ZEISS LSM 990 Spectral Multiplex

LSM 990 Spectral Multiplex offers unparalleled productivity for your demanding spectral imaging experiments, covering a wavelength range from 380 to 900 nm. The intelligent design choices of the beam path eliminate compromises related to spectral separation, sensitivity, speed, signal-to-noise ratio, and resolution. With the direct Lambda track acquisition, you can simultaneously separate 10 or more individual labels. Capturing all spectra in a single scan enables instantaneous separation and real-time display of the resulting channels. This allows you to identify numerous labels in the shortest time, which is useful when enhanced spatial resolution is needed, or for imaging large volumes or live samples. Unwanted signals, such as autofluorescence, can be easily eliminated without inadvertently removing true signal from the target proteins. All Lambda information is preserved, and spectra are displayed in easy-to-read graphs to help you evaluate if you are imaging the expected fluorescent labels.

When you initiate a multi-color experiment, Smart Setup offers excitation and emission data for a wide range of fluorophores. Adjust the entire system to your requirements with a single click by choosing between configuration options for optimum spectral separation, maximum speed, or a balanced compromise. Alternatively, select the hassle-free Lambda Scan mode to capture all relevant signals of the desired spectral range in a single scan. All experimental settings can be saved and easily accessed within ZEN, facilitating quicker utilization of personal experiment configurations. Integrate the LSM Plus feature to ensure optimal signal-to-noise ratio and improved spatial resolution without slowing down your experiment.

When imaging multiple channels or utilizing Lambda mode, the option for spectral unmixing is always available. Previously saved spectra can be retrieved from a local database, and alongside this information, all critical imaging settings are stored and displayed. You can manually select pixels containing spectral information from the newly acquired image, or utilize the built-in Automatic Component Extraction to identify such pixels. These sources of information can be combined in a Linear Unmixing process. Each resulting multi-channel image can undergo validation and quality control, with an optional ‘residual’ channel saved alongside the original data for seamless record-keeping of the experiment. Perform on-the-fly Linear Unmixing while capturing spectral information in single scans through Online Fingerprinting, leading immediately to separated signals – ideal for large volumes and screening specific combinations of fluorescent labels.

Combine all available spectral data acquisition methods, including Lambda Scans, Linear Unmixing, and LSM Plus for SNR enhancement, into one processing pipeline that executes all steps of multi-dimensional experiments. Automated workflows for spectral multiplexing that involve multiple rounds of staining and imaging can be simplified with automated liquid delivery systems. The individual staining, imaging, bleaching, and stripping rounds can be organized within ZEN.¹ Transfer the resulting data to ZEISS arivis pro for 3D registration of spectral multiplexing data, AI object segmentation, or statistical analyses, such as cell neighborhood and dimensionality reduction analyses.