![配备聚焦离子束扫描电子显微镜的体电镜]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.100.56.file/fib-sem.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.360.203.file/fib-sem.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.768.432.file/fib-sem.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.1024.576.file/fib-sem.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.1280.720.file/fib-sem.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original.image_file.1440.810.file/fib-sem.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem.jpg/_jcr_content/renditions/original./fib-sem.jpg"} "配备聚焦离子束扫描电子显微镜的体电镜")
体电镜技术
聚焦离子束扫描电子显微镜
使用高分辨率、各向同性体积数据实现精准三维重构
典型工作流程示意图
![聚焦离子束扫描电子显微镜切割]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.100.100.0,1,1280,1281.file/fib-sem-milling.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.360.360.0,1,1280,1281.file/fib-sem-milling.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.768.768.0,1,1280,1281.file/fib-sem-milling.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.1024.1024.0,1,1280,1281.file/fib-sem-milling.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-milling.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-milling.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-milling.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-milling.png"} "聚焦离子束扫描电子显微镜切割")
1
用聚焦离子束在树脂包埋的样品上切割出沟槽，直到看到感兴趣的结构。
![聚焦离子束扫描电子显微镜采集图像]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.100.100.0,1,1280,1281.file/fib-sem-image-acquisition.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.360.360.0,1,1280,1281.file/fib-sem-image-acquisition.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.768.768.0,1,1280,1281.file/fib-sem-image-acquisition.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1024.1024.0,1,1280,1281.file/fib-sem-image-acquisition.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-image-acquisition.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-image-acquisition.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem-image-acquisition.png/_jcr_content/renditions/original.image_file.1280.1280.0,1,1280,1281.file/fib-sem-image-acquisition.png"} "聚焦离子束扫描电子显微镜采集图像")
2
针对新暴露的样品表面的感兴趣区域结构进行成像。重复进行切割和成像过程，直至完全获得感兴趣区域结构的图像。
![处理和分割]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-processing-segmentation.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-processing-segmentation.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-processing-segmentation.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-processing-segmentation.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png"} "处理和分割")
3
对采集的电镜图像进行处理和对齐形成三维数据集。细胞区室可以被识别和分割。
![三维可视化分析]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-3d-visualization-analysis.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-3d-visualization-analysis.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-3d-visualization-analysis.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-3d-visualization-analysis.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png"} "三维可视化分析")
4
可对经过分割的三维数据集进行可视化、研究和统计分析。
应用实例
细胞超微结构的三维高分辨率、各向同性成像
数据集由德国海德堡EMBL的Anna Steyer和Yannick Schwab友情提供
海拉细胞的三维成像
使用蔡司聚焦离子束扫描电子显微镜技术进行自动三维连续成像
使用聚焦离子束依次去除样品8 nm厚的表面层，并使用扫描电子显微镜扫描暴露的体表面，从而获得高分辨率的三维体积图像。 在arivis Pro中使用arivis Cloud训练的深度学习模型对细胞成分进行自动分割和成像，以实现对不同细胞成分的可视化和量化分析。
![基于聚焦离子束切割的原始数据，对藻类高尔基体进行三维重构]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.100.75.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.360.270.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.768.576.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.1024.768.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.1280.960.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.1280.960.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/fib-sem/fib-sem_golgi-body_crossbeam_1.jpg/_jcr_content/renditions/original.image_file.1280.960.0,160,1280,1120.file/fib-sem_golgi-body_crossbeam_1.jpg"} "基于聚焦离子束切割的原始数据，对藻类高尔基体进行三维重构")
图像由英国牛津布鲁克斯大学的L. Hughes博士提供
表征高尔基体
洞悉高尔基体在蛋白质修饰和运输中的作用
该图显示了来自聚焦离子束扫描电子显微镜数据集的藻类高尔基体的三维重构。该数据集区分高尔基体的顺面与反面（黄色/红色：高尔基体顺面，紫色/蓝色：高尔基体反面）。对使用蔡司Crossbeam聚焦离子束扫描电子显微镜技术采集的高分辨率数据集中的细胞成分进行分割，由此可确保内部成分的准确表征和量化分析。
![蔡司Crossbeam——专为高效率三维分析和样品制备量身打造的FIB-SEM]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.100.100.file/crossbeam-550-system.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.360.360.file/crossbeam-550-system.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.768.768.file/crossbeam-550-system.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/crossbeam-550-system.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/crossbeam-550-system.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/crossbeam-550-system.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original./crossbeam-550-system.jpg"} "蔡司Crossbeam")
![桌面显示器显示蔡司arivis Cloud软件的屏幕，其中包含内皮细胞的分割预测。每个细胞作为一个单独的对象进行检测。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original.image_file.100.100.file/zeiss-arivis-cloud-product-0724.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original.image_file.360.360.file/zeiss-arivis-cloud-product-0724.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original.image_file.768.768.file/zeiss-arivis-cloud-product-0724.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss-arivis-cloud-product-0724.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original./zeiss-arivis-cloud-product-0724.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original./zeiss-arivis-cloud-product-0724.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-cloud/zeiss-arivis-cloud-product-0724.jpg/_jcr_content/renditions/original./zeiss-arivis-cloud-product-0724.jpg"} "蔡司arivis Cloud软件")
![桌面显示器显示蔡司arivis Pro软件的屏幕，其中包含聚焦离子束扫描电子显微镜体电镜数据核孔复合体的分析结果。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.100.100.file/zeiss-arivis-pro-product-0724.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.360.360.file/zeiss-arivis-pro-product-0724.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.768.768.file/zeiss-arivis-pro-product-0724.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss-arivis-pro-product-0724.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original./zeiss-arivis-pro-product-0724.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original./zeiss-arivis-pro-product-0724.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/arivis-vision4d/zeiss-arivis-pro-product-0724.png/_jcr_content/renditions/original./zeiss-arivis-pro-product-0724.png"} "蔡司arivis Pro软件")