ZEISS LSM 910

LSM Plus improves any confocal experiment with ease, independent of detection mode or emission range. Its linear Wiener filter deconvolution needs next to no manual interaction while still ensuring a reliable quantitative result. The system’s underlying optical property information such as objective lens, refractive index, and emission range is used to automatically adapt processing parameters for best results.

RPE1 cells transfected with H2B-GFP plasmid. Maximum intensity projection of 117 Z-planes. Comparison of without and with LSM Plus.

^{Courtesy of Tingsheng, Mitosis Lab, Singapore}

To truly capture the essence of biological processes, imaging must be done in 4D, as both volume and time are essential for investigating living systems. Lightfield 4D offers a unique solution by imaging an entire volume at an exact point in time, without any time delay.

Transgenic, 3-day old Arabidopsis thaliana hypocotyl (stem) tagged with a mobile photoreceptor-regulated protein labelled in GFP. Video shows 5 minutes of 50 ms exposures taken every 200 ms, acquired upon initial blue-light stimulation.

^{Courtesy of Hannah Walters, Cellular Analysis Facility, MVLS-Shared Research Facilities, University of Glasgow. Data acquired at the Cellular Analysis Facility, University of Glasgow, UK.}

The Microscopy Copilot, your personal AI assistant, helps you to interactively discover new possibilities for your imaging experiments. Ask questions when they are relevant to your current work. Reduce training time by getting new information straight away. Constantly evolve your research and exploit the potential of your specific LSM system configuration.

Neurons and astrocyte in thick brain sections. Different imaging modalities and data processing combined in one project using the Connect toolkit: Widefield overview for ROI definition; fast volume acquisition with ZEISS Lightfield 4D; sensitive super-resolution imaging with ZEISS Airyscan in Multiplex mode; Airyscan jDCV processing; 3D visualization with ZEISS arivis Pro.

^{Sample courtesy of Luisa Cortes, Microscopy Imaging Center of Coimbra, CNC, University of Coimbra, Portugal}

Flow of GFP-labelled hemocytes (insect blood cells) in the hemolymph of Drosophila melanogaster white pre-pupae. ZEISS Lightfield 4D in combination with ZEISS arivis Pro offers the unique opportunity to image and analyze the blood flow under physiological in vivo conditions. Lightfield 4D with its unparalleled speed of 80 volumes per second allows to image a large volume fast enough to follow this process. The 3D algorithms of arivis Pro then allow to segment and track the blood cells throughout a timelapse.

^{Sample courtesy of Iwan Robert Evans, University of Sheffield, UK. Data acquired at Wolfson Light Microscopy Facility in the School of Biosciences at the University of Sheffield.}