![小鼠大脑,使用脂质染料染色以标记脉管系统膜,使用iDISCO+进行透明化处理,并使用激光片层扫描显微系统成像。样品由美国哈佛大学的E. Diel提供 小鼠大脑,使用脂质染料染色以标记脉管系统膜,使用iDISCO+进行透明化处理,并使用激光片层扫描显微系统成像。样品由美国哈佛大学的E. Diel提供]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-vasculature.jpg/_jcr_content/renditions/original.image_file.100.100.164,185,1714,1735.file/brain-vasculature.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-vasculature.jpg/_jcr_content/renditions/original.image_file.360.360.164,185,1714,1735.file/brain-vasculature.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-vasculature.jpg/_jcr_content/renditions/original.image_file.768.768.164,185,1714,1735.file/brain-vasculature.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-vasculature.jpg/_jcr_content/renditions/original.image_file.1024.1024.164,185,1714,1735.file/brain-vasculature.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-vasculature.jpg/_jcr_content/renditions/original.image_file.1280.1280.164,185,1714,1735.file/brain-vasculature.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-vasculature.jpg/_jcr_content/renditions/original.image_file.1440.1440.164,185,1714,1735.file/brain-vasculature.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-vasculature.jpg/_jcr_content/renditions/original.image_file.1550.1550.164,185,1714,1735.file/brain-vasculature.jpg"})
化学透明化的大脑组织和厚组织
深入研究样品
随着光学组织透明化技术的兴起,大型样品(例如全脑、器官或大型模式生物)能够以细胞级到亚细胞级分辨率进行成像,而无需传统的物理切片。这一发展对于从事解剖学、脉管系统、神经网络或连接组学方面研究的人员来说至关重要。
通过将光透方法与计算能力、数据存储容量、智能渲染和分析算法以及合适的显微技术相结合,可以对这些大型样品进行高效分析。
![通过iDISCO进行透明化处理的小鼠肾脏,使用激光片层扫描显微系统成像。样品由丹麦Gubra公司的U. Roostalu提供 通过iDISCO进行透明化处理的小鼠肾脏,使用激光片层扫描显微系统成像。样品由丹麦Gubra公司的U. Roostalu提供]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.100.67.0,0,1917,1280.file/mouse-kidney-eci-gubra-panel.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.360.240.0,0,1917,1280.file/mouse-kidney-eci-gubra-panel.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.768.512.0,0,1917,1280.file/mouse-kidney-eci-gubra-panel.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.1024.683.0,0,1917,1280.file/mouse-kidney-eci-gubra-panel.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.1280.853.0,0,1917,1280.file/mouse-kidney-eci-gubra-panel.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.1440.960.0,0,1917,1280.file/mouse-kidney-eci-gubra-panel.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.1917.1278.0,0,1917,1280.file/mouse-kidney-eci-gubra-panel.jpg"})
![通过iDISCO进行透明化处理的小鼠肾脏,使用激光片层扫描显微系统成像。样品由丹麦Gubra公司的U. Roostalu提供 通过iDISCO进行透明化处理的小鼠肾脏,使用激光片层扫描显微系统成像。样品由丹麦Gubra公司的U. Roostalu提供]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.100.67.file/mouse-kidney-eci-gubra-panel.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.360.240.file/mouse-kidney-eci-gubra-panel.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.768.512.file/mouse-kidney-eci-gubra-panel.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.1024.683.file/mouse-kidney-eci-gubra-panel.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.1280.853.file/mouse-kidney-eci-gubra-panel.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original.image_file.1440.960.file/mouse-kidney-eci-gubra-panel.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/mouse-kidney-eci-gubra-panel.jpg/_jcr_content/renditions/original./mouse-kidney-eci-gubra-panel.jpg"})
通过iDISCO进行透明化处理的小鼠肾脏,使用激光片层扫描显微系统成像。样品由丹麦Gubra公司的U. Roostalu提供
通过iDISCO进行透明化处理的小鼠肾脏,使用激光片层扫描显微系统成像。样品由丹麦Gubra公司的U. Roostalu提供
使用激光片层扫描显微系统平衡样品尺寸、高分辨率成像和快速采集速度
