![高分辨率超微结构成像——使用电子显微镜探索生物样品 高分辨率超微结构成像——使用电子显微镜探索生物样品]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/fe-sem_dendritic-cell_3view_2.jpg/_jcr_content/renditions/original.image_file.100.100.file/fe-sem_dendritic-cell_3view_2.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/fe-sem_dendritic-cell_3view_2.jpg/_jcr_content/renditions/original.image_file.360.360.file/fe-sem_dendritic-cell_3view_2.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/fe-sem_dendritic-cell_3view_2.jpg/_jcr_content/renditions/original.image_file.768.768.file/fe-sem_dendritic-cell_3view_2.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/fe-sem_dendritic-cell_3view_2.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/fe-sem_dendritic-cell_3view_2.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/fe-sem_dendritic-cell_3view_2.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/fe-sem_dendritic-cell_3view_2.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/fe-sem_dendritic-cell_3view_2.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/fe-sem_dendritic-cell_3view_2.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/fe-sem_dendritic-cell_3view_2.jpg/_jcr_content/renditions/original./fe-sem_dendritic-cell_3view_2.jpg"})
高分辨率超微结构成像
使用电子显微镜探索生物样品
您可能正在寻找只有电子显微镜才能达到的极高分辨率,也可能正期待对样品深处的微小结构有三维认知,或者想要更深入地探究原生状态下的超微结构——蔡司电子显微镜为生物样品的高分辨率结构成像提供解决方案,推动了细胞和癌症研究、微生物学和病毒学、免疫学、神经科学、胚胎学或发育生物学乃至植物科学的发展。
![小鼠大脑,OTO染色,使用蔡司GeminiSEM 300成像。样品由瑞士巴塞尔弗雷德里希·米歇尔生物医学研究所(FMI)的C. Genoud提供 小鼠大脑,OTO染色,使用蔡司GeminiSEM 300成像。样品由瑞士巴塞尔弗雷德里希·米歇尔生物医学研究所(FMI)的C. Genoud提供]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.100.56.0,0,1920,1082.file/geminisem-braindetail.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.360.203.0,0,1920,1082.file/geminisem-braindetail.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.768.432.0,0,1920,1082.file/geminisem-braindetail.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.1024.576.0,0,1920,1082.file/geminisem-braindetail.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.1280.720.0,0,1920,1082.file/geminisem-braindetail.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.1440.810.0,0,1920,1082.file/geminisem-braindetail.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.1920.1080.0,0,1920,1082.file/geminisem-braindetail.jpg"})
![小鼠大脑,OTO染色,使用蔡司GeminiSEM 300成像。样品由瑞士巴塞尔弗雷德里希·米歇尔生物医学研究所(FMI)的C. Genoud提供 小鼠大脑,OTO染色,使用蔡司GeminiSEM 300成像。样品由瑞士巴塞尔弗雷德里希·米歇尔生物医学研究所(FMI)的C. Genoud提供]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.100.56.file/geminisem-braindetail.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.360.203.file/geminisem-braindetail.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.768.432.file/geminisem-braindetail.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.1024.576.file/geminisem-braindetail.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.1280.720.file/geminisem-braindetail.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.1440.810.file/geminisem-braindetail.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original./geminisem-braindetail.jpg"})
小鼠大脑,OTO染色,使用蔡司GeminiSEM 300成像。
样品由瑞士巴塞尔弗雷德里希·米歇尔生物医学研究所(FMI)的C. Genoud提供
小鼠大脑,OTO染色,使用蔡司GeminiSEM 300成像。
样品由瑞士巴塞尔弗雷德里希·米歇尔生物医学研究所(FMI)的C. Genoud提供
通过高分辨率成像获取超微结构信息
场发射扫描电子显微镜(包括蔡司GeminiSEM系列和蔡司Sigma系列)可实现分辨率达亚纳米级的高衬度成像。您可以使用场发射扫描电子显微镜体验类似透射电子显微镜的成像效果,观察树脂包埋样品超薄切片中的细胞内结构、细胞器和病毒。
无需预先切片的高分辨率三维成像
高分辨率三维成像可协助从单细胞到大型组织的详细结构研究,包括从连接组学研究到整个生物体的发育生物学研究。
通过将集成在FE-SEM内的切面成像功能与蔡司3View结合使用,可直接在腔室中对样品块进行切片,并在每次切片后成像。该方法允许对更大的三维体积进行研究,最大可达数百万立方微米,Z轴切片厚度可低至15 nm。
此外,您也可使用蔡司Crossbeam的聚焦离子束铣削功能,以获得可高达3 nm的Z轴分辨率,并借助FIB-SEM断层扫描实现超高分辨率三维成像。
