![配备多光束Array Tomography的体电镜 配备多光束Array Tomography的体电镜]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography.jpg/_jcr_content/renditions/original.image_file.100.56.file/multibeam-array-tomography.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography.jpg/_jcr_content/renditions/original.image_file.360.203.file/multibeam-array-tomography.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography.jpg/_jcr_content/renditions/original.image_file.768.432.file/multibeam-array-tomography.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography.jpg/_jcr_content/renditions/original.image_file.1024.576.file/multibeam-array-tomography.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography.jpg/_jcr_content/renditions/original.image_file.1280.720.file/multibeam-array-tomography.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography.jpg/_jcr_content/renditions/original.image_file.1440.810.file/multibeam-array-tomography.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography.jpg/_jcr_content/renditions/original./multibeam-array-tomography.jpg"})
体电镜技术
多光束Array Tomography
高效采集大量超微结构数据
典型工作流程示意图
![多光束Array Tomography的样品制备 多光束Array Tomography的样品制备]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-sample-preparation.png/_jcr_content/renditions/original.image_file.100.100.file/multibeam-array-tomography-sample-preparation.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-sample-preparation.png/_jcr_content/renditions/original.image_file.360.360.file/multibeam-array-tomography-sample-preparation.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-sample-preparation.png/_jcr_content/renditions/original.image_file.768.768.file/multibeam-array-tomography-sample-preparation.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-sample-preparation.png/_jcr_content/renditions/original.image_file.1024.1024.file/multibeam-array-tomography-sample-preparation.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-sample-preparation.png/_jcr_content/renditions/original./multibeam-array-tomography-sample-preparation.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-sample-preparation.png/_jcr_content/renditions/original./multibeam-array-tomography-sample-preparation.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-sample-preparation.png/_jcr_content/renditions/original./multibeam-array-tomography-sample-preparation.png"})
1
将树脂包埋的样品切成一系列连续切片,每个切片的厚度通常为30–70 nm,并按切割顺序将切片置于样品台上。
![多光束Array Tomography的图像采集 多光束Array Tomography的图像采集]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-image-acquisition.png/_jcr_content/renditions/original.image_file.100.100.file/multibeam-array-tomography-image-acquisition.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-image-acquisition.png/_jcr_content/renditions/original.image_file.360.360.file/multibeam-array-tomography-image-acquisition.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-image-acquisition.png/_jcr_content/renditions/original.image_file.768.768.file/multibeam-array-tomography-image-acquisition.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-image-acquisition.png/_jcr_content/renditions/original.image_file.1024.1024.file/multibeam-array-tomography-image-acquisition.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-image-acquisition.png/_jcr_content/renditions/original./multibeam-array-tomography-image-acquisition.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-image-acquisition.png/_jcr_content/renditions/original./multibeam-array-tomography-image-acquisition.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-image-acquisition.png/_jcr_content/renditions/original./multibeam-array-tomography-image-acquisition.png"})
2
每个连续切片均在多光束扫描电子显微镜(蔡司MultiSEM)中成像。
![处理和分割 处理和分割]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-processing-segmentation.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-processing-segmentation.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-processing-segmentation.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-processing-segmentation.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png"})
3
对采集的电镜图像进行处理和对齐形成三维数据集。细胞区室可以被识别和分割。
![三维可视化分析 三维可视化分析]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-3d-visualization-analysis.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-3d-visualization-analysis.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-3d-visualization-analysis.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-3d-visualization-analysis.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png"})
4
