![蔡司Axio Observer 7的自动加水模块]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_stage.jpg/_jcr_content/renditions/original.image_file.100.100.file/autoimmersion-module_stage.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_stage.jpg/_jcr_content/renditions/original.image_file.360.360.file/autoimmersion-module_stage.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_stage.jpg/_jcr_content/renditions/original.image_file.768.768.file/autoimmersion-module_stage.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_stage.jpg/_jcr_content/renditions/original./autoimmersion-module_stage.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_stage.jpg/_jcr_content/renditions/original./autoimmersion-module_stage.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_stage.jpg/_jcr_content/renditions/original./autoimmersion-module_stage.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_stage.jpg/_jcr_content/renditions/original./autoimmersion-module_stage.jpg"} "蔡司Axio Observer 7的自动加水模块")
蔡司Axio Observer 7的自动加水模块
自始至终实现可靠的数据采集
要实现高分辨率成像，样品与物镜之间需要有介质。手动加水可能会造成数据点丢失，甚至因用户操作失误导致显微镜损坏，而且不够简单高效。蔡司Axio Observer 7的宽场和共聚焦系统带有自动加水模块，是您维持水镜介质的自动化、易用的解决方案。
![减少对您实验和显微镜的风险]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit1.jpg/_jcr_content/renditions/original.image_file.100.50.file/autoimmersion-module_benefit1.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit1.jpg/_jcr_content/renditions/original.image_file.360.180.file/autoimmersion-module_benefit1.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit1.jpg/_jcr_content/renditions/original.image_file.768.384.file/autoimmersion-module_benefit1.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit1.jpg/_jcr_content/renditions/original.image_file.1024.512.file/autoimmersion-module_benefit1.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit1.jpg/_jcr_content/renditions/original.image_file.1280.640.file/autoimmersion-module_benefit1.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit1.jpg/_jcr_content/renditions/original.image_file.1440.720.file/autoimmersion-module_benefit1.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit1.jpg/_jcr_content/renditions/original./autoimmersion-module_benefit1.jpg"} "减少对您实验和显微镜的风险")
减少对您实验和显微镜的风险
该模块为水镜自动应用介质。水被直接泵入样品和物镜之间，每次应用仅使用几微升，内置的水保护系统保证操作安全。在保持物镜焦点和位置的情况下应用介质，确保实验不受干扰，可避免用户在手动应用介质时常犯的错误。
![一如既往的强大功能性]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit2.jpg/_jcr_content/renditions/original.image_file.100.50.file/autoimmersion-module_benefit2.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit2.jpg/_jcr_content/renditions/original.image_file.360.180.file/autoimmersion-module_benefit2.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit2.jpg/_jcr_content/renditions/original.image_file.768.384.file/autoimmersion-module_benefit2.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit2.jpg/_jcr_content/renditions/original.image_file.1024.512.file/autoimmersion-module_benefit2.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit2.jpg/_jcr_content/renditions/original.image_file.1280.640.file/autoimmersion-module_benefit2.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit2.jpg/_jcr_content/renditions/original.image_file.1440.720.file/autoimmersion-module_benefit2.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit2.jpg/_jcr_content/renditions/original./autoimmersion-module_benefit2.jpg"} "一如既往的强大功能性")
一如既往的强大功能性
自动加水模块易于安装且与众多蔡司水镜兼容。即使存在难以处理的配置，如显微镜带有一个不透明XL培养装置，安装工作依然十分简单。无需任何工具即可在物镜之间移动组件。移液器可在几秒钟内在物镜间切换，物镜功能不会受到影响。该模块不会缩短工作距离，也不会影响可见度或校正环的使用。
![全面的软件集成，操作简单直观]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit3.jpg/_jcr_content/renditions/original.image_file.100.50.file/autoimmersion-module_benefit3.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit3.jpg/_jcr_content/renditions/original.image_file.360.180.file/autoimmersion-module_benefit3.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit3.jpg/_jcr_content/renditions/original.image_file.768.384.file/autoimmersion-module_benefit3.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit3.jpg/_jcr_content/renditions/original.image_file.1024.512.file/autoimmersion-module_benefit3.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit3.jpg/_jcr_content/renditions/original.image_file.1280.640.file/autoimmersion-module_benefit3.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit3.jpg/_jcr_content/renditions/original.image_file.1440.720.file/autoimmersion-module_benefit3.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/autoimmersion-module_benefit3.jpg/_jcr_content/renditions/original./autoimmersion-module_benefit3.jpg"} "全面的软件集成，操作简单直观")
