BrAInsight Breakthroughs

Advancing Tau-opathy Research in PSP through High-Throughput and Predictive Analytics Dr. Rose B. Creed

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Webinar Information

Discover how high-throughput slide scanning and automated brain atlas registration enable a comprehensive, brain-wide assessment of tau pathology. This holistic platform, incorporating a machine learning model built by MetaCell, reveals predictive links between motor deficits and tau load in specific brain regions—laying the groundwork for uncovering connections between tau pathology and lesser-studied behavioral phenotypes.

Join us for Dr. Creed's webinar to see how this workflow is driving her research forward. After the webinar, dive into educational content designed to help you bring this solution to your lab.

Click here to watch the webinar recording

BRAINSIGHT BREAKTHROUGH WEBINAR

Advancing Tau-opathy Research in PSP through High-Throughput and Predictive Analytics

Presentation by Dr. Rose B. Creed, Univresity of California, San Francisco

Educational resources

Explore our online resources designed to help you recreate the workflows demonstrated.

These include protocols and instructional videos on:

  • Selecting antibodies and immunohistochemistry techniques for identifying tau plaques in the brain​
  • Step-by-step instructions for using the Axioscan 7 to image hundreds of brain section
  • Leveraging arivis Pro to automatically detect tau plaques within neuronal nuclei and cell types​
  • Registering brain sections to the Allen Brain Atlas to assess plaque load by region​
  • Running secondary analysis pipelines to predict animal’s gait patterns based on regional plaque load
  • Harnessing the power of complex datasets and streamlining neuroscience research through collabroative, cloud-based imaging analysis

Towards a mechanistic understanding of how Tau pathology contributes to dysfunction in progressive supranuclear palsy

 
Progressive supranuclear palsy (PSP) is a neurodegenerative disorder with an estimated prevalence of 5-7 people in 100,000. Clinically characterized by impairments in gait, balance, and eye movements, as well as aggregated Tau pathology, PSP leads to death in approximately 5-8 years. No disease-modifying treatments are currently available. The contribution of Tau pathology to the symptoms of patients with PSP is poorly understood, in part due to lack of a rodent model that recapitulates characteristic aspects of PSP. Here, we assessed the hTau. P301S mouse for key clinical features of PSP, finding progressive impairments in balance and gait coordination. Additionally, we found impairments in fast vertical eye movements, one of the most distinctive features of PSP. Across animals, we found that Tau pathology in motor control regions correlated with motor deficits. These findings highlight the utility of the hP301S mouse in modeling key aspects of PSP.
 
 
 
Presenter Rose B. Creed Ph.D. Postdoctoral Fellow, Laboratory of Alexandra Nelson, University of California, San Francisco

Rose B. Creed is a postdoctoral fellow at the University of California, San Francisco, in the laboratory of Alexandra Nelson, M.D., Ph.D. There, she takes a systems neuroscience approach to examine how impairments in the basal ganglia circuit underlie different types of movement disorders. Rose obtained her Bachelor of Science degree in Biology at Stetson university in 2014 and received her Ph.D. from the University of Alabama at Birmingham (UAB) in summer 2020. While at UAB she worked in the labs of Drs. Matthew Goldberg and Lori McMahon, investigating how loss of function of the mitochondrial targeted kinase PINK1 impacts the striatal circuit and its implication for Parkinson’s disease.

COLLABORATOR Zhe (Phil) Dong, Ph.D. Data Scientist, MetaCell

Phil Dong earned his PhD in neuroscience from Mount Sinai School of Medicine, where he focused on calcium imaging data analysis and open-source software development. His expertise spans a wide range of data science techniques applied to systems neuroscience, including electrophysiology, imaging data analysis, behavior segmentation, data mining, visualization, statistical modeling, and artificial neural networks. In addition to his role as a Data Scientist at MetaCell, Phil is also a postdoctoral researcher, where he is working on the development of next-generation miniature microscopes for calcium imaging in behaving animals.

COLLABORATOR Natasha Mayorga BrAInsight Intern, ZEISS Microscopy

Natasha Mayorga is a BrAInsight Intern at ZEISS Microscopy, where she focuses on developing AI-driven workflows for neuroscience microscopy analysis. She works with cross-functional teams to create reproducible image analysis solutions and enhance user experiences with ZEISS arivis software. Natasha is a rising senior at the FAU Harriet L. Wilkes Honors College, majoring in biochemistry and data analytics. Previously, she researched drug-seeking behavior and memory associations at the Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology. Recently, she has incorporated computational techniques into her neuroscience work to bring an interdisciplinary approach to her research.

