Next generation of Lattice Light-Sheet Microscopy

28 April 2022

Press Contact for ZEISS Microscopy Vybhav Sinha

Jena, Germany | 28 April 2022 | ZEISS Research Microscopy Solutions

Today, ZEISS releases the next generation of ZEISS Lattice Lightsheet 7. The microscope system was introduced to the market in December 2020 and opened up a new way for researchers to explore the dynamics of life at subcellular resolution. It was the first commercial, easy-to-use implementation of the lattice light-sheet technology known for enabling sample-preserving, long-term imaging of living cells. The new generation of ZEISS Lattice Lightsheet 7 now provides a number of improvements to the life science research community that will substantially advance live cell experiments.

ZEISS Lattice Lightsheet 7 with Hamamatsu ORCA-Fusion camera.

Improved gentleness

Equipped with a more powerful camera, the Hamamatsu ORCA-Fusion, the microscope now enables imaging just above the noise level, making lattice light-sheet microscopy even gentler to light-sensitive samples. At the lowest photon count rates, the system provides both an unprecedented uniform background and a sufficiently strong signal to distinguish the finest subcellular structures, while protecting the sample from photodamage and bleaching over hours and days of constant imaging.

COS-7 Cell Tomm20 Memerald Calreticulin Tdtomato

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COS-7 cell transiently transfected with Tomm20-mEmerald and Calreticulin-tdTomato. Tomm20 labels the outer membrane of mitochondria, Calreticulin is a protein of the endoplasmic reticulum (ER) where proteins are synthesized. Both are extremely delicate and light-sensitive organelles that are difficult to image with conventional methods. The example shows ER wrapping around mitochondria and assisting mitochondrial fission. Continuous imaging for 43 mins; a total of 301,000 images were recorded.

More research options with a dual-camera configuration

As requested by the research community, the system can now be equipped with two Hamamatsu ORCA-Fusion cameras. This increases the imaging speed and significantly improves multicolor fluorescence experiments.

Two cameras double the temporal resolution at which data is acquired. Researchers can watch delicate processes like mitosis without missing the finest details or the fastest events.

The innovative design of the excitation beam path allows simultaneous excitation of the sample with multiple laser lines. Combined with two cameras, this enables truly simultaneous imaging of two channels – without any time delay. A dual-camera setup also minimizes crosstalk and achieves cleanest results without compromising speed.

The study of dynamic processes in living cells benefits from faster and simultaneous multichannel imaging. Additionally, this new generation of ZEISS Lattice Lightsheet 7 offers, in particular, new possibilities for ratiometric experiments and the investigation of structural co-localization.

U2OS Cells Single Cam and Dual Cam Comparison

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U2OS cells expressing Lifeact-tdTomato and stained with MitoTracker Green. Top row: Single-camera configuration. When optimizing for speed, multi-bandpass filters must be used which can result in some crosstalk (whitish areas). Bottom row: Dual-camera configuration. Crosstalk is minimized. In addition, twice as many images can be acquired, resulting in a doubling of temporal resolution.

About ZEISS

ZEISS is an internationally leading technology enterprise operating in the fields of optics and optoelectronics. In the previous fiscal year, the ZEISS Group generated annual revenue totaling 7.5 billion euros in its four segments Semiconductor Manufacturing Technology, Industrial Quality & Research, Medical Technology and Consumer Markets (status: 30 September 2021).

For its customers, ZEISS develops, produces and distributes highly innovative solutions for industrial metrology and quality assurance, microscopy solutions for the life sciences and materials research, and medical technology solutions for diagnostics and treatment in ophthalmology and microsurgery. The name ZEISS is also synonymous with the world's leading lithography optics, which are used by the chip industry to manufacture semiconductor components. There is global demand for trendsetting ZEISS brand products such as eyeglass lenses, camera lenses and binoculars.

With a portfolio aligned with future growth areas like digitalization, healthcare and Smart Production and a strong brand, ZEISS is shaping the future of technology and constantly advancing the world of optics and related fields with its solutions. The company's significant, sustainable investments in research and development lay the foundation for the success and continued expansion of ZEISS' technology and market leadership. ZEISS invests 13 percent of its revenue in research and development – this high level of expenditure has a long tradition at ZEISS and is also an investment in the future.

With over 35,000 employees, ZEISS is active globally in almost 50 countries with around 30 production sites, 60 sales and service companies and 27 research and development facilities. Founded in 1846 in Jena, the company is headquartered in Oberkochen, Germany. The Carl Zeiss Foundation, one of the largest foundations in Germany committed to the promotion of science, is the sole owner of the holding company, Carl Zeiss AG.

Further information at www.zeiss.com

ZEISS Research Microscopy Solutions

ZEISS Research Microscopy Solutions is the world's only one-stop manufacturer of light, electron, X-ray and ion microscope systems and offers solutions for correlative microscopy. The portfolio comprises of products and services for life sciences, materials and industrial research, as well as education and clinical practice. The unit is headquartered in Jena. Additional production and development sites are located in Oberkochen and Munich, as well as in Cambourne (UK) and Dublin (USA). ZEISS Research Microscopy Solutions is part of the Industrial Quality & Research segment.