全面的软件集成，操作简单直观
自动加水模块无缝集成至蔡司ZEN成像软件以及蔡司Axio Observer 7的控制触摸屏，这意味着可以简单直观地进行操作，并减少用户干预，甚至实现了远程用户操作。在手动或远程成像实验中，或在自动成像实验中按预先设定的时间间隔，按需应用介质，所有这些都无需接触显微镜。
请参阅运行中的蔡司自动加水模块
解放双手的自动加水功能
![解放双手的自动加水功能，自始至终实现可靠的数据采集]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_autoimmersion-module.jpg/_jcr_content/renditions/original.image_file.100.56.file/lazy-bgrd_autoimmersion-module.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_autoimmersion-module.jpg/_jcr_content/renditions/original.image_file.360.203.file/lazy-bgrd_autoimmersion-module.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_autoimmersion-module.jpg/_jcr_content/renditions/original.image_file.768.432.file/lazy-bgrd_autoimmersion-module.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_autoimmersion-module.jpg/_jcr_content/renditions/original.image_file.1024.576.file/lazy-bgrd_autoimmersion-module.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_autoimmersion-module.jpg/_jcr_content/renditions/original.image_file.1280.720.file/lazy-bgrd_autoimmersion-module.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_autoimmersion-module.jpg/_jcr_content/renditions/original.image_file.1440.810.file/lazy-bgrd_autoimmersion-module.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_autoimmersion-module.jpg/_jcr_content/renditions/original./lazy-bgrd_autoimmersion-module.jpg"} "蔡司Axio Observer 7的自动加水模块")
![为自动加水准备水镜]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_preparing-a-water-objective.jpg/_jcr_content/renditions/original.image_file.100.56.file/lazy-bgrd_preparing-a-water-objective.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_preparing-a-water-objective.jpg/_jcr_content/renditions/original.image_file.360.203.file/lazy-bgrd_preparing-a-water-objective.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_preparing-a-water-objective.jpg/_jcr_content/renditions/original.image_file.768.432.file/lazy-bgrd_preparing-a-water-objective.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_preparing-a-water-objective.jpg/_jcr_content/renditions/original.image_file.1024.576.file/lazy-bgrd_preparing-a-water-objective.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_preparing-a-water-objective.jpg/_jcr_content/renditions/original.image_file.1280.720.file/lazy-bgrd_preparing-a-water-objective.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_preparing-a-water-objective.jpg/_jcr_content/renditions/original.image_file.1440.810.file/lazy-bgrd_preparing-a-water-objective.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_preparing-a-water-objective.jpg/_jcr_content/renditions/original./lazy-bgrd_preparing-a-water-objective.jpg"} "为自动加水准备水镜")
![创建自动加水实验]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_creating-an-experiment.jpg/_jcr_content/renditions/original.image_file.100.56.file/lazy-bgrd_creating-an-experiment.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_creating-an-experiment.jpg/_jcr_content/renditions/original.image_file.360.203.file/lazy-bgrd_creating-an-experiment.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_creating-an-experiment.jpg/_jcr_content/renditions/original.image_file.768.432.file/lazy-bgrd_creating-an-experiment.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_creating-an-experiment.jpg/_jcr_content/renditions/original.image_file.1024.576.file/lazy-bgrd_creating-an-experiment.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_creating-an-experiment.jpg/_jcr_content/renditions/original.image_file.1280.720.file/lazy-bgrd_creating-an-experiment.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_creating-an-experiment.jpg/_jcr_content/renditions/original.image_file.1440.810.file/lazy-bgrd_creating-an-experiment.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_creating-an-experiment.jpg/_jcr_content/renditions/original./lazy-bgrd_creating-an-experiment.jpg"} "创建自动加水实验")