可对经过分割的三维数据集进行可视化、研究和统计分析。
应用实例
以更大尺度了解大脑组织的神经元连接
![30 nm厚大脑切片 在6.5分钟内对30 nm厚大脑切片采集的1平方毫米的拼图图块,像素大小4 nm,切片使用高衬度染色方案制备,并使用ATUMtome(一种将切片收集在胶带上的超薄切片机)进行切片。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.100.100.file/multi-beam-mouse-brain.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.360.360.file/multi-beam-mouse-brain.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.768.768.file/multi-beam-mouse-brain.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain.jpg"})
30 nm厚大脑切片
![30 nm厚大脑切片 在6.5分钟内对30 nm厚大脑切片采集的1平方毫米的拼图图块,像素大小4 nm,切片使用高衬度染色方案制备,并使用ATUMtome(一种将切片收集在胶带上的超薄切片机)进行切片。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.100.100.file/maginfication_multi-beam-mouse-brain.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.360.360.file/maginfication_multi-beam-mouse-brain.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.768.768.file/maginfication_multi-beam-mouse-brain.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/maginfication_multi-beam-mouse-brain.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/maginfication_multi-beam-mouse-brain.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/maginfication_multi-beam-mouse-brain.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1920.1920.file/maginfication_multi-beam-mouse-brain.jpg"})
在6.5分钟内对30 nm厚大脑切片采集的1平方毫米的拼图图块,像素大小4 nm,切片使用高衬度染色方案制备,并使用ATUMtome(一种将切片收集在胶带上的超薄切片机)进行切片。
在6.5分钟内对30 nm厚大脑切片采集的1平方毫米的拼图图块,像素大小4 nm,切片使用高衬度染色方案制备,并使用ATUMtome(一种将切片收集在胶带上的超薄切片机)进行切片。
![单个六边形多光束观察视野(mFoV) 从前一个数据集中提取的七个典型的mFoV拼接成的单个六边形多光束观察视野(mFoV)。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.100.100.file/multi-beam-mouse-brain-mfov.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.360.360.file/multi-beam-mouse-brain-mfov.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.768.768.file/multi-beam-mouse-brain-mfov.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-mfov.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-mfov.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-mfov.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-mfov.jpg"})
![单个六边形多光束观察视野(mFoV) 从前一个数据集中提取的七个典型的mFoV拼接成的单个六边形多光束观察视野(mFoV)。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.100.100.file/maginfication_multi-beam-mouse-brain-mfov.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.360.360.file/maginfication_multi-beam-mouse-brain-mfov.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.768.768.file/maginfication_multi-beam-mouse-brain-mfov.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/maginfication_multi-beam-mouse-brain-mfov.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/maginfication_multi-beam-mouse-brain-mfov.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/maginfication_multi-beam-mouse-brain-mfov.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.1920.1920.file/maginfication_multi-beam-mouse-brain-mfov.jpg"})
从前一个数据集中提取的七个典型的mFoV拼接成的单个六边形多光束观察视野(mFoV)。
从前一个数据集中提取的七个典型的mFoV拼接成的单个六边形多光束观察视野(mFoV)。
![单个mFoV,由61个单独图块组成 单个mFoV示例,由使用61条并行电子束采集的61个单独图块组成,从左到右覆盖范围超过100 µm,通常在数秒内就可完成采集。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.100.100.file/multi-beam-mouse-brain-single-mfov.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.360.360.file/multi-beam-mouse-brain-single-mfov.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.768.768.file/multi-beam-mouse-brain-single-mfov.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-single-mfov.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-single-mfov.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-single-mfov.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-single-mfov.jpg"})
![单个mFoV,由61个单独图块组成 单个mFoV示例,由使用61条并行电子束采集的61个单独图块组成,从左到右覆盖范围超过100 µm,通常在数秒内就可完成采集。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.100.100.file/maginfication_multi-beam-mouse-brain-single-mfov.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.360.360.file/maginfication_multi-beam-mouse-brain-single-mfov.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.768.768.file/maginfication_multi-beam-mouse-brain-single-mfov.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/maginfication_multi-beam-mouse-brain-single-mfov.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/maginfication_multi-beam-mouse-brain-single-mfov.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/maginfication_multi-beam-mouse-brain-single-mfov.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.1920.1920.file/maginfication_multi-beam-mouse-brain-single-mfov.jpg"})
单个mFoV示例,由使用61条并行电子束采集的61个单独图块组成,从左到右覆盖范围超过100 µm,通常在数秒内就可完成采集。