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用于生殖医学的显微镜应用
用于受精的显微镜解决方案
为所需生育治疗制备的卵母细胞和精子
经过质量评估后,对卵母细胞和精子进行制备,用于所需的生育治疗。体外受精(IVF)、胞浆内单精子注射(ICSI)和胞浆内形态选择精子注射(IMSI)是用于人类和动物生育治疗的生殖技术。特别是胞浆内单精子注射和胞浆内形态选择精子注射,需要配备显微操作组件的高分辨率和高衬度显微镜。该过程通常在置于加热型显微镜载物台玻璃衬管的培养皿中进行。胞浆内单精子注射的受精概率高于体外受精。因此,在许多国家/地区,ICSI是首选的辅助生殖技术。
用于生育治疗的生殖技术
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体外受精(IVF)
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胞浆内单精子注射(ICSI) |
胞浆内形态选择精子注射(IMSI)
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采集和孵化卵子
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采集和孵化卵子
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采集和孵化卵子
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添加未经筛选的精子细胞
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使用固定针稳定卵母细胞
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使用倒置显微镜对健康卵子进行形态选择
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将两者混合(体外)数天
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使用玻璃微针采集单个精子
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使用固定针稳定卵母细胞
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健康、移动的精子细胞主动使卵子受精
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通过使用微针头切断未经筛选的精子细胞尾来固定精子细胞
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使用正置显微镜进行精液分析
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将胚胎转移到子宫
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卵母细胞穿过细胞膜(卵膜),精子被引导至卵母细胞的内部(细胞质)
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使用具有高放大倍率、DIC观察方式和油浸物镜的倒置显微镜对健康精子细胞进行形态选择
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然后将精子释放到卵母细胞中
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通过使用玻璃微针切断经过筛选的精子尾来固定精子
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确保卵细胞的透明带和极体等细胞结构清晰可见
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卵母细胞穿过细胞膜(卵膜),精子被引导至卵母细胞的内部(细胞质)
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然后将精子释放到卵母细胞中
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确保卵细胞的透明带和极体等细胞结构清晰可见
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必须评估精子细胞的形状和液泡数
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应用图像
Axio Observer,Narishige显微操作系统
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Axio Observer,Narishige显微操作系统
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