感染したミヤコグサ（Lotus Japonicus）の根の自家蛍光。ご提供：F. A. Ditengou, University of Freiburg, Germany
![上から順に：ワイドフィールド、Apotome 3、Apotome 3 + デコンボリューション]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/zebrafish-apotome-3.jpg/_jcr_content/renditions/original.image_file.100.75.0,0,1917,1439.file/zebrafish-apotome-3.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/zebrafish-apotome-3.jpg/_jcr_content/renditions/original.image_file.360.270.0,0,1917,1439.file/zebrafish-apotome-3.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/zebrafish-apotome-3.jpg/_jcr_content/renditions/original.image_file.768.576.0,0,1917,1439.file/zebrafish-apotome-3.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/zebrafish-apotome-3.jpg/_jcr_content/renditions/original.image_file.1024.768.0,0,1917,1439.file/zebrafish-apotome-3.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/zebrafish-apotome-3.jpg/_jcr_content/renditions/original.image_file.1280.960.0,0,1917,1439.file/zebrafish-apotome-3.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/zebrafish-apotome-3.jpg/_jcr_content/renditions/original.image_file.1440.1080.0,0,1917,1439.file/zebrafish-apotome-3.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/zebrafish-apotome-3.jpg/_jcr_content/renditions/original.image_file.1917.1438.0,0,1917,1439.file/zebrafish-apotome-3.jpg"} "トランスジェニックゼブラフィッシュの幼生")
トランスジェニックゼブラフィッシュの幼生
![上から順に：ワイドフィールド、Apotome 3、Apotome 3 + デコンボリューション]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/zebrafish-apotome-3.jpg/_jcr_content/renditions/original.image_file.100.75.file/zebrafish-apotome-3.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/zebrafish-apotome-3.jpg/_jcr_content/renditions/original.image_file.360.270.file/zebrafish-apotome-3.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/zebrafish-apotome-3.jpg/_jcr_content/renditions/original.image_file.768.576.file/zebrafish-apotome-3.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/zebrafish-apotome-3.jpg/_jcr_content/renditions/original.image_file.1024.768.file/zebrafish-apotome-3.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/zebrafish-apotome-3.jpg/_jcr_content/renditions/original.image_file.1280.960.file/zebrafish-apotome-3.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/zebrafish-apotome-3.jpg/_jcr_content/renditions/original.image_file.1440.1080.file/zebrafish-apotome-3.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/apotome-3/applications/zebrafish-apotome-3.jpg/_jcr_content/renditions/original./zebrafish-apotome-3.jpg"} "トランスジェニックゼブラフィッシュの幼生")
上から順に：ワイドフィールド、Apotome 3、Apotome 3 + デコンボリューション
上から順に：ワイドフィールド、Apotome 3、Apotome 3 + デコンボリューション
トランスジェニックゼブラフィッシュの幼生
受精後4日目のトランスジェニックゼブラフィッシュの幼生を染色：グリア線維性酸性タンパク質、アセチル化チューブリン、GFP、DNA。1.2%低融点アガロースに包埋。ご提供：H. Reuter, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany
![ZEISS Apotome 3のプレビュー画像]({"max":"https://asset-downloads.zeiss.com/catalogs/preview/mic/1a392174-f343-4c25-8a40-19203381665f/JP_product-info_Apotome3_rel2-0.pdf.jpg"} "ZEISS Apotome 3をダウンロード")
![構造化照明を使って信頼性 の高い光学断面を作成のプレビュー画像]({"max":"https://asset-downloads.zeiss.com/catalogs/preview/mic/b52bccf9-f8c1-4f3e-80ca-cae92b2b52b0/JP_product-flyer_Apotome-3_Creating-reliable-optical-sections.pdf.jpg"} "構造化照明を使って信頼性 の高い光学断面を作成をダウンロード")
![ZEISS Axio Imager 2]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-imager-2-for-bio-life-science/axio-imager-2_apotome-3_system.jpg/_jcr_content/renditions/original.image_file.100.100.file/axio-imager-2_apotome-3_system.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-imager-2-for-bio-life-science/axio-imager-2_apotome-3_system.jpg/_jcr_content/renditions/original.image_file.360.360.file/axio-imager-2_apotome-3_system.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-imager-2-for-bio-life-science/axio-imager-2_apotome-3_system.jpg/_jcr_content/renditions/original.image_file.768.768.file/axio-imager-2_apotome-3_system.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-imager-2-for-bio-life-science/axio-imager-2_apotome-3_system.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/axio-imager-2_apotome-3_system.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-imager-2-for-bio-life-science/axio-imager-2_apotome-3_system.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/axio-imager-2_apotome-3_system.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-imager-2-for-bio-life-science/axio-imager-2_apotome-3_system.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/axio-imager-2_apotome-3_system.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-imager-2-for-bio-life-science/axio-imager-2_apotome-3_system.jpg/_jcr_content/renditions/original./axio-imager-2_apotome-3_system.jpg"} "ZEISS Axio Imager 2")
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