![在物镜间切换移液器]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_switching-feed-pipette.jpg/_jcr_content/renditions/original.image_file.100.56.file/lazy-bgrd_switching-feed-pipette.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_switching-feed-pipette.jpg/_jcr_content/renditions/original.image_file.360.203.file/lazy-bgrd_switching-feed-pipette.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_switching-feed-pipette.jpg/_jcr_content/renditions/original.image_file.768.432.file/lazy-bgrd_switching-feed-pipette.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_switching-feed-pipette.jpg/_jcr_content/renditions/original.image_file.1024.576.file/lazy-bgrd_switching-feed-pipette.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_switching-feed-pipette.jpg/_jcr_content/renditions/original.image_file.1280.720.file/lazy-bgrd_switching-feed-pipette.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_switching-feed-pipette.jpg/_jcr_content/renditions/original.image_file.1440.810.file/lazy-bgrd_switching-feed-pipette.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_switching-feed-pipette.jpg/_jcr_content/renditions/original./lazy-bgrd_switching-feed-pipette.jpg"} "在物镜间切换移液器")
![更换物镜间的自动加水设备]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_cganging-autoimmersion-equipment-between-objectives.jpg/_jcr_content/renditions/original.image_file.100.56.file/lazy-bgrd_cganging-autoimmersion-equipment-between-objectives.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_cganging-autoimmersion-equipment-between-objectives.jpg/_jcr_content/renditions/original.image_file.360.203.file/lazy-bgrd_cganging-autoimmersion-equipment-between-objectives.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_cganging-autoimmersion-equipment-between-objectives.jpg/_jcr_content/renditions/original.image_file.768.432.file/lazy-bgrd_cganging-autoimmersion-equipment-between-objectives.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_cganging-autoimmersion-equipment-between-objectives.jpg/_jcr_content/renditions/original.image_file.1024.576.file/lazy-bgrd_cganging-autoimmersion-equipment-between-objectives.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_cganging-autoimmersion-equipment-between-objectives.jpg/_jcr_content/renditions/original.image_file.1280.720.file/lazy-bgrd_cganging-autoimmersion-equipment-between-objectives.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_cganging-autoimmersion-equipment-between-objectives.jpg/_jcr_content/renditions/original.image_file.1440.810.file/lazy-bgrd_cganging-autoimmersion-equipment-between-objectives.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/lazy-bgrd_cganging-autoimmersion-equipment-between-objectives.jpg/_jcr_content/renditions/original./lazy-bgrd_cganging-autoimmersion-equipment-between-objectives.jpg"} "更换物镜间的自动加水设备")
为复杂实验进行自动加水
提高效率和通量
![通过设计在非工作时间内（如通宵或周末）采集数据的实验，您可将通量提高2.5倍。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/automated-immersion-increased-throughput.png/_jcr_content/renditions/original.image_file.100.56.file/automated-immersion-increased-throughput.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/automated-immersion-increased-throughput.png/_jcr_content/renditions/original.image_file.360.203.file/automated-immersion-increased-throughput.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/automated-immersion-increased-throughput.png/_jcr_content/renditions/original.image_file.768.432.file/automated-immersion-increased-throughput.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/automated-immersion-increased-throughput.png/_jcr_content/renditions/original.image_file.1024.576.file/automated-immersion-increased-throughput.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/automated-immersion-increased-throughput.png/_jcr_content/renditions/original.image_file.1280.720.file/automated-immersion-increased-throughput.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/automated-immersion-increased-throughput.png/_jcr_content/renditions/original.image_file.1440.810.file/automated-immersion-increased-throughput.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/automated-immersion-increased-throughput.png/_jcr_content/renditions/original./automated-immersion-increased-throughput.png"} "通过设计在非工作时间内（如通宵或周末）采集数据的实验，您可将通量提高2.5倍。")
