![配备冷冻聚焦离子束扫描电子显微镜的体电镜]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem.jpg/_jcr_content/renditions/original.image_file.100.56.file/cryo-fib-sem.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem.jpg/_jcr_content/renditions/original.image_file.360.203.file/cryo-fib-sem.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem.jpg/_jcr_content/renditions/original.image_file.768.432.file/cryo-fib-sem.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem.jpg/_jcr_content/renditions/original.image_file.1024.576.file/cryo-fib-sem.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem.jpg/_jcr_content/renditions/original.image_file.1280.720.file/cryo-fib-sem.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem.jpg/_jcr_content/renditions/original.image_file.1440.810.file/cryo-fib-sem.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem.jpg/_jcr_content/renditions/original./cryo-fib-sem.jpg"} "配备冷冻聚焦离子束扫描电子显微镜的体电镜")
体电镜技术
冷冻聚焦离子束扫描电镜
轻松捕捉近原生状态超高分辨率细胞动态快照
典型工作流程示意图
![冷冻聚焦离子束扫描电子显微镜切割]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-milling.png/_jcr_content/renditions/original.image_file.100.100.0,1,1280,1281.file/cryo-fib-sem-milling.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-milling.png/_jcr_content/renditions/original.image_file.360.360.0,1,1280,1281.file/cryo-fib-sem-milling.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-milling.png/_jcr_content/renditions/original.image_file.768.768.0,1,1280,1281.file/cryo-fib-sem-milling.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-milling.png/_jcr_content/renditions/original.image_file.1024.1024.0,1,1280,1281.file/cryo-fib-sem-milling.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/cryo-fib-sem-milling.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/cryo-fib-sem-milling.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/cryo-fib-sem-milling.png"} "冷冻聚焦离子束扫描电子显微镜切割")
1
用聚焦离子束在玻璃化、未染色的样品上切割出沟槽，直到看到感兴趣的结构。
![冷冻聚焦离子束扫描电子显微镜图像采集]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.100.100.0,1,1280,1281.file/cryo-fib-sem-image-acquisition.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.360.360.0,1,1280,1281.file/cryo-fib-sem-image-acquisition.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.768.768.0,1,1280,1281.file/cryo-fib-sem-image-acquisition.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1024.1024.0,1,1280,1281.file/cryo-fib-sem-image-acquisition.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/cryo-fib-sem-image-acquisition.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/cryo-fib-sem-image-acquisition.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/cryo-fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/cryo-fib-sem-image-acquisition.png"} "冷冻聚焦离子束扫描电子显微镜图像采集")
2
针对新暴露的样品表面的感兴趣区域结构进行成像。重复进行切割和成像过程，直至完全获得感兴趣区域结构的图像。样品在整个过程中保持玻璃化状态。
![处理和分割]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-processing-segmentation.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-processing-segmentation.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-processing-segmentation.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-processing-segmentation.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png"} "处理和分割")
3
对采集的电镜图像进行处理和对齐形成三维数据集。细胞区室可以被识别和分割。
![三维可视化分析]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-3d-visualization-analysis.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-3d-visualization-analysis.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-3d-visualization-analysis.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-3d-visualization-analysis.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png"} "三维可视化分析")
4
可对经过分割的三维数据集进行可视化、研究和统计分析。
应用案例
了解颗石藻中方解石晶体的生物矿化过程
德国波茨坦-戈尔姆马克斯·普朗克植物生理学研究所的S. Sviben和A. Scheffel以及德国波茨坦-戈尔姆马克斯·普朗克胶体与界面研究所的L. Bertinetti
赫氏颗石藻中颗石的形成
方解石颗粒及其超微结构环境实现可视化
使用传统的水基制备方案难以对颗石藻的可溶性无定形钙相超微结构环境进行成像。在冷冻条件下工作的聚焦离子束扫描电子显微镜，可以对接近原生状态的玻璃化海藻进行成像，从而以三维方式揭示超微结构。
赫氏颗石藻细胞被高压冷冻。在成像过程中样品保持冷冻状态；使用蔡司Crossbeam在冷冻条件下采集聚焦离子束扫描电子显微镜数据集。三维重构显示了成熟的颗石藻（黄色），处于初生状态的颗石藻（蓝色）以及脂质体（红色）。
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/application-examples/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg/_jcr_content/renditions/original.image_file.100.56.0,50,1600,950.file/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/application-examples/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg/_jcr_content/renditions/original.image_file.360.203.0,50,1600,950.file/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/application-examples/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg/_jcr_content/renditions/original.image_file.768.432.0,50,1600,950.file/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/application-examples/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg/_jcr_content/renditions/original.image_file.1024.576.0,50,1600,950.file/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/application-examples/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg/_jcr_content/renditions/original.image_file.1280.720.0,50,1600,950.file/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/application-examples/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg/_jcr_content/renditions/original.image_file.1440.810.0,50,1600,950.file/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/cryo-fib-sem/application-examples/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg/_jcr_content/renditions/original.image_file.1600.900.0,50,1600,950.file/zeiss-microscopy-cryo-fib-sem_adenacarcinoma-cells-grow-on-saphire-discs.jpg"})
![蔡司Crossbeam——专为高效率三维分析和样品制备量身打造的FIB-SEM]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.100.100.file/crossbeam-550-system.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.360.360.file/crossbeam-550-system.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.768.768.file/crossbeam-550-system.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/crossbeam-550-system.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/crossbeam-550-system.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/crossbeam-550-system.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original./crossbeam-550-system.jpg"} "蔡司Crossbeam")
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![蔡司冷冻关联工作流程]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.100.100.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.360.360.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.768.768.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.1024.1024.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.1280.1280.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.1280.1280.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.1280.1280.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg"} "蔡司冷冻关联工作流程")