光片荧光显微技术(LSFM)使用特殊的照明方法,一次即可激发并采集整个平面的数据。再借助透明化的样品,便能够以高速度和高分辨率对大型样品进行数据采集。蔡司Lightsheet 7使您能够对经过化学透明化处理的大脑、器官或大型组织切片进行快速成像。
![小鼠大脑,通过CLARITY方法进行透明化处理 小鼠大脑,通过CLARITY方法进行透明化处理]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.100.67.0,0,1591,1062.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.360.240.0,0,1591,1062.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.768.512.0,0,1591,1062.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.1024.683.0,0,1591,1062.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.1280.853.0,0,1591,1062.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.1440.960.0,0,1591,1062.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.1591.1061.0,0,1591,1062.file/clearing_mouse-neurons_axio-examiner_lsm.jpg"})
![小鼠大脑,通过CLARITY方法进行透明化处理 小鼠大脑,通过CLARITY方法进行透明化处理]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.100.67.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.360.240.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.768.512.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.1024.683.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.1280.853.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original.image_file.1440.960.file/clearing_mouse-neurons_axio-examiner_lsm.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/clearing_mouse-neurons_axio-examiner_lsm.jpg/_jcr_content/renditions/original./clearing_mouse-neurons_axio-examiner_lsm.jpg"})
小鼠大脑,通过CLARITY方法进行透明化处理,使用共聚焦显微镜成像。样品由德国神经生物学马克斯·普朗克研究所的T. Ruff提供
小鼠大脑,通过CLARITY方法进行透明化处理,使用共聚焦显微镜成像。样品由德国神经生物学马克斯·普朗克研究所的T. Ruff提供
使用共聚焦显微镜获得透明化样品的高分辨率结构细节
对于经过化学透明化处理的大脑、器官或其他组织,共聚焦显微镜能够揭示其深处的微小亚细胞细节和高分辨率结构信息。配备Airyscan的蔡司LSM 9共聚焦系列提供超分辨率功能和多重模式,可提高采集速度,快速对极为精细的结构进行成像。
![经透明化处理的小鼠大脑。右上:全脑概览。 经透明化处理的小鼠大脑。右上:全脑概览。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.100.67.0,0,945,631.file/brain-erturk.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.360.240.0,0,945,631.file/brain-erturk.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.768.512.0,0,945,631.file/brain-erturk.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.945.630.0,0,945,631.file/brain-erturk.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.945.630.0,0,945,631.file/brain-erturk.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.945.630.0,0,945,631.file/brain-erturk.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.945.630.0,0,945,631.file/brain-erturk.jpg"})
![经透明化处理的小鼠大脑。右上:全脑概览。 经透明化处理的小鼠大脑。右上:全脑概览。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.100.67.file/brain-erturk.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.360.240.file/brain-erturk.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original.image_file.768.512.file/brain-erturk.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original./brain-erturk.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original./brain-erturk.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original./brain-erturk.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/imaging-cleared-tissues/brain-erturk.jpg/_jcr_content/renditions/original./brain-erturk.jpg"})
经透明化处理的小鼠大脑。右上:全脑概览。左:大脑区域的最大强度投影(MIP)。右下:MIP插入图的细节图。使用变焦显微镜和用于光学切片的结构化照明成像。样品由德国慕尼黑大学的Erturk实验室提供
经透明化处理的小鼠大脑。右上:全脑概览。左:大脑区域的最大强度投影(MIP)。右下:MIP插入图的细节图。使用变焦显微镜和用于光学切片的结构化照明成像。样品由德国慕尼黑大学的Erturk实验室提供
拥有细胞级分辨率的大视场成像
使用变焦显微技术对经化学透明化处理的大脑或大型组织样品进行成像,可在极大观察视野内实现细胞级的分辨率。蔡司Axio Zoom.V16变焦显微镜可为您提供大预览图,您可以将其放大并观察透明化组织中的单个细胞。Axio Zoom.V16配备蔡司Apotome 3,可添加光学切片和增强结构信息。
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* 本页所示图像仅为研究内容。蔡司明确排除基于Axioscan 7玻片扫描仪生成的信息对可能受影响的患者进行诊断或推荐治疗的可能性。