通过设计在非工作时间内（如通宵或周末）采集数据的实验，您可将通量提高2.5倍。
通过设计在非工作时间内（如通宵或周末）采集数据的实验，您可将通量提高2.5倍。
充分利用您的时间和非工作时间
和以往必须待在显微镜附近，确保始终有足够的介质相比，您现可设计复杂的实验，进行无人值守的数据采集，包括扩展的活细胞成像实验和/或多位置数据采集。如此一来，您在显微镜自动收集数据时，便可有时间进行其他工作。蔡司自动加水模块将为进行可靠的数据采集提供保障，直至您的实验结束。
借助自动加水模块，我可以提高显微镜的使用率。白天，用户可以进行短时实验或优化成像参数，而晚间还能自动进行长时间实验。
![Aurélie Jost博士]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/aurelie-jost.jpg/_jcr_content/renditions/original.image_file.100.100.file/aurelie-jost.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/aurelie-jost.jpg/_jcr_content/renditions/original.image_file.360.360.file/aurelie-jost.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/aurelie-jost.jpg/_jcr_content/renditions/original./aurelie-jost.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/aurelie-jost.jpg/_jcr_content/renditions/original./aurelie-jost.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/aurelie-jost.jpg/_jcr_content/renditions/original./aurelie-jost.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/aurelie-jost.jpg/_jcr_content/renditions/original./aurelie-jost.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/aurelie-jost.jpg/_jcr_content/renditions/original./aurelie-jost.jpg"} "Aurélie Jost博士")
蔡司自动加水模块应用案例
应用实例
![HEK KO PEX5细胞通过光笼过氧化物酶体靶向信号1型表达eGFP，由过氧化酶体输入受体PEX5重组。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/hek-ko-pex5-cells-autoimmersion-experiment.jpg/_jcr_content/renditions/original.image_file.100.33.file/hek-ko-pex5-cells-autoimmersion-experiment.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/hek-ko-pex5-cells-autoimmersion-experiment.jpg/_jcr_content/renditions/original.image_file.360.120.file/hek-ko-pex5-cells-autoimmersion-experiment.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/hek-ko-pex5-cells-autoimmersion-experiment.jpg/_jcr_content/renditions/original.image_file.768.256.file/hek-ko-pex5-cells-autoimmersion-experiment.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/hek-ko-pex5-cells-autoimmersion-experiment.jpg/_jcr_content/renditions/original.image_file.1024.341.file/hek-ko-pex5-cells-autoimmersion-experiment.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/hek-ko-pex5-cells-autoimmersion-experiment.jpg/_jcr_content/renditions/original.image_file.1280.427.file/hek-ko-pex5-cells-autoimmersion-experiment.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/hek-ko-pex5-cells-autoimmersion-experiment.jpg/_jcr_content/renditions/original.image_file.1440.480.file/hek-ko-pex5-cells-autoimmersion-experiment.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/hek-ko-pex5-cells-autoimmersion-experiment.jpg/_jcr_content/renditions/original./hek-ko-pex5-cells-autoimmersion-experiment.jpg"} "HEK KO PEX5细胞通过光笼过氧化物酶体靶向信号1型表达eGFP，由过氧化酶体输入受体PEX5重组。")
样品由德国耶拿大学应用光学和生物物理研究院的K. Reglinski提供
使用自动加水模块进行长时间活细胞实验
HEK KO PEX5细胞通过光笼过氧化物酶体靶向信号1型表达eGFP，由过氧化酶体输入受体PEX5重组。光笼的光诱导构象变化导致了过氧化物酶体靶向信号的暴露。如果WT PEX5得以表达，即能监控eGFP信号在点状过氧化物酶体中的堆积（上排图像）。如为突变的PEX5（下排图像），即使在18个小时后，也无法检测到过氧化物酶体。
![细胞内纳米颗粒的吸收]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/macrophages-nanoparticles-uptake-autoimmersion-experiment.jpg/_jcr_content/renditions/original.image_file.100.33.file/macrophages-nanoparticles-uptake-autoimmersion-experiment.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/macrophages-nanoparticles-uptake-autoimmersion-experiment.jpg/_jcr_content/renditions/original.image_file.360.120.file/macrophages-nanoparticles-uptake-autoimmersion-experiment.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/macrophages-nanoparticles-uptake-autoimmersion-experiment.jpg/_jcr_content/renditions/original.image_file.768.256.file/macrophages-nanoparticles-uptake-autoimmersion-experiment.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/macrophages-nanoparticles-uptake-autoimmersion-experiment.jpg/_jcr_content/renditions/original.image_file.1024.341.file/macrophages-nanoparticles-uptake-autoimmersion-experiment.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/macrophages-nanoparticles-uptake-autoimmersion-experiment.jpg/_jcr_content/renditions/original.image_file.1280.427.file/macrophages-nanoparticles-uptake-autoimmersion-experiment.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/macrophages-nanoparticles-uptake-autoimmersion-experiment.jpg/_jcr_content/renditions/original.image_file.1440.480.file/macrophages-nanoparticles-uptake-autoimmersion-experiment.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/macrophages-nanoparticles-uptake-autoimmersion-experiment.jpg/_jcr_content/renditions/original./macrophages-nanoparticles-uptake-autoimmersion-experiment.jpg"} "细胞内纳米颗粒的吸收")
