![蔡司Spectral RICS]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics.jpg/_jcr_content/renditions/original.image_file.100.100.file/spectral-rics.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics.jpg/_jcr_content/renditions/original.image_file.360.360.file/spectral-rics.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics.jpg/_jcr_content/renditions/original.image_file.768.768.file/spectral-rics.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/spectral-rics.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics.jpg/_jcr_content/renditions/original./spectral-rics.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics.jpg/_jcr_content/renditions/original./spectral-rics.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics.jpg/_jcr_content/renditions/original./spectral-rics.jpg"} "蔡司Spectral RICS")
蔡司Spectral RICS
描绘细胞环境中的分子相互作用分布情况
蔡司Spectral RICS将LSM成像与蛋白质在细胞环境中的行为信息结合起来，有助于识别呈现出不同分子特征的区域。通过特殊的光谱拆分功能，Spectral RICS为研究蛋白质之间的结合行为提供了良好的基础。
![原始时间序列（左）和栅格热图（右）。RICS分析显示，EGFP在核仁内的扩散速度比在核质中更慢。样品由德国耶拿莱布尼茨老龄化研究所影像中心的P. Hemmerich和T. Ulbricht友情提供。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-egfp-diffusion.jpg/_jcr_content/renditions/original.image_file.100.56.file/rics-egfp-diffusion.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-egfp-diffusion.jpg/_jcr_content/renditions/original.image_file.360.203.file/rics-egfp-diffusion.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-egfp-diffusion.jpg/_jcr_content/renditions/original.image_file.768.432.file/rics-egfp-diffusion.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-egfp-diffusion.jpg/_jcr_content/renditions/original.image_file.1024.576.file/rics-egfp-diffusion.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-egfp-diffusion.jpg/_jcr_content/renditions/original.image_file.1280.720.file/rics-egfp-diffusion.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-egfp-diffusion.jpg/_jcr_content/renditions/original.image_file.1440.810.file/rics-egfp-diffusion.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-egfp-diffusion.jpg/_jcr_content/renditions/original./rics-egfp-diffusion.jpg"} "原始时间序列（左）和栅格热图（右）")
原始时间序列（左）和栅格热图（右）。RICS分析显示，EGFP在核仁内的扩散速度比在核质中更慢。样品由德国耶拿莱布尼茨老龄化研究所影像中心的P. Hemmerich和T. Ulbricht友情提供。
原始时间序列（左）和栅格热图（右）。RICS分析显示，EGFP在核仁内的扩散速度比在核质中更慢。样品由德国耶拿莱布尼茨老龄化研究所影像中心的P. Hemmerich和T. Ulbricht友情提供。
光栅图像相关光谱
在细胞中研究分子动力学
光栅图像相关光谱（RICS）是一种先进的生物成像技术，用于研究细胞内的分子和颗粒动力学，包括通过样品的光栅扫描图像分析荧光标记颗粒的运动。通过关联图像中不同像素之间的强度波动，RICS可以提供有关这些颗粒的扩散速率、浓度和相互作用的信息。
![通过光谱拆分后的RICS分析，可以证明两种蛋白质之间假定的相互作用并不存在。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics-protein-interaction.jpg/_jcr_content/renditions/original.image_file.100.75.file/spectral-rics-protein-interaction.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics-protein-interaction.jpg/_jcr_content/renditions/original.image_file.360.270.file/spectral-rics-protein-interaction.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics-protein-interaction.jpg/_jcr_content/renditions/original.image_file.768.576.file/spectral-rics-protein-interaction.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics-protein-interaction.jpg/_jcr_content/renditions/original.image_file.1024.768.file/spectral-rics-protein-interaction.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics-protein-interaction.jpg/_jcr_content/renditions/original.image_file.1280.960.file/spectral-rics-protein-interaction.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics-protein-interaction.jpg/_jcr_content/renditions/original.image_file.1440.1080.file/spectral-rics-protein-interaction.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics-protein-interaction.jpg/_jcr_content/renditions/original./spectral-rics-protein-interaction.jpg"} "Spectral RICS的优势")
通过光谱拆分后的RICS分析，可以证明两种蛋白质之间假定的相互作用并不存在。样品由哈瑟尔特大学生物医学研究所高级光学显微镜中心动态生物成像实验室的Jelle Hendrix教授友情提供。
通过光谱拆分后的RICS分析，可以证明两种蛋白质之间假定的相互作用并不存在。样品由哈瑟尔特大学生物医学研究所高级光学显微镜中心动态生物成像实验室的Jelle Hendrix教授友情提供。
Spectral RICS的优势
揭示蛋白质在活细胞中的真实行为
蔡司Spectral RICS是与比利时哈瑟尔特大学的Jelle Hendrix教授合作研发的成果。它将光谱拆分功能添加到RICS方法中，可避免将重叠光谱误判为蛋白质相互作用。通过光谱拆分，您可以确保获得无偏差的信息，揭示蛋白质在活细胞中的真实行为。
蔡司Spectral RICS以ZEN扩展功能中的一个工作流来实现，从实验设置到数据分析的整个过程中，都可为您提供引导。其包括光谱拆分、自动和互相关分析、强度和栅格热图以及基于时间变化的分析，并结合了ROI选择和强度阈值等工具。
蔡司Spectral RICS应用实例