COLLABORATOR Jason Fung Applications Scientist, Image Analysis, ZEISS Microscopy

Jason Fung is a Software Applications Scientist based at the ZEISS Microscopy Customer Center in the Bay Area. With a Master's in Biomedical Engineering from the University of British Columbia and a background in applied physics and mathematics, Jason has experience coding and building deep learning based analysis tools to handle a multitude of datasets ranging from materials science applications in the clean energy space to analyzing multi-photon images for developmental neuroscience research. As a Software Applications Scientist with a unique blend of experience working on different imaging datasets, Jason provides tailored and innovative image analysis solutions using arivis to help customers answer their most pressing research questions.

COLLABORATOR Stephen Larson, PhD CEO & Co-Founder

Dr. Stephen Larson, co-founder and CEO of MetaCell, is an expert on the intersection between computer technology and biological systems, and is interested in how computer systems can help us understand fundamental principles of life.   Dr. Larson also serves as the Executive Director for the OpenWorm Foundation.   He served as the Chief Information Officer of One Mind for Research, a non-profit dedicated to eradicating brain disease by transforming the healthcare research system through public-private partnerships.   He has worked as a professional software engineer for a major New York City investment bank, co-developed a patent, presented at more than two dozen forums, published in academic journals such as Frontiers in Neuroscience and Nature, and has had his work featured in the New York Times, The Atlantic, Wired, and MSNBC.com.   His diverse educational background includes a Bachelor of Science and Master of Engineering from MIT in Computer Science as well as a PhD in Neuroscience from the University of California, San Diego.

COLLABORATOR Mary Phillips, PhD Business Development Manager, Neuroscience, ZEISS Micoscopy

Mary Phillips, PhD, is a Business Development Manager at ZEISS Microscopy, where she focuses on building strong partnerships within the academic neuroscience community and developing innovative workflows to enable advanced experiments and analysis. Mary earned her PhD in Neuroscience from the University of Alabama at Birmingham and completed her postdoctoral training in Dr. Ryohei Yasuda's laboratory at the Max Planck Florida Institute for Neuroscience. Her research explored the neural circuits underlying social cognition and memory, and she developed an imaging technique capable of distinguishing 10 neuronal subtypes within miniscope calcium recordings. Now, Mary applies her expertise to help drive scientific discovery by bridging cutting-edge imaging technologies with the evolving needs of neuroscientists.

ZEISS & MetaCell Custom Solutions

 
To support Dr. Creed's groundbreaking research on Tau pathology in progressive supranuclear palsy (PSP), ZEISS and MetaCell collaborated to develop a tailored analysis workflow. Leveraging advanced imaging and computational tools, this solution enabled seamless integration of imaging data with powerful analytical insights.
 
Using the ZEISS Axioscan slide scanner, Dr. Creed captured high-resolution images of brain sections. These images were then processed with ZEISS arivis software, which aligned the sections to the Allen Brain Atlas, enabling precise brain region identification. The software also automated Tau plaque detection across the imaged regions, streamlining the quantification of pathology.

Building on this foundation, MetaCell employed machine learning techniques to analyze the correlation between Tau pathology and gait impairments in the hTau.P301S mouse model. Their analysis applied predictive models, such as Lasso regression, to identify key brain regions where Tau pathology was most strongly associated with motor deficits. This approach pinpointed 3-4 critical regions for each gait disorder subtype.

Together, ZEISS and MetaCell deliver cutting-edge analysis solutions that empower researchers to uncover new insights into disease pathology.
 

About MetaCell

MetaCell is a life science-focused software company composed of scientists and software engineers with deep domain expertise in computational neuroscience, molecular biology, data science, and enterprise-grade cloud software development. Their team acts as an innovation partner alongside your R&D team to design and implement novel solutions for aggregating, visualizing, analyzing, and simulating medical and biological data.

Since 2011, MetaCell's solutions and products have been trusted by some of the world's leading academic institutions and largest pharmaceutical companies. From novel research tools to clinical decision support and software for devices, they are a trusted partner to a growing list of academic and industry organizations aiming to innovate and better understand their life science data.

Head to their website to discover MetaCell’s areas of expertise, including data science services, life science data visualization, in-vivo calcium imaging data analysis, AI and machine learning, software development, and a lot more.

  • Email: info@metacell.us
  • LinkedIn: https://www.linkedin.com/company/metacell/
  • X: @MetaCell
  • YouTube: @metacell151
BrAInsights Breakthroughs Series 1

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Through the BrAInsight Breakthroughs, we partner with scientists to drive innovative research and develop advanced workflows. No matter what stage your project is in, reach out to explore how we can collaborate to bring your imaging and analysis goals to life. Together, let’s showcase your breakthroughs and the workflows that made them possible, inspiring and empowering the neuroscience community.