样品提供者：德国耶拿大学应用光学和生物物理研究院的F. Páez Larios和C. Eggeling
使用自动加水模块进行多位置、长时间序列实验
研究活体样品时，您可能不知道您感兴趣的实验现象发生在何处。为了捕捉巨噬细胞对纳米颗粒的吸收，需要在37 °C下使用重新加水的方法对多孔板的多个位置，以及多个Z平面进行长达数小时的图像采集。上方显示的区域是使用自动成像技术采集到的更大的数据集的一个子集，显示了细胞内对纳米颗粒的吸收情况（上排图像）。对细胞表面也进行了成像，以验证纳米颗粒位于细胞内，而不只是位于细胞表面（下排图像）。
![红色荧光小脂质体和不同浓度的Sar1p蛋白（部分使用Alexa Fluor 488标记）在96多孔板中混合，在超过15个小时的时间内自动测量。Krüger et al., Biophys.J. 2017.]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/binding-curve-red-fluorescent-liposomes.png/_jcr_content/renditions/original.image_file.100.63.0,40,1920,1240.file/binding-curve-red-fluorescent-liposomes.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/binding-curve-red-fluorescent-liposomes.png/_jcr_content/renditions/original.image_file.360.225.0,40,1920,1240.file/binding-curve-red-fluorescent-liposomes.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/binding-curve-red-fluorescent-liposomes.png/_jcr_content/renditions/original.image_file.768.480.0,40,1920,1240.file/binding-curve-red-fluorescent-liposomes.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/binding-curve-red-fluorescent-liposomes.png/_jcr_content/renditions/original.image_file.1024.640.0,40,1920,1240.file/binding-curve-red-fluorescent-liposomes.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/binding-curve-red-fluorescent-liposomes.png/_jcr_content/renditions/original.image_file.1280.800.0,40,1920,1240.file/binding-curve-red-fluorescent-liposomes.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/binding-curve-red-fluorescent-liposomes.png/_jcr_content/renditions/original.image_file.1440.900.0,40,1920,1240.file/binding-curve-red-fluorescent-liposomes.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/binding-curve-red-fluorescent-liposomes.png/_jcr_content/renditions/original.image_file.1920.1200.0,40,1920,1240.file/binding-curve-red-fluorescent-liposomes.png"} "红色荧光小脂质体和不同浓度的Sar1p蛋白（部分使用Alexa Fluor 488标记）在96多孔板中混合，在超过15个小时的时间内自动测量。Krüger et al., Biophys.J. 2017.")
![红色荧光小脂质体和不同浓度的Sar1p蛋白（部分使用Alexa Fluor 488标记）在96多孔板中混合，在超过15个小时的时间内自动测量。Krüger et al., Biophys.J. 2017. 样品由德国哈勒大学的C. Haupt和K. Bacia提供]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/binding-curve-red-fluorescent-liposomes.png/_jcr_content/renditions/original.image_file.100.67.file/binding-curve-red-fluorescent-liposomes.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/binding-curve-red-fluorescent-liposomes.png/_jcr_content/renditions/original.image_file.360.240.file/binding-curve-red-fluorescent-liposomes.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/binding-curve-red-fluorescent-liposomes.png/_jcr_content/renditions/original.image_file.768.512.file/binding-curve-red-fluorescent-liposomes.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/binding-curve-red-fluorescent-liposomes.png/_jcr_content/renditions/original.image_file.1024.683.file/binding-curve-red-fluorescent-liposomes.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/binding-curve-red-fluorescent-liposomes.png/_jcr_content/renditions/original.image_file.1280.853.file/binding-curve-red-fluorescent-liposomes.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/binding-curve-red-fluorescent-liposomes.png/_jcr_content/renditions/original.image_file.1440.960.file/binding-curve-red-fluorescent-liposomes.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/autoimmersion-module/binding-curve-red-fluorescent-liposomes.png/_jcr_content/renditions/original./binding-curve-red-fluorescent-liposomes.png"} "红色荧光小脂质体和不同浓度的Sar1p蛋白（部分使用Alexa Fluor 488标记）在96多孔板中混合，在超过15个小时的时间内自动测量。Krüger et al., Biophys.J. 2017. 样品由德国哈勒大学的C. Haupt和K. Bacia提供")
红色荧光小脂质体和不同浓度的Sar1p蛋白（部分使用Alexa Fluor 488标记）在96多孔板中混合，在超过15个小时的时间内自动测量。Krüger et al., Biophys.J. 2017.