![SUMO化对蛋白质扩散的影响]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-sumoylation-protein-diffusion.jpg/_jcr_content/renditions/original.image_file.100.67.60,0,1860,1200.file/rics-sumoylation-protein-diffusion.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-sumoylation-protein-diffusion.jpg/_jcr_content/renditions/original.image_file.360.240.60,0,1860,1200.file/rics-sumoylation-protein-diffusion.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-sumoylation-protein-diffusion.jpg/_jcr_content/renditions/original.image_file.768.512.60,0,1860,1200.file/rics-sumoylation-protein-diffusion.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-sumoylation-protein-diffusion.jpg/_jcr_content/renditions/original.image_file.1024.683.60,0,1860,1200.file/rics-sumoylation-protein-diffusion.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-sumoylation-protein-diffusion.jpg/_jcr_content/renditions/original.image_file.1280.853.60,0,1860,1200.file/rics-sumoylation-protein-diffusion.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-sumoylation-protein-diffusion.jpg/_jcr_content/renditions/original.image_file.1440.960.60,0,1860,1200.file/rics-sumoylation-protein-diffusion.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-sumoylation-protein-diffusion.jpg/_jcr_content/renditions/original.image_file.1800.1200.60,0,1860,1200.file/rics-sumoylation-protein-diffusion.jpg"} "SUMO化对蛋白质扩散的影响")
![SUMO化对蛋白质扩散的影响]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-sumoylation-protein-diffusion.jpg/_jcr_content/renditions/original.image_file.100.63.file/rics-sumoylation-protein-diffusion.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-sumoylation-protein-diffusion.jpg/_jcr_content/renditions/original.image_file.360.225.file/rics-sumoylation-protein-diffusion.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-sumoylation-protein-diffusion.jpg/_jcr_content/renditions/original.image_file.768.480.file/rics-sumoylation-protein-diffusion.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-sumoylation-protein-diffusion.jpg/_jcr_content/renditions/original.image_file.1024.640.file/rics-sumoylation-protein-diffusion.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-sumoylation-protein-diffusion.jpg/_jcr_content/renditions/original.image_file.1280.800.file/rics-sumoylation-protein-diffusion.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-sumoylation-protein-diffusion.jpg/_jcr_content/renditions/original.image_file.1440.900.file/rics-sumoylation-protein-diffusion.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-sumoylation-protein-diffusion.jpg/_jcr_content/renditions/original./rics-sumoylation-protein-diffusion.jpg"} "SUMO化对蛋白质扩散的影响")
样品由德国耶拿莱布尼茨老龄化研究所影像中心的P. Hemmerich和T. Ulbricht友情提供。
样品由德国耶拿莱布尼茨老龄化研究所影像中心的P. Hemmerich和T. Ulbricht友情提供。
SUMO化对蛋白质扩散的影响
RICS可用于测量蛋白质相互作用导致的分子扩散变化。通过标准自相关RICS分析，可以看到扩散系数随着SUMO链的变小而下降。此类研究还可以测量感兴趣的标记蛋白质在药物治疗、突变或其他影响下的扩散变化。
![LEDGF与组蛋白的相互作用]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics-ledgf-histone-interaction.jpg/_jcr_content/renditions/original.image_file.100.56.file/spectral-rics-ledgf-histone-interaction.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics-ledgf-histone-interaction.jpg/_jcr_content/renditions/original.image_file.360.203.file/spectral-rics-ledgf-histone-interaction.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics-ledgf-histone-interaction.jpg/_jcr_content/renditions/original.image_file.768.432.file/spectral-rics-ledgf-histone-interaction.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics-ledgf-histone-interaction.jpg/_jcr_content/renditions/original.image_file.1024.576.file/spectral-rics-ledgf-histone-interaction.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics-ledgf-histone-interaction.jpg/_jcr_content/renditions/original.image_file.1280.720.file/spectral-rics-ledgf-histone-interaction.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics-ledgf-histone-interaction.jpg/_jcr_content/renditions/original.image_file.1440.810.file/spectral-rics-ledgf-histone-interaction.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/spectral-rics-ledgf-histone-interaction.jpg/_jcr_content/renditions/original./spectral-rics-ledgf-histone-interaction.jpg"} "LEDGF与组蛋白的相互作用")
样品由比利时哈瑟尔特大学生物医学研究所高级光学显微镜中心动态生物成像实验室的Jelle Hendrix教授友情提供。
样品由比利时哈瑟尔特大学生物医学研究所高级光学显微镜中心动态生物成像实验室的Jelle Hendrix教授友情提供。
