Digital Slide Scanner

ZEISS Axioscan 7 offers reliably reproducible image quality, no matter if you repeat your imaging task after a day, a week, a month or on a different machine.

Using the new contrast method Transfer of Intensity Equation (TIE) for contrast generation in transparent samples, you record the interaction of a narrow cone of light with your sample’s structures in three images: one in focus, and two out of focus above and below the focal plane. From these three images, the phase information for the central plane is automatically extracted. Continuous acquisition in the z dimension, in combination with flash illumination and GPU-based fast image processing, enables very fast delivery of the final contrast images. You can choose to present this as either phase contrast or DIC-like relief contrast.

TIE contrast is an excellent tool to aid your experiments when working with sensitive fluorescent dyes:

• Detect transparent tissues with little to no contrast in regular brightfield mode.
• Speed the subsequent fluorescence imaging process with very fast flash-based focusing.
• Protect your sensitive dyes from bleaching during focusing by using the lowest light doses.
• Bring your fluorescent labels into context easily by applying the additional contrast information.
  

The UltiMapper I/O PD-L1 kit from Ultivue addresses whether a tumor is “hot” or “cold” and responsive to immune checkpoint inhibition because of a high immune filtrate (hot), in contrast to tumors with low immune infiltrates called “cold tumors” or non-T-cell-inflamed cancers – by exploring multiple cell phenotypes such as cytotoxic immune cells (CD8), immunosuppressive macrophages (Markers CD68, PD-L1) or immune evading tumor cells (Markers CK, PD-L1). The following images represent research content. ZEISS explicitly excludes the possibility of making a diagnosis or recommending treatment for possibly affected patients on the basis of the information generated with the Axioscan 7 spatial biology slide scanner.

Different forms of polarization illumination can be used to highlight different features. Plane polarized light (PPL) shows overall crystal color, habit and pleochroism. Crossed polarized light (XPL) at multiple orientations allows for extinction angle and birefringence to be assessed. Circular polarization allows for maximum birefringence to be observed regardless of grain orientation. All channels are aligned using powerful computational algorithms during acquisition, making resulting data perfect for subsequent segmentation and analysis.