红色荧光小脂质体和不同浓度的Sar1p蛋白（部分使用Alexa Fluor 488标记）在96多孔板中混合，在超过15个小时的时间内自动测量。Krüger et al., Biophys.J. 2017.样品由德国哈勒大学的C. Haupt和K. Bacia提供
水相样品中的可靠数据采集
蔡司自动加水模块快速又准确，可确保您的数据采集无误，即使将多孔样品移动至多个位置时也是如此。如图所示，研究人员通过不同浓度的荧光标记蛋白来制备孔，并能够使用荧光交叉相关光谱（FCCS）准确测量与红色荧光脂质体的结合曲线。
![ZEISS Axio Observer的预览图像]({"max":"https://asset-downloads.zeiss.com/catalogs/preview/mic/3a8e2b42-6dc6-45f5-9dbe-ae81a7f245b8/EN_product-info_Axio-Observer_Bio.pdf.jpg"} "下载ZEISS Axio Observer")
![蔡司Axio Observer的预览图像]({"max":"https://asset-downloads.zeiss.com/catalogs/preview/mic/05f3c6df-26a7-4c94-9c06-b13840fa7596/CN_product-info_Axio-Observer.pdf.jpg"} "下载蔡司Axio Observer")
![Autoimmersion Module for ZEISS Axio Observer 7的预览图像]({"max":"https://asset-downloads.zeiss.com/catalogs/preview/mic/97fa2074-a1eb-47a1-9083-820b82350213/EN_product-flyer_Autoimmersion_Module.pdf.jpg"} "下载Autoimmersion Module for ZEISS Axio Observer 7")
![用于生命科学研究的蔡司Axio Observer]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/axio-observer-ls_product.jpg/_jcr_content/renditions/original.image_file.100.100.420,0,1500,1080.file/axio-observer-ls_product.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/axio-observer-ls_product.jpg/_jcr_content/renditions/original.image_file.360.360.420,0,1500,1080.file/axio-observer-ls_product.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/axio-observer-ls_product.jpg/_jcr_content/renditions/original.image_file.768.768.420,0,1500,1080.file/axio-observer-ls_product.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/axio-observer-ls_product.jpg/_jcr_content/renditions/original.image_file.1024.1024.420,0,1500,1080.file/axio-observer-ls_product.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/axio-observer-ls_product.jpg/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/axio-observer-ls_product.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/axio-observer-ls_product.jpg/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/axio-observer-ls_product.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/widefield/axio-observer-l/axio-observer-ls_product.jpg/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/axio-observer-ls_product.jpg"} "用于生命科学研究的蔡司Axio Observer")
![在高帧率下图像不仅可拥有4 - 8倍更高的信噪比，还具有超分辨率。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-900/lsm-900-confocal-microscope.jpg/_jcr_content/renditions/original.image_file.100.100.file/lsm-900-confocal-microscope.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-900/lsm-900-confocal-microscope.jpg/_jcr_content/renditions/original.image_file.360.360.file/lsm-900-confocal-microscope.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-900/lsm-900-confocal-microscope.jpg/_jcr_content/renditions/original.image_file.768.768.file/lsm-900-confocal-microscope.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-900/lsm-900-confocal-microscope.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/lsm-900-confocal-microscope.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-900/lsm-900-confocal-microscope.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/lsm-900-confocal-microscope.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-900/lsm-900-confocal-microscope.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/lsm-900-confocal-microscope.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-900/lsm-900-confocal-microscope.jpg/_jcr_content/renditions/original./lsm-900-confocal-microscope.jpg"} "采用Airyscan 2技术的蔡司LSM 900")
![采用Airyscan 2技术的蔡司LSM 980]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-980-airyscan-2/lsm-980-airyscan-stage.jpg/_jcr_content/renditions/original.image_file.100.100.file/lsm-980-airyscan-stage.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-980-airyscan-2/lsm-980-airyscan-stage.jpg/_jcr_content/renditions/original.image_file.360.360.file/lsm-980-airyscan-stage.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-980-airyscan-2/lsm-980-airyscan-stage.jpg/_jcr_content/renditions/original.image_file.768.768.file/lsm-980-airyscan-stage.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-980-airyscan-2/lsm-980-airyscan-stage.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/lsm-980-airyscan-stage.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-980-airyscan-2/lsm-980-airyscan-stage.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/lsm-980-airyscan-stage.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-980-airyscan-2/lsm-980-airyscan-stage.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/lsm-980-airyscan-stage.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-980-airyscan-2/lsm-980-airyscan-stage.jpg/_jcr_content/renditions/original./lsm-980-airyscan-stage.jpg"} "采用Airyscan 2技术的蔡司LSM 980")