LEDGF与组蛋白的相互作用
LEDGF是一种染色质结合转录因子，而H2B是一种帮助排布真核DNA的组蛋白。在进行光谱拆分之前，对这两种蛋白质进行的RICS互相关分析表明它们之间存在很强的相互作用。然而，光谱拆分显示，在大多数细胞核中，这两种蛋白质的相互作用非常小。
![探索EGFR在呼肠孤病毒结合位点的行为]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-egfp-reovirus.jpg/_jcr_content/renditions/original.image_file.100.56.file/rics-egfp-reovirus.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-egfp-reovirus.jpg/_jcr_content/renditions/original.image_file.360.203.file/rics-egfp-reovirus.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-egfp-reovirus.jpg/_jcr_content/renditions/original.image_file.768.432.file/rics-egfp-reovirus.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-egfp-reovirus.jpg/_jcr_content/renditions/original.image_file.1024.576.file/rics-egfp-reovirus.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-egfp-reovirus.jpg/_jcr_content/renditions/original.image_file.1280.720.file/rics-egfp-reovirus.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-egfp-reovirus.jpg/_jcr_content/renditions/original.image_file.1440.810.file/rics-egfp-reovirus.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/spectral-rics/rics-egfp-reovirus.jpg/_jcr_content/renditions/original./rics-egfp-reovirus.jpg"} "探索EGFR在呼肠孤病毒结合位点的行为")
样品由比利时鲁汶大学纳米生物物理实验室的J.D. Simpson和D. Alsteens友情提供。
样品由比利时鲁汶大学纳米生物物理实验室的J.D. Simpson和D. Alsteens友情提供。
探索EGFR在呼肠孤病毒结合位点的行为
表皮生长因子受体（EGFR）在细胞膜上表达，并且是呼肠孤病毒入侵的受体之一。呼肠孤病毒着陆和结合位点以品红色显示。以栅格热图形式展示的RICS分析表明，与其他膜位置相比，EGFR在病毒结合位点附近的扩散速度更快。
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ZEISS Spectral RICS
Your Solution for Mapping Molecular Interactions in the Cellular Environment
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![ZEISS Spectral RICS的预览图像]({"max":"https://asset-downloads.zeiss.com/catalogs/preview/mic/03fa1f6a-e169-486d-92d1-cb3dd266456a/EN_product-flyer_ZEISS-Spectral-RICS.pdf.jpg"} "下载ZEISS Spectral RICS")
![蔡司Dynamics Profiler]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/dynamics-profiler/dynamics-profiler.jpg/_jcr_content/renditions/original.image_file.100.100.file/dynamics-profiler.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/dynamics-profiler/dynamics-profiler.jpg/_jcr_content/renditions/original.image_file.360.360.file/dynamics-profiler.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/dynamics-profiler/dynamics-profiler.jpg/_jcr_content/renditions/original.image_file.768.768.file/dynamics-profiler.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/dynamics-profiler/dynamics-profiler.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/dynamics-profiler.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/dynamics-profiler/dynamics-profiler.jpg/_jcr_content/renditions/original./dynamics-profiler.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/dynamics-profiler/dynamics-profiler.jpg/_jcr_content/renditions/original./dynamics-profiler.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/zen/dynamics-profiler/dynamics-profiler.jpg/_jcr_content/renditions/original./dynamics-profiler.jpg"} "蔡司Dynamics Profiler")
![采用Airyscan 2技术的蔡司LSM 980]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-980-airyscan-2/lsm-980-airyscan-stage.jpg/_jcr_content/renditions/original.image_file.100.100.file/lsm-980-airyscan-stage.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-980-airyscan-2/lsm-980-airyscan-stage.jpg/_jcr_content/renditions/original.image_file.360.360.file/lsm-980-airyscan-stage.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-980-airyscan-2/lsm-980-airyscan-stage.jpg/_jcr_content/renditions/original.image_file.768.768.file/lsm-980-airyscan-stage.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-980-airyscan-2/lsm-980-airyscan-stage.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/lsm-980-airyscan-stage.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-980-airyscan-2/lsm-980-airyscan-stage.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/lsm-980-airyscan-stage.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-980-airyscan-2/lsm-980-airyscan-stage.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/lsm-980-airyscan-stage.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/light/confocal/lsm-980-airyscan-2/lsm-980-airyscan-stage.jpg/_jcr_content/renditions/original./lsm-980-airyscan-stage.jpg"} "采用Airyscan 2技术的蔡司LSM 980")