![小鼠大脑切片,最高采集速度为1.22千兆像素/秒。 小鼠大脑切片,最高采集速度为1.22千兆像素/秒。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.100.67.0,0,1917,1280.file/multisem-mouse-brain.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.360.240.0,0,1917,1280.file/multisem-mouse-brain.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.768.512.0,0,1917,1280.file/multisem-mouse-brain.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1024.683.0,0,1917,1280.file/multisem-mouse-brain.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1280.853.0,0,1917,1280.file/multisem-mouse-brain.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1440.960.0,0,1917,1280.file/multisem-mouse-brain.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1917.1278.0,0,1917,1280.file/multisem-mouse-brain.jpg"})
![小鼠大脑切片,最高采集速度为1.22千兆像素/秒。 小鼠大脑切片,最高采集速度为1.22千兆像素/秒。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.100.67.file/multisem-mouse-brain.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.360.240.file/multisem-mouse-brain.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.768.512.file/multisem-mouse-brain.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1024.683.file/multisem-mouse-brain.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1280.853.file/multisem-mouse-brain.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1440.960.file/multisem-mouse-brain.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original./multisem-mouse-brain.jpg"})
小鼠大脑切片,最高采集速度为1.22千兆像素/秒。
由美国马萨诸塞州剑桥市哈佛大学的J. Lichtman提供
小鼠大脑切片,最高采集速度为1.22千兆像素/秒。
由美国马萨诸塞州剑桥市哈佛大学的J. Lichtman提供
以极高速度扫描大组织切片的电子显微技术
蔡司开发出一种用于样品大区域成像的新型多光束扫描电子显微镜技术,即蔡司MultiSEM系列。MultiSEM与样品制备机器人协作,通过阵列断层扫描显著提升了三维超分辨率数据的采集速度。现在可实现对更大(1 mm³)的脑组织进行成像(例如用于连接组学分析),也可轻松实现对其他组织体积进行高分辨率成像。
使用低温FE-SEM研究近原生状态下的结构和功能
只有当您冷冻样品而非使用化学固定剂时,才能观察到原生形态。蔡司场发射扫描电子显微镜和聚焦离子束扫描电子显微镜支持低温工作流,可以在低电压下以出色的性能对精细样品进行成像。蔡司还开发出冷冻关联工作流程,通过简单易用的无缝工作流程将宽场、激光扫描以及聚焦离子束扫描电子显微技术关联起来。
![在可变压力模式下用扫描电子显微镜成像的小鼠舌头截面。 在可变压力模式下用扫描电子显微镜成像的小鼠舌头截面。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.100.75.0,0,2048,1536.file/sen_mouse-tongue_evo.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.360.270.0,0,2048,1536.file/sen_mouse-tongue_evo.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.768.576.0,0,2048,1536.file/sen_mouse-tongue_evo.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1024.768.0,0,2048,1536.file/sen_mouse-tongue_evo.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1280.960.0,0,2048,1536.file/sen_mouse-tongue_evo.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1440.1080.0,0,2048,1536.file/sen_mouse-tongue_evo.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1920.1440.0,0,2048,1536.file/sen_mouse-tongue_evo.jpg"})
![在可变压力模式下用扫描电子显微镜成像的小鼠舌头截面。 在可变压力模式下用扫描电子显微镜成像的小鼠舌头截面。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.100.75.file/sen_mouse-tongue_evo.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.360.270.file/sen_mouse-tongue_evo.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.768.576.file/sen_mouse-tongue_evo.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1024.768.file/sen_mouse-tongue_evo.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1280.960.file/sen_mouse-tongue_evo.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1440.1080.file/sen_mouse-tongue_evo.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1920.1440.file/sen_mouse-tongue_evo.jpg"})
在可变压力模式下用扫描电子显微镜成像的小鼠舌头截面。
样品由德国Heinrich Pette研究所的R. Reimer提供
在可变压力模式下用扫描电子显微镜成像的小鼠舌头截面。
样品由德国Heinrich Pette研究所的R. Reimer提供
形貌成像
使用SEM(例如蔡司EVO)或FE-SEM(例如蔡司Sigma),无需喷镀或复杂的样品制备,即可对各种样品的表面形态进行出色成像。您可以对未施加喷镀的昆虫或骨骼材料、湿样品、栅格切片、树脂包埋块面或玻璃化样品进行成像。使用可变压力来控制腔室中的环境条件,并在低真空条件下对样品进行成像。
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* 本页所示图像仅为研究内容。蔡司明确排除基于Axioscan 7玻片扫描仪生成的信息对可能受影响的患者进行诊断或推荐治疗的可能性。