
Low-kV and Variable Pressure Imaging on non-conductive sample for FE-SEM
A ZMCC SkillBuilder Workshop
Workshop Details
Workshop Overview and Agenda
Join us for this virtual workshop designed to boost your skills and confidence in operating ZEISS Gemini column FE-SEMs. In this session, we’ll explore key methods and the underlying theory, with a focus on low Kv and Variable Pressure techniques for imaging insulating samples. We’ll also cover effective strategies to minimize charging artifacts during imaging workflows. While a basic understanding of FE-SEM imaging is helpful, it is not required to attend.
This is a 3-hour virtual workshop that will be streamed live from the ZEISS Microscopy Customer Center (ZMCC) in Dublin, CA. Attendees will be able to view the operation of the instrument via screen-sharing and multiple web cameras and observe the operation of the tool as our FE-SEM expert covers the topics detailed below.
It is recommended that attendees secure time on their instruments after the workshop sessions to apply the shared methods on their own samples. Attendees are strongly encouraged to share their images from individual sessions and interact with our Applications team through independent email threads for further optimization.
Access to a SEM after the workshop is strongly recommended to apply lessons learned and share results with applications team for further support.
Preview the topics covered below and register for the workshop.
Workshop Topics:
- Introduction and basics of FESEM for imaging data acquisition.
- Introduction to Variable Pressure mode and detectors.
- Overview of the workflow required to setup a STEM automated imaging run.
- Setup of a Variable Pressure operation, including parameter optimization.
- Low Kv Imaging for non-conductive samples.
Who should attend?
- New users of ZEISS FE-SEMs.
- Infrequent ZEISS FE-SEMs users in need of a refresh on automated imaging setup and parameter optimization.
- Lab managers, engineers or technicians looking to improve their own skill level.
Learning objectives: after completion of this workshop, attendees should:
- Gain a deeper understanding of Low kV and VP modes, as well as their detectors, to confidently navigate the menus and settings essential for efficient workflow acquisition
- Benefit from reproducible results by improving imaging selections that optimize the quality of non-conductive samples
This is a paid workshop.
Spots are limited.
After the workshop sessions, the attendees are strongly encouraged to attempt the methods shown on their own systems with their own samples. Screen sharing sessions to review the data can be setup with the applications team through independent email threads. Our support team will assist in further optimization and answer any questions to help attendees acquire the best data possible.

David has been working with and promoting SEM and FIB-SEM microscopy solutions since 2017. He joined ZEISS in 2022 as a Product Applications and Sales Specialist at ZEISS-Mexico, covering both EM and XRM systems. Since 2023, David has been part of the ZEISS MRE Applications team at the ZMCC in the Bay Area, responsible for EM applications on EVO C-SEM, Sigma FE-SEM, and Crossbeam FIB-SEM. Prior to joining ZEISS, David served as a dean professor in the School of Medicine at the National Autonomous University of Mexico (UNAM), where he also managed the Microscopy Core Facility. In this role, he collaborated with various research groups and institutions. David's background is in materials science, with a focus on biomaterials and tissue engineering, and he has extensive experience working with scientists and engineers in materials and process characterization, as well as in the industrial applications of SEM systems.
Register for the workshop
This is a paid training. The investment for this 1-day virtual workshop is $400. You will receive a link to complete payment by credit card after signing up. If you require a PO to purchase training please reach out to us after registering.
Once we have received payment, we will send you a confirmation e-mail; expect to receive the link to join the Teams Webinar 1 day prior to training.
To guarantee your spot, please make sure to submit your payment at least 2 days prior.